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<div id="apDiv15">6.Take out 200ul cultures obtained from Procedure 5 and measure the fluorescent intensity and the amount of bacteria using flowcytometry after 15h. <br> | <div id="apDiv15">6.Take out 200ul cultures obtained from Procedure 5 and measure the fluorescent intensity and the amount of bacteria using flowcytometry after 15h. <br> | ||
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− | <div id="apDiv16"><img src="form2.jpg" alt="<img src="form2.jpg" width="866" height="301" />" width="618" height="302" /> <img src="begger.png" width="344" height="202"> </div> | + | <div id="apDiv16"><img src="https://static.igem.org/mediawiki/2017/6/62/Tsinghua-a-killing-form2.jpg" alt="<img src="form2.jpg" width="866" height="301" />" width="618" height="302" /> <img src="https://static.igem.org/mediawiki/2017/7/79/Tsinghua-a-killing-begger.png" width="344" height="202"> </div> |
B.Test if the killing ability is adequate <br> | B.Test if the killing ability is adequate <br> | ||
5. Construct the following 8 groups and then incubate the cultures at 37℃, shaking at 220rpm. For each group, 3 replicates are constructed, numbered from 1 to 3. Each replicate contains the bacteria with the same number. All the medium contain 100ug/ml chloramphenicol. </div> | 5. Construct the following 8 groups and then incubate the cultures at 37℃, shaking at 220rpm. For each group, 3 replicates are constructed, numbered from 1 to 3. Each replicate contains the bacteria with the same number. All the medium contain 100ug/ml chloramphenicol. </div> | ||
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1.warrior 1 kills itself but warrior doesn’t .We compare the amount of warriors in medium containing A+K with the amount of warriors in medium containing C. If the amount is apparently lower in the medium containing C, the warrior must have killed itself.We find that the amount of warrior 1 is apparently lower in C group, which means that warrior 1 kills itself. Figure1 shows the result. The ratio represents the amount of warriors in C medium divided by in A+K medium.(group4/group1,group 5/group 2) *A+K stands for ampicilin+kanamycin,C stands for chloramphenicol. </div> | 1.warrior 1 kills itself but warrior doesn’t .We compare the amount of warriors in medium containing A+K with the amount of warriors in medium containing C. If the amount is apparently lower in the medium containing C, the warrior must have killed itself.We find that the amount of warrior 1 is apparently lower in C group, which means that warrior 1 kills itself. Figure1 shows the result. The ratio represents the amount of warriors in C medium divided by in A+K medium.(group4/group1,group 5/group 2) *A+K stands for ampicilin+kanamycin,C stands for chloramphenicol. </div> | ||
− | <div id="apDiv20"><img src="fig1. | + | <div id="apDiv20"><img src="https://static.igem.org/mediawiki/2017/5/5a/Tsinghua-a-killing-fig1.jpg" width="301" height="233"><br> |
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In contrast with the result above, we find that when we mix beggar1 and warrior 2 together, the amount of warrior2 surely surpasses that of beggar1. Besides, the killing ability of warrior2 is adequate. The killing ability is neither too strong when the ratio is 1:1, nor is it too weak when the ratio is 1:10. <br> | In contrast with the result above, we find that when we mix beggar1 and warrior 2 together, the amount of warrior2 surely surpasses that of beggar1. Besides, the killing ability of warrior2 is adequate. The killing ability is neither too strong when the ratio is 1:1, nor is it too weak when the ratio is 1:10. <br> | ||
Furthermore, we compared the amount of beggars when they are cultured alone with the amount of beggars when they are cultured with warriors. We calculated the amount of beggars in group 7/group 8 to see the change in beggar1 and in group11/group12 to see the change in beggar2.From figure 4, we can see that the amount of beggar1 drops drastically when it is cultured with warrior2, which means that warrior2 really work. As the amount of beggar2 does not have an apparent change, we may say that warrior1’s killing ability is surely too weak. </div> | Furthermore, we compared the amount of beggars when they are cultured alone with the amount of beggars when they are cultured with warriors. We calculated the amount of beggars in group 7/group 8 to see the change in beggar1 and in group11/group12 to see the change in beggar2.From figure 4, we can see that the amount of beggar1 drops drastically when it is cultured with warrior2, which means that warrior2 really work. As the amount of beggar2 does not have an apparent change, we may say that warrior1’s killing ability is surely too weak. </div> | ||
− | <div id="apDiv25"><img width="256" height="172" src=" | + | <div id="apDiv25"><img width="256" height="172" src="https://static.igem.org/mediawiki/2017/b/b9/Tsinghua-a-killing-fig2.jpg" align="left" hspace="12" alt="w2_b1"><img width="246" height="167" src="paiban_clip_image004_0001.gif" align="left" hspace="12" alt="w1_b2"><br> |
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Revision as of 14:30, 21 October 2017