Difference between revisions of "Team:BNU-China/Results"
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<p>We have accomplished the construction of 6 main parts whose functions are described respectively in previous design page. <a href="https://2017.igem.org/Team:BNU-China/Design#title2">(microtubule module)</a></p> | <p>We have accomplished the construction of 6 main parts whose functions are described respectively in previous design page. <a href="https://2017.igem.org/Team:BNU-China/Design#title2">(microtubule module)</a></p> | ||
<img src="https://static.igem.org/mediawiki/2017/e/e1/T-BNU-China-results1.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/e/e1/T-BNU-China-results1.png" alt="Sorry, the image is not spupported by your browser."> | ||
| − | <h4> | + | <h4>Figure 1 The electrophoresis image of these 6 plasmids.</h4> |
<h3>Protein expression</h3> | <h3>Protein expression</h3> | ||
<p>These are images recorded by the Fluorescence microscope.The engineered yeasts were induced at 30°C for 20 hours. And from the image, we can notice that our engineered yeasts have successfully expressed our recombinant proteins mCherry-αand mCherry. Moreover, the expression rate of mCherry is almost up to 100%. </p> | <p>These are images recorded by the Fluorescence microscope.The engineered yeasts were induced at 30°C for 20 hours. And from the image, we can notice that our engineered yeasts have successfully expressed our recombinant proteins mCherry-αand mCherry. Moreover, the expression rate of mCherry is almost up to 100%. </p> | ||
<img src="https://static.igem.org/mediawiki/2017/1/19/T-BNU-China-results2.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/1/19/T-BNU-China-results2.png" alt="Sorry, the image is not spupported by your browser."> | ||
| − | <h4> | + | <h4>Figure 2 Induced 20h in SG-Ura medium；A,B: recipient strain with empty plasmid</h4> |
<p>The image shows the results of a Western blot analysis carried out with an anti-V5 antibody. | <p>The image shows the results of a Western blot analysis carried out with an anti-V5 antibody. | ||
| − | The target proteins are expressed by the engineered yeasts which contain pYCα-alpha, pYCα-beta, pYCα- | + | The target proteins are expressed by the engineered yeasts which contain pYCα-alpha, pYCα-beta, pYCα-mCherry and pYCα-mCherry-αtubulin, respectively.</p> |
<img src="https://static.igem.org/mediawiki/2017/2/25/T-BNU-China-results3.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/2/25/T-BNU-China-results3.png" alt="Sorry, the image is not spupported by your browser."> | ||
| − | <h4> | + | <h4>Figure 3 The results of a Western blot analysis carried out with an anti-V5 antibody</h4> |
| − | + | <p>Our engineered yeasts, containing pYD1-β tubulin vector, have been proved that it can successfully express exogenous βtubulin by Western blot analysis.</p> | |
<img src="https://static.igem.org/mediawiki/2017/9/9f/T-BNU-China-results4.png" alt="Sorry, the image is not spupported by your browser."> | <img src="https://static.igem.org/mediawiki/2017/9/9f/T-BNU-China-results4.png" alt="Sorry, the image is not spupported by your browser."> | ||
| − | <h4> | + | <h4>Figure 4 The partly results of a Western blot analysis carried out with an anti-V5 antibody</h4> |
<h2 id="title2">Flagellar Filament</h2> | <h2 id="title2">Flagellar Filament</h2> | ||
<h3>Plasmid construction</h3> | <h3>Plasmid construction</h3> | ||
Revision as of 14:20, 22 October 2017
Results
Microtubule
Plasmid construction
We have accomplished the construction of 6 main parts whose functions are described respectively in previous design page. (microtubule module)
Figure 1 The electrophoresis image of these 6 plasmids.
Protein expression
These are images recorded by the Fluorescence microscope.The engineered yeasts were induced at 30°C for 20 hours. And from the image, we can notice that our engineered yeasts have successfully expressed our recombinant proteins mCherry-αand mCherry. Moreover, the expression rate of mCherry is almost up to 100%.
Figure 2 Induced 20h in SG-Ura medium；A,B: recipient strain with empty plasmid
The image shows the results of a Western blot analysis carried out with an anti-V5 antibody. The target proteins are expressed by the engineered yeasts which contain pYCα-alpha, pYCα-beta, pYCα-mCherry and pYCα-mCherry-αtubulin, respectively.
Figure 3 The results of a Western blot analysis carried out with an anti-V5 antibody
Our engineered yeasts, containing pYD1-β tubulin vector, have been proved that it can successfully express exogenous βtubulin by Western blot analysis.
Figure 4 The partly results of a Western blot analysis carried out with an anti-V5 antibody
Flagellar Filament
Plasmid construction
Protein expression
figure 5
figure 6
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