Difference between revisions of "Team:WashU StLouis/Notebook"
| Line 525: | Line 525: | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
| − | <li> Prepared LB Media <em> | + | <li> Prepared LB Media <em>[Micah, Maddie, Alex]</em></li> |
| − | <li> Re-suspended DNA (<em>D. Radiodurans</em> Uracil DNA Glycosylate 2) from iGEM kit <em> | + | <li> Re-suspended DNA (<em>D. Radiodurans</em> Uracil DNA Glycosylate 2) from iGEM kit <em>[Micah, Maddie, Alex]</em></li> |
| − | <li> Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation) <em> | + | <li> Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation) <em>[Micah, Maddie, Alex]</em></li> |
| − | <li> Prepared LB Agar <em> | + | <li> Prepared LB Agar <em>[Micah, Maddie, Alex]</em></li> |
| − | <li> Prepared overnight culture to make competent cells (MHD42) <em> | + | <li> Prepared overnight culture to make competent cells (MHD42) <em>[Micah, Maddie, Alex]</em></li> |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
| Line 538: | Line 538: | ||
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
| − | <p><em>Micah, Maddie</em></p> | + | <p><em>Present: Micah, Maddie</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
| − | <li> Prepared MHD42 competent cells <em> | + | <li> Prepared MHD42 competent cells <em>[Micah, Maddie]</em></li> |
| − | <li> Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2 <em> | + | <li> Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2 <em>[Micah, Maddie]</em></li> |
<ul> | <ul> | ||
<li> Transformation was a success with 100 colonies </li> | <li> Transformation was a success with 100 colonies </li> | ||
<li> Plate was stored in 4 deg. Celsius for future use </li> | <li> Plate was stored in 4 deg. Celsius for future use </li> | ||
</ul> | </ul> | ||
| − | <li> Prepared TSS Buffer <em> | + | <li> Prepared TSS Buffer <em>[Micah, Maddie]</em></li> |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
| Line 570: | Line 570: | ||
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
| − | <p><em>Micah, Maddie, Alex, Mark, Collin</em></p> | + | <p><em>Present: Micah, Maddie, Alex, Mark, Collin</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
| − | <li> Prepared LB + Chloramphenicol (CM) plates <em> | + | <li> Prepared LB + Chloramphenicol (CM) plates <em>[Micah, Maddie, Mark, Collin]</em></li> |
| − | <li> Prepared Ampicillin solution to be used for plates in the future <em> | + | <li> Prepared Ampicillin solution to be used for plates in the future <em>[Micah, Maddie, Mark, Collin]</em></li> |
| − | <li> Made more LB Agar <em> | + | <li> Made more LB Agar <em>[Alex]</em></li> |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
<ol> | <ol> | ||
| − | <li> Discussed shifting focus from Dsup gene to a variety of UV radiation resistance gene <em> | + | <li> Discussed shifting focus from Dsup gene to a variety of UV radiation resistance gene <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> |
| − | <li> Found more genes to compare to Dsup (i.e. phrA in tardigrades and cyanobacteria) <em> | + | <li> Found more genes to compare to Dsup (i.e. phrA in tardigrades and cyanobacteria) <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> |
| − | <li> Met with Dr. Brennan to discuss updates <em> | + | <li> Met with Dr. Brennan to discuss updates <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> |
</ol> | </ol> | ||
</div> | </div> | ||
| Line 589: | Line 589: | ||
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
| − | <p><em>Micah, Maddie, Alex, Mark, Collin</em></p> | + | <p><em>Present: Micah, Maddie, Alex, Mark, Collin</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
| − | <li> Prepared DH5α competent cells <em> | + | <li> Prepared DH5α competent cells <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> |
| − | <li> Tested MHD42 cells for competency <em> | + | <li> Tested MHD42 cells for competency <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> |
| − | <li> Tested DH5α cells for competency <em> | + | <li> Tested DH5α cells for competency <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
<ol> | <ol> | ||
| − | <li> Fleshed out project and decided specific parts to transform [BBa_B0034 (RBS), BBa_R0010 (lac promoter), and BBa_K1499200 (uvsE gene)] into MHD42 cells during the next day <em> | + | <li> Fleshed out project and decided specific parts to transform [BBa_B0034 (RBS), BBa_R0010 (lac promoter), and BBa_K1499200 (uvsE gene)] into MHD42 cells during the next day <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> |
<ul> | <ul> | ||
<li> The <em>Deinococcus radiodurans</em> UV DNA damage endonuclease (uvsE) gene is a UV radiation damage repair gene from the Stanford-Brown-Spelman 2014 team. We plan to use this to compare to other UV radiation repair genes. </li> | <li> The <em>Deinococcus radiodurans</em> UV DNA damage endonuclease (uvsE) gene is a UV radiation damage repair gene from the Stanford-Brown-Spelman 2014 team. We plan to use this to compare to other UV radiation repair genes. </li> | ||
</ul> | </ul> | ||
| − | <li> Planned out more long-term goals such as collaborations with other teams (especially those in the Midwest) <em> | + | <li> Planned out more long-term goals such as collaborations with other teams (especially those in the Midwest) <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> |
</ol> | </ol> | ||
</div> | </div> | ||
| Line 613: | Line 613: | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
| − | <li> Checked competency test for MHD42 cells <em> | + | <li> Checked competency test for MHD42 cells <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> |
<ul> | <ul> | ||
<li> Test was successful! </li> | <li> Test was successful! </li> | ||
<li> <img src="https://static.igem.org/mediawiki/2017/0/0c/T--WashU_StLouis--2017-06-07_MHD42_Comp_Cells_Test_Results.jpg"/></li> | <li> <img src="https://static.igem.org/mediawiki/2017/0/0c/T--WashU_StLouis--2017-06-07_MHD42_Comp_Cells_Test_Results.jpg"/></li> | ||
</ul> | </ul> | ||
| − | <li> Checked competency test for DH5α <em> | + | <li> Checked competency test for DH5α <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> |
<ul> | <ul> | ||
<li> Test showed that the cells were competent but had a low transformation efficiency </li> | <li> Test showed that the cells were competent but had a low transformation efficiency </li> | ||
<li> <img src="https://static.igem.org/mediawiki/2017/a/ae/T--WashU_StLouis--2017-06-07_DH5alpha_Comp_Cells_Test_Results.jpg"/> </li> | <li> <img src="https://static.igem.org/mediawiki/2017/a/ae/T--WashU_StLouis--2017-06-07_DH5alpha_Comp_Cells_Test_Results.jpg"/> </li> | ||
</ul> | </ul> | ||
| − | <li> Prepared LB + Ampicillin (Amp) plates <em> | + | <li> Prepared LB + Ampicillin (Amp) plates <em>[Micah, Mark, Collin]</em></li> |
| − | <li> Resuspeded parts BBa_B0034, BBa_R0010, and BBa_K1499200 from current and past distribution kits <em> | + | <li> Resuspeded parts BBa_B0034, BBa_R0010, and BBa_K1499200 from current and past distribution kits <em>[Maddie, Alex]</em></li> |
| − | <li> Transformed part BBa_B0034 (with plasmid psB1A2) into MHD42 competent cells <em> | + | <li> Transformed part BBa_B0034 (with plasmid psB1A2) into MHD42 competent cells <em>[Maddie, Alex]</em></li> |
| − | <li> Transformed part BBa_R0010 (with plasmid psB1C3) into MHD42 competent cells <em> | + | <li> Transformed part BBa_R0010 (with plasmid psB1C3) into MHD42 competent cells <em>[Maddie, Alex]</em></li> |
| − | <li> Transformed part BBa_K1499200 (with plasmid psB1C3) into MHD42 competent cells <em> | + | <li> Transformed part BBa_K1499200 (with plasmid psB1C3) into MHD42 competent cells <em>[Maddie, Alex]</em></li> |
| − | <li> Prepared overnight culture of DH5α competent cells <em> | + | <li> Prepared overnight culture of DH5α competent cells <em>[Mark, Collin]</em></li> |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
<ol> | <ol> | ||
| − | <li> Discussed modelling ideas <em> | + | <li> Discussed modelling ideas <em>[Micah, Maddie, Mark, Collin]</em></li> |
| − | <li> Learned how to design primers from Eugene <em> | + | <li> Learned how to design primers from Eugene <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> |
</ol> | </ol> | ||
</div> | </div> | ||
Revision as of 05:14, 8 June 2017
Notebook
| June 2017 | ||||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | ||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| 11 | 12 | 13 | 14 | 15 | 16 | 17 |
| 18 | 19 | 20 | 21 | 22 | 23 | 24 |
| 25 | 26 | 27 | 28 | 29 | 30 | |
Present: Micah, Maddie, Alex
Lab Work
- Prepared LB Media [Micah, Maddie, Alex]
- Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit [Micah, Maddie, Alex]
- Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation) [Micah, Maddie, Alex]
- Prepared LB Agar [Micah, Maddie, Alex]
- Prepared overnight culture to make competent cells (MHD42) [Micah, Maddie, Alex]
Outside Work / Discussion
Present: Micah, Maddie
Lab Work
- Prepared MHD42 competent cells [Micah, Maddie]
- Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2 [Micah, Maddie]
- Transformation was a success with 100 colonies
- Plate was stored in 4 deg. Celsius for future use
- Prepared TSS Buffer [Micah, Maddie]
Outside Work / Discussion
No Work!
No Work!
Present: Micah, Maddie, Alex, Mark, Collin
Lab Work
- Prepared LB + Chloramphenicol (CM) plates [Micah, Maddie, Mark, Collin]
- Prepared Ampicillin solution to be used for plates in the future [Micah, Maddie, Mark, Collin]
- Made more LB Agar [Alex]
Outside Work / Discussion
- Discussed shifting focus from Dsup gene to a variety of UV radiation resistance gene [Micah, Maddie, Mark, Collin, Alex]
- Found more genes to compare to Dsup (i.e. phrA in tardigrades and cyanobacteria) [Micah, Maddie, Mark, Collin, Alex]
- Met with Dr. Brennan to discuss updates [Micah, Maddie, Mark, Collin, Alex]
Present: Micah, Maddie, Alex, Mark, Collin
Lab Work
- Prepared DH5α competent cells [Micah, Maddie, Mark, Collin, Alex]
- Tested MHD42 cells for competency [Micah, Maddie, Mark, Collin, Alex]
- Tested DH5α cells for competency [Micah, Maddie, Mark, Collin, Alex]
Outside Work / Discussion
- Fleshed out project and decided specific parts to transform [BBa_B0034 (RBS), BBa_R0010 (lac promoter), and BBa_K1499200 (uvsE gene)] into MHD42 cells during the next day [Micah, Maddie, Mark, Collin, Alex]
- The Deinococcus radiodurans UV DNA damage endonuclease (uvsE) gene is a UV radiation damage repair gene from the Stanford-Brown-Spelman 2014 team. We plan to use this to compare to other UV radiation repair genes.
- Planned out more long-term goals such as collaborations with other teams (especially those in the Midwest) [Micah, Maddie, Mark, Collin, Alex]
Lab Work
- Checked competency test for MHD42 cells [Micah, Maddie, Mark, Collin, Alex]
- Test was successful!
-
- Checked competency test for DH5α [Micah, Maddie, Mark, Collin, Alex]
- Test showed that the cells were competent but had a low transformation efficiency
-
- Prepared LB + Ampicillin (Amp) plates [Micah, Mark, Collin]
- Resuspeded parts BBa_B0034, BBa_R0010, and BBa_K1499200 from current and past distribution kits [Maddie, Alex]
- Transformed part BBa_B0034 (with plasmid psB1A2) into MHD42 competent cells [Maddie, Alex]
- Transformed part BBa_R0010 (with plasmid psB1C3) into MHD42 competent cells [Maddie, Alex]
- Transformed part BBa_K1499200 (with plasmid psB1C3) into MHD42 competent cells [Maddie, Alex]
- Prepared overnight culture of DH5α competent cells [Mark, Collin]
Outside Work / Discussion
- Discussed modelling ideas [Micah, Maddie, Mark, Collin]
- Learned how to design primers from Eugene [Micah, Maddie, Mark, Collin, Alex]
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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