<p align="left" style="background-color:#ffffff;"> <br><br><br>Team TSH consisted of Emily, Helen and Sarah. The objective of this team was to isolate the thyroid stimulating hormone antagonist itself in two different forms. These were with 'His tags' and without. Both of these constructs can be found on the <a href="https://2017.igem.org/Team:Cardiff_Wales/basicparts"> basic parts page</a>. This team was responsible for creating the <a href="https://2017.igem.org/Team:Cardiff_Wales/compositeparts"> level one constructs</a> with the 35S or LexA promoter containing the TSH or TSHH CDS. The <a href="https://2017.igem.org/Team:Cardiff_Wales/basicparts"> level zero constructs</a> were put together using the type IIS restriction enzyme BsmB1 at 55 degrees celcius (Not 37!). These level 0 parts were then combined to create the constructs mentioned above using Bsa1 which (thankfully) does cut at 37 degrees. These constructs were used to transform <i> Agrobacterium </i>, which was infiltrated into our <i> Nicotiana benthamiana </i> plants. Unfortunately however, protein extractions from these plants were unable to definitively show that the TSH antagonist was produced, but we know the construct works as shown by the luciferase reporter gene work performed by <a href="https://2017.igem.org/Team:Cardiff_Wales/Team_Luc"> team luciferase</a>! We ran out of time in the lab to carry out these assays, but perhaps future attempts may prove more successful!