|
|
| Line 59: |
Line 59: |
| | </tr> | | </tr> |
| | <tr><td colspan=6 align=left valign=center> | | <tr><td colspan=6 align=left valign=center> |
| − | <font size=7 color=#51a7f9><b style="color: #51a7f9">Collaborations</b></font> | + | <font size=7 color=#51a7f9><b style="color: #51a7f9">Interview with Dr. Keith Pardee</b></font> |
| | </td> | | </td> |
| − | </tr>
| |
| − | <tr>
| |
| − | <td colspan = 6 align="left">
| |
| − | <p class="introduction">
| |
| − | Collaborations play a very important role in terms of the development of project. Collaborations with other teams helps us to learn about better ways to handle a problem, to learn new ways of working, to perceive different ideologies and to develop the project in general. It provides us a better chance to get to know other teams and to learn to cooperate. In scientific fields, cooperation and collaborations play a major role for growth and discovery. We are highly encouraged to work with other teams since it increases our horizon of knowledge and we are happy that iGEM promotes the idea of sharing knowledge and scientific materials. The following are the teams whom we can proudly call the collaborators this year.
| |
| − | </p>
| |
| − | </td>
| |
| | </tr> | | </tr> |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| | | | |
| | <tr class="lastRow"><td colspan=4 align=center valign=center> | | <tr class="lastRow"><td colspan=4 align=center valign=center> |
| − | <h3><a class="myLink" href="/Team:TUDelft">iGEM TU Delft</a></h3> | + | <h3>We read your paper from last year titled “Rapid, Low-Cost Detection of Zika Virus Using |
| | + | Programmable Biomolecular Components” and we wanted to know, what led you to |
| | + | use β-galactosidase as a colored readout?</h3> |
| | <p> | | <p> |
| − | Their iGEM project is called CASE13A. Both our projects are similar in terms of the use of Cas13a and paper microfluidics. Our collaboration started with our meeting in Delft. We were excited to see that TU Delft were also working with Cas13a as their major protein. We both are trying to work on different ways of tackling the problem of the antibiotic resistance using Cas13a. Therefore we decided to collaborate since it gave us the opportunity to discuss the challenges and also to try out new stuffs together. We started a collaboration for our <a class="myLink" href="/Team:Munich/Software">software</a> since we both were working on optimizing the crRNA for the different targets. In our team, we designed a software that could give us the best design and structure of the crRNA for different targets. For this we created a database of different possible sequences using NUPACK and other platforms. The team Delft had a similar project where they predict the part of the target that can best serve as a crRNA. We provided them with a list of possible targets and best crRNAs structures for their software. Also, the team Delft sent us the Tardigrade proteins(TDPs) to experiment them with the Cas13a and to check the activity and stability of the Cas13a when used together with TDPs. We did some cleavage assay of the Cas13a along with the TDPs, to see if it can create some difference in the reactivity.</p>
| + | Dr. Pardee: At that time, we hadn’t considered using C2C2 (Cas13a). So, one of the reasons was |
| − | </td>
| + | because of the enzyme activity. Single molecule of reporter could serve amplification, rather than |
| − | <td colspan=2 align=center valign=center>
| + | just fluorescence. The other (reason) was just for practical use. No need (to use) UV lights, |
| − | <a href="/Team:TUDelft"><img src="https://pbs.twimg.com/profile_images/869900215146487808/51JLvK2L_400x400.jpg" alt="Diagram for Cas13a's function"></a>
| + | electronics, camera, and could be done in a simple piece of paper. Tried 10 different enzyme |
| | + | options with other based reporters.</p> |
| | </td> | | </td> |
| | </tr> | | </tr> |
