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To reduce manpower, we wrote a program to automatically generate toehold switch sequences from target RNA in put. Then we developed it to a website for the convenience of other iGEMers who are interested in toehold switch application. Though this website can execute many functions to reduce repetitive works for user, careful examination of thermodynamic parameters and RNA secondary structures is still encouraged to get a promising switch sequence. Suboptimal structure analysis is particularly recommended. This function is not included in the main program because it’s too time consuming. However, iGEMers could get access to a professional website with suboptimal structure calculation function through our link under “Model” tab. To help iGEMers get started, we would like to do a brief introduction on our website here. | To reduce manpower, we wrote a program to automatically generate toehold switch sequences from target RNA in put. Then we developed it to a website for the convenience of other iGEMers who are interested in toehold switch application. Though this website can execute many functions to reduce repetitive works for user, careful examination of thermodynamic parameters and RNA secondary structures is still encouraged to get a promising switch sequence. Suboptimal structure analysis is particularly recommended. This function is not included in the main program because it’s too time consuming. However, iGEMers could get access to a professional website with suboptimal structure calculation function through our link under “Model” tab. To help iGEMers get started, we would like to do a brief introduction on our website here. | ||
<h3>Create Your Account</h3> | <h3>Create Your Account</h3> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/de/CUHK_register.png" width="20%" height="auto"> | ||
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An account is required for every user to execute the main program, save the result and retrieve result later. A total of fifty results could be saved in one account. | An account is required for every user to execute the main program, save the result and retrieve result later. A total of fifty results could be saved in one account. | ||
<br> | <br> | ||
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<h3>Output excel file</h3> | <h3>Output excel file</h3> | ||
The output result will be represented in a excel file. | The output result will be represented in a excel file. | ||
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+ | <img src="https://static.igem.org/mediawiki/2017/f/fa/CUHK_Excel.png" width="100%" height="auto"> | ||
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Revision as of 12:52, 21 October 2017