Difference between revisions of "Team:Cardiff Wales/Team Luc"

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<p align="left" style="background-color:#ffffff;"> <br><br><br>Team Luciferase consisted of Tom and Jade. Their objective was to isolate various level 0 parts that would be used in gene constructs, and to measure the gene expression of two standard promoters. These are the constitutive 35S promoter, and the inducible LexA promoter. <br><br><br><br><br><br></p>
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<p align="left" style="background-color:#ffffff;"> <br><br><br>Team Luciferase consisted of Tom and Jade. Their objective was to isolate various level 0 parts that would be used in gene constructs, and to measure the gene expression of two standard promoters. These are the constitutive 35S promoter, and the inducible LexA promoter. Team Luciferase successfully prepared level 0 parts (NosT, 35S, LexA, GFP, XVE) from stocks and transformed various parts from the iGEM registry. The team were solely responsible for making and testing the viability of competent E.coli, which were to be used by the other sub teams, as well as the making of protein gels for general use. The team was involved in the preparation and infiltration of <i> Agrobacterium </i> into <i> Nicotiana benthamiana </i>. One construct that was infiltrated was the 35S-Luc+-NosT construct. A luciferin assay experiment was designed with the assistance of our supervisor to test the 35S-Luc+-NosT construct. This involved testing the effect of different concentrations of luciferin on agro/construct infiltrated leaf disc samples. This can be seen on the <a href="https://2017.igem.org/Team:Cardiff_Wales/results"> results page</a>. Tom was also chief liquid nitrogen lad.<br><br><br><br><br><br></p>
  
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Revision as of 14:33, 20 October 2017




Team Luciferase









Team Luciferase consisted of Tom and Jade. Their objective was to isolate various level 0 parts that would be used in gene constructs, and to measure the gene expression of two standard promoters. These are the constitutive 35S promoter, and the inducible LexA promoter. Team Luciferase successfully prepared level 0 parts (NosT, 35S, LexA, GFP, XVE) from stocks and transformed various parts from the iGEM registry. The team were solely responsible for making and testing the viability of competent E.coli, which were to be used by the other sub teams, as well as the making of protein gels for general use. The team was involved in the preparation and infiltration of Agrobacterium into Nicotiana benthamiana . One construct that was infiltrated was the 35S-Luc+-NosT construct. A luciferin assay experiment was designed with the assistance of our supervisor to test the 35S-Luc+-NosT construct. This involved testing the effect of different concentrations of luciferin on agro/construct infiltrated leaf disc samples. This can be seen on the results page. Tom was also chief liquid nitrogen lad.