Difference between revisions of "Team:TAS Taipei/HP/Gold Integrated"

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         <img src="https://static.igem.org/mediawiki/2017/0/0d/5home.svg" alt="Home" id="home" onclick="location.href='https://2017.igem.org/Team:TAS_Taipei';" style="cursor: pointer;">
 
         <img src="https://static.igem.org/mediawiki/2017/0/0d/5home.svg" alt="Home" id="home" onclick="location.href='https://2017.igem.org/Team:TAS_Taipei';" style="cursor: pointer;">
 
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    <div class="jumbotron" id="hp-jumbo">
 
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            <h1>HP Gold Integrated</h1>
 
            <h4>It’s not only what happens in the lab, but also what happens in our community.</h4>
 
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        <a href="#cv"><img src="https://static.igem.org/mediawiki/2017/4/4a/T--TAS_Taipei--Chevron_500px_200ppi.png" alt="test" id="chevron"></a>
 
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                 <ul class="nav">
 
                 <ul class="nav">
 
                     <li>
 
                     <li>
                         <a href="#roam" class="pageNavSm">Dr. Gwo-Dong Roam</a>
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                         <a href="#bioethics" class="pageNavSm">Bioethics Panel</a>
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                    </li>
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                    <li>
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                        <a href="#Apex" class="pageNavSm">Apex Nanotek</a>
 
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                     <li>
                         <a href="#theps" class="pageNavSm">THEPS Environmental Protection Engineering Company</a>
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                         <a href="#EPA" class="pageNavSm">Thomas J. Brown</a>
                    </li>
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                        <a href="#Fair" class="pageNavSm">Spring Fair</a>
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                    </li>
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                        <a href="#fund" class="pageNavSm">Fundraising and Donation</a>
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                    </li>
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                    <li>
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                        <a href="#pubView" class="pageNavSm">Public Outreach</a>
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                    </li>
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                    <li>
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                        <a href="#survey" class="pageNavSm">Survey Results</a>
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                    </li>
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                    <li>
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                        <a href="#policy" class="pageNavSm">Policy Brief</a>
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                     </li>
 
                     </li>
 
                 </ul>
 
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                 <header>
 
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                     <div class="row" id="HPSummary">
 
                     <div class="row" id="HPSummary">
                         <h1 class="name col-lg-12">HUMAN PRACTICES SILVER</h1>
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                         <h1 class="name col-lg-12">HP GOLD INTEGRATED</h1>
 
                     </div>
 
                     </div>
 
                     <div class="row">
 
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                         <h4 class="para col-lg-12">Our project this year aims to remove nanoparticles in wastewater systems. The increasing use of nanoparticles can potentially become an issue because of nanoparticles small size that can interact with biological systems and their inability to be release out of the system once it enters the body. We interviewed several professors, such as Dr. Roam and Dr. Eric Lee on their viewpoints on nanoparticles. Both of them agreed that nanoparticle is an issue that requires more attention and can be difficult to handle. We went to several different wastewater treatment plant to study on the current purification method as well as the design of the treatment plant. Using the information they have provided, we created our prototype in which our constructs can be used in its full potential. Aside from the lab work, our team also want to raise awareness on the possible health risks associated with the usage as well as the ethical concerns of synthetic biology. We sent out surveys and during Spring Fair and Food Fair and passed out fliers around Taipei to garner public awareness. Our survey results confirmed that the public should be informed more thoroughly on the usage of nanoparticles since many were not aware that there is a potential negative impact associated with nanoparticles.
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                         <h4 class="para col-lg-12">At the beginning of our project, we visited local and foreign wastewater treatment plants and learned that there are currently very few water purification methods that specifically target nanoparticle waste. We designed our constructs, prototype and modeling based directly from information given by these treatment plants. Our biofilm parts collection was designed to be able to control biofilm production in sedimentation tanks. Our proteorhodopsin construct is designed to be used in aeration tanks where other microbes are already breaking down organic substances. We also use the pre-existing biosafety aspect of the wastewater plants.   Additionally, we reached out to several nanoparticle manufacturers, researchers, disposal services and wastewater experts who provided us with information on the advantages and potential consequences of nanoparticle usage, as well as where to target cleaning nanoparticle waste. The feedback we received guided the direction of our project and confirmed the importance of our project to our community.  
 
                         </h4>
 
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                 <section class="main">
 
                 <section class="main">
                    <div class="row" id="roam">
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                                        <div class="row" id="bioethics">
                         <h1 class="section-title col-lg-12">Dr. Gwo-Dong Roam</h1>
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                         <h1 class="section-title col-lg-12">Bioethics Panel</h1>
 
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                             We interviewed Professor Roam of National Central University and former general director of the Environmental Analysis Labs (EAL) of the Taiwan Environmental Protection Agency to learn more about the background and potential threat of nanoparticles. Dr. Roam informed us that the most common nanoparticles used in Taiwan include: TiO2, ZnO, Ag, Au, Fe, Carbon Nanotubes, Fullerenes, Clay, and Graphene. He also told us that the toxicity of a nanoparticle is directly related to its size, but there are currently no regulations or guidelines that specify the toxicity of different types and sizes of nanoparticle. With the increased use of nanoparticles in society, Dr. Roam believes that more attention should be placed on waste management, risk assessment and regulations.
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                             We hosted a Bioethics Panel, where we invited students and teachers to discuss the moral, social and environmental concerns of our project. To encourage participants to consider the problems from multiple perspectives, we created a role-playing game and assigned different roles to participants. We then asked for their opinions on nanoparticle usage and disposal from the perspective of their assigned role. (Whole team activity)
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                            <br><br> For instance, one of our questions was:
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                            <br><br> “Dihua WWTP has no nanoparticle removal plan. Should this be the job of the wastewater plant? Or the nanoparticle manufacturer?”
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                            <br><br> The following roles were assigned:
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                            <ul>
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                                <li>Wastewater plant manager </li>
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                                <li>Nanoparticle manufacturer </li>
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                                <li>Citizen</li>
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                                <li>Fisherman</li>
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                                <li>Fish</li>
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                            </ul>
 
                         </h4>
 
                         </h4>
                    </div>
 
                    <div class="row" id="Dihua">
 
                        <h1 class="section-title col-lg-12">WWTP -- Dihua Wastewater Treatment Plant</h1>
 
 
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                             In order to learn firsthand about the effect of nanoparticles in WWTPs, we visited the Dihua WWTP (迪化污水處理廠). Here, we were given a tour around the plant, and were able to ask questions to the managers and people that work there. They confirmed that the current facilities are unable to remove nanoparticles from wastewater mainly due to their small size. In addition to this information, they kindly provided us with samples of sludge, effluent water, and the polymers they add during wastewater processing. Throughout the year we visited and talked to the Dihua WWTP several times about where and how our project could be implemented in their current system. These conversations and visits played a huge role in shaping our construct design, prototype design, mathematical modeling and overall purpose for our project. (Whole team activity)
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                             Most of the wastewater plant managers thought that nanoparticle manufacturers should be responsible for removing nanoparticles, because they have more information (e.g., solubility, toxicity, etc.) about their own products. <b>However, many other participants were skeptical that manufacturers could be trusted to remove their own contamination and agreed that WWTPs should ultimately be responsible for cleaning water contaminated with nanoparticles.</b>
                        </h4>
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                        <div class="image_container col-lg-4">
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                            <img src="https://static.igem.org/mediawiki/2017/c/ce/T--TAS_Taipei--Dihua_Tank-min.jpg" alt="test" id="group">
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                         </div>
 
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                             <img src="https://static.igem.org/mediawiki/2017/e/e1/T--TAS_Taipei--DihuaDiagram-new.jpg" alt="test" id="group">
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                             This activity gave us great insight on how the public perceives nanoparticle usage and regulation in society. This also gave us a chance to talk to people about both the benefits and the dangers of using nanoparticles.
                            <h4 class="subtitle">We plan to add our bacteria either in the deep aeration tanks or the secondary sedimentation tanks. The disinfection tank will kill the bacteria used in previous tanks.<span class="subCred">Figure: Christine C.</span></h4>
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                         </div>
 
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                        <video controls="" class="col-lg-10 col-lg-offset-1">
 
                            <source src="https://static.igem.org/mediawiki/2017/9/97/T--TAS_Taipei--Dihua_WWTP_%281%29.mp4" type="video/mp4"> Your browser does not support the video tag.
 
                        </video>
 
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                    <div class="row" id="Boswell">
 
                        <h1 class="section-title col-lg-12">Boswell Wastewater Treatment Plant</h1>
 
 
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                     <div class="row">
 
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                         <h4 class="para col-lg-12">
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                         <a href="https://static.igem.org/mediawiki/2017/7/76/T--TAS_Taipei--bioethics_panel_results_pdf.pdf" type="button" class="btn btn-info col-lg-6 col-lg-offset-3">
                             Not all WWTPs are as large as the one in Taipei. One of our advisors (Jude Clapper) went to visit the Boswell WWTP in rural southwestern Pennsylvania. We learned that the same processes that occur in the Taipei Dihua WWTP also occur in the Boswell WWTP, but with different water flow rates and waste quantities. Because of the similarities in how both WWTPs process their wastewater, It inspired us to create our current prototype design that is a rotating polymeric bioreactor coated in biofilm. This prototype will be placed in the secondary sedimentation tank, where the majority of organic solids have been removed and only smaller particles exist. The Boswell WWTP also confirmed that since our project is bacteria-based, it will be killed by UV light and chlorine in the disinfection tank, similar to the Dihua WWTP, before the water turns into effluent and goes to the rivers and oceans.
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                             Click here to see the compiled results from all participants
                         </h4>
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                         </a>
 
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                             <img src="https://static.igem.org/mediawiki/2017/2/2f/T--TAS_Taipei--BoswellDiagram-new.jpg" alt="test" id="group">
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                             <img src="https://static.igem.org/mediawiki/2017/9/97/T--TAS_Taipei--general_questionsPic.JPG" alt="test" id="group">
                            <h4 class="subtitle">We plan to add our bacteria either in the deep aeration tanks or the secondary sedimentation tanks. The disinfection tank will kill the bacteria used in previous tanks.<span class="subCred">Figure: Christine C.</span></h4>
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                     </div>
                     <div class="row" id="theps">
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                     <div class="row" id="apex">
                         <h1 class="section-title col-lg-12">THEPS Environmental Protection Engineering Company (中港環保工程股份有限公司)</h1>
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                         <h1 class="section-title col-lg-12">Apex Nanotek</h1>
 
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                     <div class="row">
 
                     <div class="row">
                         <h4 class="para col-lg-12">
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                         <h4 class="para col-lg-8">
                             We contacted the company that removes our nanoparticle waste because we wanted to know what happens when it leaves our lab. They directed us to National Cheng Kung university who actually treats the waste for them. The university uses chemicals and burning to aggregate nanoparticles. Through literature research, we discovered that burning nanoparticles is the most prevalent way for removal, however it is not 100% effective at removing all types of nanomaterials (Marr et. al. 2013). (Interviewed by Katherine H, Audrey T. and Christine C.)
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                             To learn more about the applications of nanoparticles, we visited a nanotech company that uses silver nanoparticles to make various antimicrobial products. The researcher and manager of Apex Nanotek, Chery Yang, introduced us to their main product, which is antimicrobial nanosilver activated carbon. Pure activated carbon, commonly used to treat sewage and industrial exhaust, is prone to bacterial growth. To overcome this problem, they integrate crystallized nanosilver into the activated carbon for their antimicrobial effects. One of their products is a showerhead, with nanosilver activated carbon filters to kill bacteria when water flows through the showerhead.
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                            <br><br> We tested the product by comparing SEM images between tap water and filtered water from the showerhead. The showerhead decreased the number of bacteria and larger particles from tap water! However, we also observed the release of nanoparticles from the filter, which will flow into wastewater. (Interviewed by Christine C., Kelly C., Yvonne W., Chansie Y., and Justin Y.)
 
                         </h4>
 
                         </h4>
                    </div>
 
                    <div class="row" id="Fair">
 
                        <h1 class="section-title col-lg-12">Spring Fair -- Spreading Public Awareness of Nanoparticles</h1>
 
                    </div>
 
                    <div class="row">
 
                        <h4 class="para col-lg-12">
 
                            At our school’s annual spring fair, we manned a booth where people could create their own glitter slime by mixing polyvinyl alcohol and sodium borate solutions. The slime was meant to simulate the biofilm we use to trap nanoparticles (in this demo, glitter) in wastewater treatment plants. We also showed a few SEM images of bacteria, as well as everyday products that contain nanoparticles such as toothpaste and sunscreen. Everyone who came by our booth was encouraged to take our survey so we could record opinions on bioethics and concerns about nanoparticles. (Whole team activity)
 
                        </h4>
 
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                         <div class="image_container col-lg-4">
 
                         <div class="image_container col-lg-4">
                             <img src="https://static.igem.org/mediawiki/2017/5/53/T--TAS_Taipei--Spring_Fair-min.jpg" alt="test" id="group">
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                             <img src="https://static.igem.org/mediawiki/2017/0/07/T--TAS_Taipei--Group_Pic_Apex-min.jpg" alt="test" id="group">
                             <h4 class="subtitle">iGEM Slime booth at Spring Fair along with the iPad surveys set up next to the tables.</h4>
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                             <h4 class="subtitle">Chery Yang (third person from the left), the main researcher of Apex Nanotek Corporation<span class="subCred"></span></h4>
 
                         </div>
 
                         </div>
                        <div class="image_container col-lg-4">
 
                            <img src="https://static.igem.org/mediawiki/2017/6/61/T--TAS_Taipei--Spring_Fair_Sage-min.jpg" alt="test" id="group">
 
                        </div>
 
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                            <img src="https://static.igem.org/mediawiki/2017/a/a4/T--TAS_Taipei--Spring_Fair_NP-min.jpg" alt="test" id="group">
 
                            <h4 class="subtitle">SEM images that show nanoparticles in daily products (ex: toothpaste and sunscreen)</h4>
 
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                    <div class="row" id="fund">
 
                        <h1 class="section-title col-lg-12">Fundraising and Donation</h1>
 
 
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                     <div class="row">
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                            We held multiple fundraising sales, selling small ice cream dots (resembling nanoparticles!) and Oreo fudge during our lunch periods in school, and making “glitter slime” at our school’s annual spring fair (see Spring Fair in the Outreach section above). (Team activity)
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                             <img src="https://static.igem.org/mediawiki/2017/b/b3/T--TAS_Taipei--Shower_Head-min.jpg" alt="test" id="group">
                            <br><br>In total, we raised around 500 USD, and donated the money to two organizations:
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                            <h4 class="subtitle">Product of Apex Nanotek: Silver Spring Shower Head.<span class="subCred"></span></h4>
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                             <img src="https://static.igem.org/mediawiki/2017/6/6a/T--TAS_Taipei--WaterIsLife.png" alt="test" id="group">
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                             <img src="https://static.igem.org/mediawiki/2017/1/1e/T--TAS_Taipei--SEM_comparison_Tap_vs._Showerhead.png" alt="test" id="group2">
                         <h4 class="para col-lg-12">
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                            <h4 class="subtitle"><b>Figure 1-3 Tap water under SEM.</b><span class="subCred"></span></h4>
                            WaterisLife is an organization that provides clean drinking water, as well as sanitation and hygiene education programs to schools and communities in need. We donated to this organization in hopes that more people will have access to clean water. Visit WaterisLife <a href="http://waterislife.com/">here</a>.
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                             <img src="https://static.igem.org/mediawiki/2017/9/96/T--TAS_Taipei--TEPU.gif" alt="test" id="group">
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                         <h4 class="para col-lg-12">
 
                         <h4 class="para col-lg-12">
                             Taiwan Environmental Protection Union (TEPU) is a local organization founded in 1987 to promote public awareness and participation to prevent pollution and damage to public resources. Visit TEPU <a href="http://www.tepu.org.tw/?page_id=4975">here</a>.
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                             The image on the left shows a tap water sample under the SEM, in which we observed some bacteria (round objects that are approximately 1 μm in diameter). The SEM image on the left shows water that was filtered by the showerhead from Apex nanotek. There is less bacteria as the showerhead uses embedded nanosilver antibacterial filters. (SEM images: Christine C. and Florence L.)
 
                         </h4>
 
                         </h4>
 
                     </div>
 
                     </div>
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                     <div class="row" id="Dihua">
                         <h1 class="section-title col-lg-12">Public Outreach -- A Tour of Taipei</h1>
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                         <h1 class="section-title col-lg-12">WWTP -- Dihua Wastewater Treatment Plant</h1>
 
                     </div>
 
                     </div>
 
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                         <h4 class="para col-lg-8">
                             Some members of the iGEM team went to various popular sites in Taipei to pass out fliers and conduct surveys. We visited National Taiwan University, Chiang Kai-Shek Memorial Hall, and Taipei 101. This helped us collect feedback from different age groups and backgrounds. This was a great and fun way to spread awareness of nanoparticle pollution! (Team members: Ashley L., Emily C., Florence L., Candice L., Yvonne W., Justin Y., Avery W., Christine C., Jesse K., and Laurent H.)
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                             In order to learn firsthand about the effect of nanoparticles in WWTPs, we visited the Dihua WWTP (迪化污水處理廠). Here, we were given a tour around the plant, and were able to ask questions to the managers and people that work there. They confirmed that the current facilities are unable to remove nanoparticles from wastewater mainly due to their small size. In addition to this information, they kindly provided us with samples of sludge, effluent water, and the polymers they add during wastewater processing. Throughout the year we visited and talked to the Dihua WWTP several times about where and how our project could be implemented in their current system. These conversations and visits played a huge role in shaping our construct design, prototype design, mathematical modeling and overall purpose for our project. (Whole team activity)
 
                         </h4>
 
                         </h4>
 
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                             <img src="https://static.igem.org/mediawiki/2017/f/f9/T--TAS_Taipei--Survey_Trip_Gran-min.jpg" alt="test" id="group">
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                             <img src="https://static.igem.org/mediawiki/2017/c/ce/T--TAS_Taipei--Dihua_Tank-min.jpg" alt="test" id="group">
 
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                             Here's a video we made for this event.
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                             <img src="https://static.igem.org/mediawiki/2017/e/e1/T--TAS_Taipei--DihuaDiagram-new.jpg" alt="test" id="group">
                        </h4>
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                            <h4 class="subtitle">We plan to add our bacteria either in the deep aeration tanks or the secondary sedimentation tanks. The disinfection tank will kill the bacteria used in previous tanks.<span class="subCred">Figure: Christine C.</span></h4>
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                        </div>
 
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                             <source src="https://static.igem.org/mediawiki/2017/9/97/T--TAS_Taipei--Dihua_WWTP_%281%29.mp4" type="video/mp4"> Your browser does not support the video tag.
 
                         </video>
 
                         </video>
 
                     </div>
 
                     </div>
                     <div class="row" id="survey">
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                     <div class="row" id="Boswell">
                         <h1 class="section-title col-lg-12">Survey Results</h1>
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                         <h1 class="section-title col-lg-12">Boswell Wastewater Treatment Plant</h1>
 
                     </div>
 
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                             We created a survey that helped us identify public knowledge and misconceptions about synthetic biology and nanoparticle usage. Over 240 people completed the survey. (Survey created by Abby H., Christine C. and Emily C.)
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                             Not all WWTPs are as large as the one in Taipei. One of our advisors (Jude Clapper) went to visit the Boswell WWTP in rural southwestern Pennsylvania. We learned that the same processes that occur in the Taipei Dihua WWTP also occur in the Boswell WWTP, but with different water flow rates and waste quantities. Because of the similarities in how both WWTPs process their wastewater, It inspired us to create our current prototype design that is a rotating polymeric bioreactor coated in biofilm. This prototype will be placed in the secondary sedimentation tank, where the majority of organic solids have been removed and only smaller particles exist. The Boswell WWTP also confirmed that since our project is bacteria-based, it will be killed by UV light and chlorine in the disinfection tank, similar to the Dihua WWTP, before the water turns into effluent and goes to the rivers and oceans.
                            <br><br> Here are some results from our survey:
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                            <br><br> <u>General Questions</u>
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                            <br><br>
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                            <ul>
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                                <li>The majority of people think that gene modification is acceptable if the goal is to save or improve quality of life; however, it is not acceptable for non-medical related reasons, such as changing hair or eye color. In addition, most people do not have a preference between chemical or biological drug synthesis. <i>These results suggest that people are accepting of genetic engineering when it is related to health and medicine.</i></li>
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                                <li>Environmentally, people are generally concerned with the wastewater that enters the ocean and the river. <i>This gives weight to our project, because the quality of water is an important concern for the general public.</i></li>
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                            </ul>
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                             <img src="https://static.igem.org/mediawiki/2017/9/97/T--TAS_Taipei--general_questionsPic.JPG" alt="test" id="group">
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                             <img src="https://static.igem.org/mediawiki/2017/2/2f/T--TAS_Taipei--BoswellDiagram-new.jpg" alt="test" id="group">
                             <h4 class="subtitle"><b>Two examples of general questions from our survey.</b> (<b>Left</b>) 87% (201 out of 243 total responses) think that genes should be modified if the goal is to save or improve quality of life. (<b>Right</b>) 96.7% of the people surveyed care about the quality of wastewater (236 out of 244 total responses).<span class="subCred">Figure: Christine C.</span></h4>
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                             <h4 class="subtitle">We plan to add our bacteria either in the deep aeration tanks or the secondary sedimentation tanks. The disinfection tank will kill the bacteria used in previous tanks.<span class="subCred">Figure: Christine C.</span></h4>
 
                         </div>
 
                         </div>
                    </div><br>
 
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                        <h4 class="para col-lg-12">
 
                            <u>Project-Specific Questions</u>
 
                            <ul>
 
                                <li>The majority of people have heard of nanoparticles and know that nanoparticles are used in consumer products; however, they do not know <i>why</i> nanoparticles are used.</li>
 
                                <li>Most people believe that the government and nanoparticle manufacturers should share responsibility for the regulation of nanoparticle usage and disposal.</li>
 
                            </ul>
 
                        </h4>
 
 
                     </div>
 
                     </div>
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                     <div class="row" id="EPA">
                        <div class="image_container col-lg-10 col-lg-offset-1">
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                         <h1 class="section-title col-lg-12">Thomas J. Brown</h1>
                            <img src="https://static.igem.org/mediawiki/2017/9/9e/T--TAS_Taipei--project_specific-new.JPG" alt="test" id="group">
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                            <h4 class="subtitle"><b>Two examples of project-specific questions from our survey.</b> (<b>Left</b>) A majority of the people we asked (58.6%) do not know why nanoparticles are used in consumer products (143 out of 244 total responses). (<b>Right</b>) People believe that nanoparticle manufacturers and the government (including WWTPs) are most responsible for the regulation of nanoparticle usage and disposal.
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                                <span class="subCred">Figure: Christine C.</span></h4>
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                        </div>
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                        <h4 class="para col-lg-4">
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                            Click to see all survey results:
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                        </h4>
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                        <a href="https://static.igem.org/mediawiki/2017/b/b9/T--TAS_Taipei--general_questions.pdf" type="button" class="btn btn-info col-lg-3">
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                            General Questions
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                        </a>
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                        <a href="https://static.igem.org/mediawiki/2017/3/32/T--TAS_Taipei--project_specific.pdf" type="button" class="btn btn-info col-lg-3 col-lg-offset-1">
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                            Project Specific
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                        </a>
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                    </div>
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                    <div class="row" id="policy">
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                         <h1 class="section-title col-lg-12">Policy Brief -- Nanoparticle Regulation Issues and Case Studies</h1>
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                         <h4 class="para col-lg-12">
                             Our team has conducted extensive research on existing regulatory laws and policies regarding nanoparticles and nanomaterials. We have investigated chemical regulations, including the Restriction, Evaluation, Authorization, and Restriction of Chemicals (REACH), A Toxic Substances Control Act (TCSA), CLP, and the Clean Air Act (CAA). There are significant obstacles to successfully regulating nanoparticles, such as conflicting definitions on nanoparticles that lead to an inability to successfully regulate manufacturers. Research has also been conducted on the hazardous effects of nanoparticles on the human body and environment. We decided to compose a policy brief highlighting the existing challenges in nanoparticle regulation and the lessons learned from previous failure to regulate new chemical substances. The brief was sent out to regulatory agencies, government agencies, and news outlets to raise awareness about the issue. We feel responsible to let others know about the damage nanoparticle waste can do to the environment. (Policy Brief created by Ashley L.)
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                             Thomas J. Brown, the Water Program Specialist of the Pennsylvania Department of Environmental Protection (DEP) occasionally helps with the Boswell Wastewater Treatment Plant. He has also worked with the EPA in Taiwan on wastewater treatments. We interviewed Mr. Brown about our methods to clean nanoparticles in wastewater treatment plants and how to achieve our goal of implementation. With his expertise in the field of wastewater treatment, he provided us some suggestions as to how we could turn our project into reality.
 
                         </h4>
 
                         </h4>
                    </div>
 
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                        <a href="https://static.igem.org/mediawiki/2017/0/0c/T--TAS_Taipei--policy_brief_pdf.pdf" type="button" class="btn btn-info col-lg-4 col-lg-offset-4">
 
                            Click here to read our policy brief!
 
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                        <h4 class="para col-lg-9">
 
                            We sent this policy brief to the <b>Environmental Protection Administration (EPA) minister in Taiwan</b>, and they responded! They read our policy brief and said that they will take it into consideration when they make policy regulations on the use of nanoparticles in the future. They understand that nanotechnology is still developing and definitely needs more attention and regulation. (Correspondence: Christine C.)
 
                        </h4>
 
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                            <img src="https://static.igem.org/mediawiki/2017/9/95/T--TAS_Taipei--%E6%9D%8E%E6%87%89%E5%85%83.JPG" alt="test" id="group">
 
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                        <a href="https://static.igem.org/mediawiki/2017/6/6f/T--TAS_Taipei--EPAResponse.pdf" type="button" class="btn btn-info col-lg-4 col-lg-offset-4">
 
                            Click to see his reply!
 
                        </a>
 
 
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                             We were interviewed by <b>News Lens International</b> about nanoparticle regulation. Many of the questions focused on why we chose to target nanoparticles and how nanoparticles are dealt with in Taiwan. We emphasized that the lack of regulatory legislation prevents agencies from acquiring regulatory power. We also talked about the lack of nanoparticle filtration in wastewater treatment plants. (Interviewed by Ashley L.)
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                             For example, we asked him if there were differences between rural and urban plants that we should take into consideration when thinking about implementing our project. He responded that the biological processes used for treatment remains the same regardless of facility size. This helped us think about and design our final prototype, which can potentially be used in both rural and urban treatment plants.
 
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                         <a href="https://international.thenewslens.com/article/80829" type="button" class="btn btn-info col-lg-4 col-lg-offset-4">
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                         <a href="https://static.igem.org/mediawiki/2017/1/15/T--TAS_Taipei--TomBrownResponse.pdf" type="button" class="btn btn-info col-lg-4 col-lg-offset-4">
                             View the article here
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                             Click here to see Tom Brown’s full response
 
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Revision as of 08:22, 18 October 2017

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HP GOLD INTEGRATED

At the beginning of our project, we visited local and foreign wastewater treatment plants and learned that there are currently very few water purification methods that specifically target nanoparticle waste. We designed our constructs, prototype and modeling based directly from information given by these treatment plants. Our biofilm parts collection was designed to be able to control biofilm production in sedimentation tanks. Our proteorhodopsin construct is designed to be used in aeration tanks where other microbes are already breaking down organic substances. We also use the pre-existing biosafety aspect of the wastewater plants. Additionally, we reached out to several nanoparticle manufacturers, researchers, disposal services and wastewater experts who provided us with information on the advantages and potential consequences of nanoparticle usage, as well as where to target cleaning nanoparticle waste. The feedback we received guided the direction of our project and confirmed the importance of our project to our community.

Bioethics Panel

We hosted a Bioethics Panel, where we invited students and teachers to discuss the moral, social and environmental concerns of our project. To encourage participants to consider the problems from multiple perspectives, we created a role-playing game and assigned different roles to participants. We then asked for their opinions on nanoparticle usage and disposal from the perspective of their assigned role. (Whole team activity)

For instance, one of our questions was:

“Dihua WWTP has no nanoparticle removal plan. Should this be the job of the wastewater plant? Or the nanoparticle manufacturer?”

The following roles were assigned:
  • Wastewater plant manager
  • Nanoparticle manufacturer
  • Citizen
  • Fisherman
  • Fish

Most of the wastewater plant managers thought that nanoparticle manufacturers should be responsible for removing nanoparticles, because they have more information (e.g., solubility, toxicity, etc.) about their own products. However, many other participants were skeptical that manufacturers could be trusted to remove their own contamination and agreed that WWTPs should ultimately be responsible for cleaning water contaminated with nanoparticles.
This activity gave us great insight on how the public perceives nanoparticle usage and regulation in society. This also gave us a chance to talk to people about both the benefits and the dangers of using nanoparticles.
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Apex Nanotek

To learn more about the applications of nanoparticles, we visited a nanotech company that uses silver nanoparticles to make various antimicrobial products. The researcher and manager of Apex Nanotek, Chery Yang, introduced us to their main product, which is antimicrobial nanosilver activated carbon. Pure activated carbon, commonly used to treat sewage and industrial exhaust, is prone to bacterial growth. To overcome this problem, they integrate crystallized nanosilver into the activated carbon for their antimicrobial effects. One of their products is a showerhead, with nanosilver activated carbon filters to kill bacteria when water flows through the showerhead.

We tested the product by comparing SEM images between tap water and filtered water from the showerhead. The showerhead decreased the number of bacteria and larger particles from tap water! However, we also observed the release of nanoparticles from the filter, which will flow into wastewater. (Interviewed by Christine C., Kelly C., Yvonne W., Chansie Y., and Justin Y.)

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Chery Yang (third person from the left), the main researcher of Apex Nanotek Corporation

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Product of Apex Nanotek: Silver Spring Shower Head.

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Figure 1-3 Tap water under SEM.


The image on the left shows a tap water sample under the SEM, in which we observed some bacteria (round objects that are approximately 1 μm in diameter). The SEM image on the left shows water that was filtered by the showerhead from Apex nanotek. There is less bacteria as the showerhead uses embedded nanosilver antibacterial filters. (SEM images: Christine C. and Florence L.)

WWTP -- Dihua Wastewater Treatment Plant

In order to learn firsthand about the effect of nanoparticles in WWTPs, we visited the Dihua WWTP (迪化污水處理廠). Here, we were given a tour around the plant, and were able to ask questions to the managers and people that work there. They confirmed that the current facilities are unable to remove nanoparticles from wastewater mainly due to their small size. In addition to this information, they kindly provided us with samples of sludge, effluent water, and the polymers they add during wastewater processing. Throughout the year we visited and talked to the Dihua WWTP several times about where and how our project could be implemented in their current system. These conversations and visits played a huge role in shaping our construct design, prototype design, mathematical modeling and overall purpose for our project. (Whole team activity)

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We plan to add our bacteria either in the deep aeration tanks or the secondary sedimentation tanks. The disinfection tank will kill the bacteria used in previous tanks.Figure: Christine C.


Boswell Wastewater Treatment Plant

Not all WWTPs are as large as the one in Taipei. One of our advisors (Jude Clapper) went to visit the Boswell WWTP in rural southwestern Pennsylvania. We learned that the same processes that occur in the Taipei Dihua WWTP also occur in the Boswell WWTP, but with different water flow rates and waste quantities. Because of the similarities in how both WWTPs process their wastewater, It inspired us to create our current prototype design that is a rotating polymeric bioreactor coated in biofilm. This prototype will be placed in the secondary sedimentation tank, where the majority of organic solids have been removed and only smaller particles exist. The Boswell WWTP also confirmed that since our project is bacteria-based, it will be killed by UV light and chlorine in the disinfection tank, similar to the Dihua WWTP, before the water turns into effluent and goes to the rivers and oceans.

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We plan to add our bacteria either in the deep aeration tanks or the secondary sedimentation tanks. The disinfection tank will kill the bacteria used in previous tanks.Figure: Christine C.

Thomas J. Brown

Thomas J. Brown, the Water Program Specialist of the Pennsylvania Department of Environmental Protection (DEP) occasionally helps with the Boswell Wastewater Treatment Plant. He has also worked with the EPA in Taiwan on wastewater treatments. We interviewed Mr. Brown about our methods to clean nanoparticles in wastewater treatment plants and how to achieve our goal of implementation. With his expertise in the field of wastewater treatment, he provided us some suggestions as to how we could turn our project into reality.

For example, we asked him if there were differences between rural and urban plants that we should take into consideration when thinking about implementing our project. He responded that the biological processes used for treatment remains the same regardless of facility size. This helped us think about and design our final prototype, which can potentially be used in both rural and urban treatment plants.