Revision as of 11:36, 22 October 2017

Material&Methods

1.Parts
2.Primer list
3.Materials

3-1. Kit
3-2. Restricton Enzyme
3-3. Polymerase
3-4. DNA ligase
3-5. Marker
3-6. Organism
3-7. Antibiotics
3-8. Equipment
3-9. Backborns
3-10. Buffer
3-11. SDSPAGE/Western blotting

4.Methods

4-1. Miniprep
4-2. Gel Extraction
4-3. Restrict Enzyme Digestion
4-4. Ligation
4-5. Transformation
4-6. PCR
4-7. Sequencing
4-8. RT-PCR
4-10. Western blotting(Membrane fraction)

1.Parts

2.Primer list

Primer name	Sequence	Length	Tm	GC%	Designer	Manufacturer

3.Materials

3-1.Kit

Name	Supplier

3-2.Restriction Enzyme

Name	Supplier

3-3.Polymerase

Name	Supplier

3-4.DNA Ligase

Name	Supplier

3-5.Marker

Name	Supplier

3-6.Organism

Name	Supplier

3-7.Antibiotics

Name	Supplier

3-8.Equipment

Name	Supplier

3-9.Backbones

Name	Supplier

3-10.Buffer

Name	Supplier

3-11.SDSPAGE/Western blotting

Name	Supplier

@@ Line 30: / Line 30: @@
          <li><a href="https://2017.igem.org/team:Kyoto/Project">Project</a>
              <ul>
-               <li><a href="https://2017.igem.org/Team:Kyoto/Project">Description</a></li>
                 <li><a href="https://2017.igem.org/Team:Kyoto/Results">Results</a></li>
                 <li><a href="https://2017.igem.org/Team:Kyoto/Project_Details">Proof of concept</li>
@@ Line 42: / Line 41: @@
              </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Notebook">Notebook</a></li><li><a href="https://2017.igem.org/Team:Kyoto/integrated">Practice</a>
              <ul>
-               <li><a href="https://2017.igem.org/Team:Kyoto/integrated">Human Practice</a></li>
                 <li><a href="https://2017.igem.org/Team:Kyoto/Collaborations">Collaboration</li>
              </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Team">Team</a>
              <ul>
-               <li><a href="https://2017.igem.org/Team:Kyoto/Team">Members</li>
                 <li><a href="https://2017.igem.org/Team:Kyoto/Attributions">Attributions</li>
              </ul></li><li><a href="https://2017.igem.org/Team:Kyoto/Safety">Safety</a></li>
        </ul>
     </header>
     <h1>Material&amp;Methods</h1>
     <ul class="material">

Difference between revisions of "Team:Kyoto/Experiments"

Revision as of 11:36, 22 October 2017

Material&Methods

1.Parts

2.Primer list

3.Materials

3-1.Kit

3-2.Restriction Enzyme

3-3.Polymerase

3-4.DNA Ligase

3-5.Marker

3-6.Organism

3-7.Antibiotics

3-8.Equipment

3-9.Backbones

3-10.Buffer

3-11.SDSPAGE/Western blotting

4.Methods

4-1.Miniprep

4-2.Gel Extraction

4-3.Restriction Enzyme Digestion

4-4.Ligation

4-5.Transformation

4-6.PCR

4-7.Sequenicing

4-8.RT-PCR

4-9.Basic protcol of Western blotting

4-10.Western blotting(Membrane fraction)