Difference between revisions of "Team:NPU-China"
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Revision as of 15:16, 18 October 2017
Abstract
Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent
polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average
annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion
US dollars. It has broad market prospect. At present, acrylic acid is made from propylene (which
is obtained by petroleum cracking) after multi-step treatment. The production process causes pollution,
high energy consumption and it is unsustainable.
This year, we aim to use a green and environmentally
friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional
chemical synthesis methods, Synbio is green and sustainable, and glycerol is cheaper than ethylene.
We construct cell factory based on 4 levels, which are—
Core Part
We use CEAS2 enzyme as the core part, but acrylic acid is a byproduct of CEAS2 enzyme, the wild type 's catalytic effect is very weak, whose production is only 1mg/L. So we hope to improve the catalytic effect of CEAS2 enzyme.
We designed Ceas2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) platform and screened for better-worked Ceas2 mutants by HPLC(High Performance Liquid Chromatography) and HTS(High throughput screening).
Project Three
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Project Four
All of the previous processes are to build the engineering strains which have a high production of acrylic acid that we need. In the subsequent fermentation, we also need to determine the best parameters of the engineering strain.
Therefore, we selected the carbon source, Buffer, temperature, pH and other conditions to optimize the cell production process.
