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<p> After spinning down the culture in flasks, the cells were resuspended in 1x PBS. The OD<sub>600</sub> readings were taken and are shown as follows: | <p> After spinning down the culture in flasks, the cells were resuspended in 1x PBS. The OD<sub>600</sub> readings were taken and are shown as follows: | ||
− | + | <table> | |
+ | <tr> | ||
+ | <th>Condition</th> | ||
+ | <th> OD<sub>600</sub> of replicate 1</th> | ||
+ | <th> OD<sub>600</sub> of replicate 2</th> | ||
+ | <th> OD<sub>600</sub> of replicate 3</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>pET29b in BL21 (Negative control)</td> | ||
+ | <td>2.659</td> | ||
+ | <td>2.001</td> | ||
+ | <td>2.899</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Glucose (Positive control)</td> | ||
+ | <td>1.934</td> | ||
+ | <td>1.887</td> | ||
+ | <td>1.919</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Pure VFAs</td> | ||
+ | <td>0.510</td> | ||
+ | <td>0.571</td> | ||
+ | <td>0.532</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Fermented "syn poo" supernatant</td> | ||
+ | <td>2.533</td> | ||
+ | <td>2.559</td> | ||
+ | <td>2.349</td> | ||
+ | </tr> | ||
+ | </table> | ||
</p> | </p> | ||
Revision as of 04:59, 21 October 2017
Beta Oxidation
Insert beta-oxidation pathway only image here
Aim
...bla bla bla
Volatile fatty acids & beta-oxidation pathway
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Gene construct
Insert gene construct image here
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Chassis and vector
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Experiment
After spinning down the culture in flasks, the cells were resuspended in 1x PBS. The OD600 readings were taken and are shown as follows:
Condition | OD600 of replicate 1 | OD600 of replicate 2 | OD600 of replicate 3 |
---|---|---|---|
pET29b in BL21 (Negative control) | 2.659 | 2.001 | 2.899 |
Glucose (Positive control) | 1.934 | 1.887 | 1.919 |
Pure VFAs | 0.510 | 0.571 | 0.532 |
Fermented "syn poo" supernatant | 2.533 | 2.559 | 2.349 |