Difference between revisions of "Team:BNU-China/Results"
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<h2 id="title1">Microtubule</h2> | <h2 id="title1">Microtubule</h2> | ||
<h3>Plasmid construction</h3> | <h3>Plasmid construction</h3> | ||
| − | < | + | <p>We have accomplished the construction of 6 main parts whose functions are described respectively in previous design page. <a href="https://2017.igem.org/Team:BNU-China/Design#title2">(microtubule module)</a></p> |
<img src="https://static.igem.org/mediawiki/2017/e/e1/T-BNU-China-results1.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/e/e1/T-BNU-China-results1.png" alt="Sorry, the image is not spupported by your browser."> | ||
<h4>figure 1</h4> | <h4>figure 1</h4> | ||
| + | |||
<h3>Protein expression</h3> | <h3>Protein expression</h3> | ||
| − | < | + | <p>These are images recorded by the Fluorescence microscope.The engineered yeasts were induced at 30°C for 20 hours. And from the image, we can notice that our engineered yeasts have successfully expressed our recombinant proteins mCherry-αand mCherry. Moreover, the expression rate of mCherry is almost up to 100%. </p> |
<img src="https://static.igem.org/mediawiki/2017/1/19/T-BNU-China-results2.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/1/19/T-BNU-China-results2.png" alt="Sorry, the image is not spupported by your browser."> | ||
<h4>figure 2 Induced 20h in SG-Ura medium；A,B: recipient strain with empty plasmid</h4> | <h4>figure 2 Induced 20h in SG-Ura medium；A,B: recipient strain with empty plasmid</h4> | ||
| + | |||
| + | <p>The image shows the results of a Western blot analysis carried out with an anti-V5 antibody. | ||
| + | The target proteins are expressed by the engineered yeasts which contain pYCα-alpha, pYCα-beta, pYCα-mCherry， pYCα-mCherry-αtubulin and pYD1-beta, respectively.</p> | ||
<img src="https://static.igem.org/mediawiki/2017/2/25/T-BNU-China-results3.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/2/25/T-BNU-China-results3.png" alt="Sorry, the image is not spupported by your browser."> | ||
<h4>figure 3</h4> | <h4>figure 3</h4> | ||
| + | |||
<img src="https://static.igem.org/mediawiki/2017/9/9f/T-BNU-China-results4.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/9/9f/T-BNU-China-results4.png" alt="Sorry, the image is not spupported by your browser."> | ||
<h4>figure 4</h4> | <h4>figure 4</h4> | ||
Revision as of 14:11, 22 October 2017
Results
Microtubule
Plasmid construction
We have accomplished the construction of 6 main parts whose functions are described respectively in previous design page. (microtubule module)
figure 1
Protein expression
These are images recorded by the Fluorescence microscope.The engineered yeasts were induced at 30°C for 20 hours. And from the image, we can notice that our engineered yeasts have successfully expressed our recombinant proteins mCherry-αand mCherry. Moreover, the expression rate of mCherry is almost up to 100%.
figure 2 Induced 20h in SG-Ura medium；A,B: recipient strain with empty plasmid
The image shows the results of a Western blot analysis carried out with an anti-V5 antibody. The target proteins are expressed by the engineered yeasts which contain pYCα-alpha, pYCα-beta, pYCα-mCherry， pYCα-mCherry-αtubulin and pYD1-beta, respectively.
figure 3
figure 4
Flagellar Filament
Plasmid construction
Protein expression
figure 5
figure 6
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