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<h4>We need to design two different metabolic pathways for two different chassis organisms and propose different optimization schemes for them.We introduced the CEAS2 enzyme exogenously on the basis of the glycerol metabolism of the two bacteria, so that it could produce the target product acrylic acid using the intermediates G3P and DHAP.Besides having finished the construction of the pathways, we also reconstructed and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway and reduce the loss of bypass carbon flux.</h4> | <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different optimization schemes for them.We introduced the CEAS2 enzyme exogenously on the basis of the glycerol metabolism of the two bacteria, so that it could produce the target product acrylic acid using the intermediates G3P and DHAP.Besides having finished the construction of the pathways, we also reconstructed and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway and reduce the loss of bypass carbon flux.</h4> |
Revision as of 15:46, 18 October 2017