Difference between revisions of "Team:Hong Kong-CUHK/Results"
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Purified mRFP and amajLime was diluted to 10µg/100µl (total 200µl), in triplicates, into different buffers which pH ranges from pH2 to pH12. Volume of mRFP: pH buffer=1:1.8, amajLime : pH buffer =1:4.3. The lines represent the trend of the median fluorescence for the measured pH values. mRFP and amajLime were excited by 584nm and 470nm respectively and emission was measured at 607nm and 515nm respectively. To allow repeatable experiment, we converted the relative fluorescent intensity to an absolute fluorophore concentration by plotting standard curve of the fluorophore using the interlab protocol. We used Rhodamine to measure mRFP; Fluorescein to measure amajLime. The results show that the optimum pH of both chromoprotein ranges from pH6 to pH8. Their fluorescent signal thus will probably not be interfered by the pH of our proposed sample: saliva or NP swab. | Purified mRFP and amajLime was diluted to 10µg/100µl (total 200µl), in triplicates, into different buffers which pH ranges from pH2 to pH12. Volume of mRFP: pH buffer=1:1.8, amajLime : pH buffer =1:4.3. The lines represent the trend of the median fluorescence for the measured pH values. mRFP and amajLime were excited by 584nm and 470nm respectively and emission was measured at 607nm and 515nm respectively. To allow repeatable experiment, we converted the relative fluorescent intensity to an absolute fluorophore concentration by plotting standard curve of the fluorophore using the interlab protocol. We used Rhodamine to measure mRFP; Fluorescein to measure amajLime. The results show that the optimum pH of both chromoprotein ranges from pH6 to pH8. Their fluorescent signal thus will probably not be interfered by the pH of our proposed sample: saliva or NP swab. | ||
Revision as of 12:46, 22 October 2017