|
|
| Line 1: |
Line 1: |
| − | {{Wageningen_UR/Menuv2}} | + | {{Wageningen_UR}} |
| − | {{Wageningen_UR/StyleCSSv2}}
| + | |
| − | {{Wageningen_UR/MobileMenu}}
| + | |
| − | | + | |
| | <html> | | <html> |
| | | | |
| − | <div class="container-fluid OurContent"> | + | <div class="clear"></div> |
| | | | |
| − | <div class="row">
| |
| − | <div class="col-md-2 hidden-xs Main-Left-Column">
| |
| − | </div>
| |
| | | | |
| − | <div class="col-lg-8">
| |
| | | | |
| − | <div class="Main-Border">
| |
| − | <div class="Main-Center-Content-Column Overview">
| |
| − | <div id="breadcrumb-wrapper">
| |
| − | <ul class="breadcrumb">
| |
| − | <li><a href="https://2017.igem.org/Team:Wageningen_UR">Home</a></li>
| |
| − | <li><a href="https://2017.igem.org/Team:Wageningen_UR/Team">Teams</a></li>
| |
| − | <li>Attributions</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | <!--breadcrumb-wrapper -->
| |
| | | | |
| − | <h1>Attributions</h1>
| + | <div class="column full_size"> |
| | + | <h1>Attributions</h1> |
| | | | |
| − | <p> Mantis consists of several parts that together make up the cohesive system. As such, each team member was responsible for a specific module of the project. In addition, each member also played a specific role within the team, next to their lab (or modeling) work. Of our team, eight persons have performed wet lab work and two persons performed modelling experiments to make Mantis a functioning bacterium. Three main components can be distinguished: the detection, reporter and computational module. | + | <p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster. |
| − | </p>
| + | <br><br> |
| | + | This is a bronze medal requirement. Please see the <a href="https://2017.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p> |
| | | | |
| − | <h2>Detection Module</h2>
| + | </div> |
| − | <p>This part encompasses everything involved in sensing antigens contained within a sample of interest. To start off, <b>Niek</b> generated virus-like particles (VLPs) of Zika, Chikungunya and Mayaro, while <b>Linda</b> made <i>Trypanosoma bruscei</i> antigens. Each of these antigens be used in our affinity body library screening. Here, affinity bodies will be generated against these antigens by <b>Jurre</b>. Now that the respective antigens and affibodies are available, the signal generation comes into play. We considered two methods of signal detection here: <b>Stijn</b> was responsible for the Cpx-based system, while <b>Tom</b> designed the ToxR-based system.
| + | |
| − | </p>
| + | |
| | | | |
| − | <h2>Reporter Module</h2>
| |
| − | <p>The generated signal must be transformed into a readable and quantifiable output. <b>Bart</b> extended the Cpx system to include 2 split halves of YFP, which come together under the influence of CpxR dimerization to generate fluorescence. <b>José</b> will choose the optimal reporter protein candidate out of a selection of split fluorescent and split chromoproteins. Finally, signal amplification is studied by <b>Natalia</b>, which employs a quorum sensing system to lyse cells and start an intensifying signalling cascade.
| |
| − | </p>
| |
| | | | |
| − | <h2>Computational module</h2>
| + | <div class="clear"></div> |
| − | <p>On the modelling side of the project, <b>Sabine</b> worked together with multiple team members to make a functional model of the Cpx system kinetics. <b>Mark</b> took care of the quorum sensing model. Results from both models will be used to improve the systems in the lab.
| + | |
| − | </p>
| + | |
| | | | |
| − | <h2>InterLab Study</h2>
| |
| − | <p>Our team participated in the 2017 edition of the iGEM InterLab Study. These experiments were performed by <b>Stijn</b> and <b>Jurre</b>. Together, they performed the required wet lab work for this, processed the accompanying data and sent it to iGEM HQ.
| |
| | | | |
| − | <h2>Team Roles</h2>
| + | <div class="column half_size"> |
| − | <p> Each person also had a separate function within the team for the duration of iGEM. <b>Niek</b> is the Team Captain and is thus the contact point for other teams, the discussion leader during meetings and much, much more. For organisational issues, he gets help from <b>Natalia</b>, our ever-happy Secretary who took care of reserving all relevant rooms for our meetings, as well as writing down the details of every one of them and sending them to the rest of the team afterwards. To make sure that we had enough money, <b>Bart</ b> and < b> Jurre</b> were part of the Funding group, whose goal it was to net us enough resources to carry out our lab work , travel expenses for conventions and ensure our place at the 2017 Jamboree. Cash flows were carefully watched by our Treasurer <b>Stijn</b>, who safeguarded our finances and guaranteed that we would not drop into the red numbers. The Lab Manager, <b>Tom</b>, took care of equipment/resource orders and kept track of all lab expenses. Our Biosafety Officer <b>José</b> made sure everyone worked responsibly and contributed to the Safety documents required for iGEM. The Human Practices team consisted of <b>Linda</b> and <b>Sabine</b>, who were responsible for every event which involves outreach, as well as the development of the story that accompanies Mantis and establishing contact with scientific experts. Our social media accounts were also managed by them. <b>Mark</ b> was the Wiki Manager and is the reason that you are reading this right now on a beautiful, informative and comprehensive web page. | + | <h5> Why is this page needed? </h5> |
| − | </p>
| + | <p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p> |
| | + | <p> |
| | + | For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p> |
| | + | </div> |
| | | | |
| − | <h2>Supervisors</h2>
| |
| − | <p>Head of the entire WUR iGEM 2017 team was Associate Professor <b>dr. Christian Fleck</b>, who was kindly assisted by Assistant Professor <b>dr. Raymond Staals</b> for the duration of the project. <b>dr. Marta Vazquez Vilar</b>, <b>Wen Wu</b>, <b>Prarthana Mohanraju</b> and <b>Rik van Rosmalen</b> were responsible for supervision of students in the lab, while modelling students fell under the care of <b>dr. Robert Smith</b> and <b>Emma Keizer</b>. Occasionally, <b>Stamatios Damalas</b> would attend a meeting and provide us with help where needed. Special thanks goes out to <b>dr. ir. Gorben Pijlman</b> for setting up a collaboration between our team and the Department of Virology, as well as supervision of the VLP project. Finally, <b>dr. Bob Mulder</b> assisted the Human Practices team in their endeavours.
| |
| − | </p>
| |
| | | | |
| − | <h2>Special Thanks</h2>
| + | <div class="column half_size"> |
| − | <p>Of course we want to thank everyone that supported us, from our anonymous financial sponsors to the professors and experts that advised us. The Laboratories of Microbiology (MIB) and Systems and Synthetic Biology (SSB) also deserve our thanks, for allowing us to work here and all the helpful interactions with the nice people who assisted us. Finally, we would also like to thank the iGEM HQ and all its staff, for organizing this competition and making it possible for everyone to participate in such a wonderful event. Everyone: Thank you for making this possible and for the awesome year!
| + | <h5> What should this page have?</h5> |
| − | </p>
| + | |
| − | </div>
| + | |
| | | | |
| − | </div>
| + | <ul> |
| | + | <li>General Support</li> |
| | + | <li>Project support and advice</li> |
| | + | <li>Fundraising help and advice</li> |
| | + | <li>Lab support</li> |
| | + | <li>Difficult technique support</li> |
| | + | <li>Project advisor support</li> |
| | + | <li>Wiki support</li> |
| | + | <li>Presentation coaching</li> |
| | + | <li>Human Practices support</li> |
| | + | <li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li> |
| | + | </ul> |
| | + | </div> |
| | + | |
| | + | |
| | + | <div class="clear"></div> |
| | | | |
| − | </div>
| + | <div class="column half_size"> |
| − | </div>
| + | |
| − | <div class=" col-md-2 hidden-xs Main-Right-Column" > | + | |
| − | </div>
| + | |
| | | | |
| | + | <div class="highlight"> |
| | + | <h5> Can we base our project on a previous one? </h5> |
| | + | <p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p> |
| | + | </div> |
| | </div> | | </div> |
| | | | |
| | | | |
| | + | <div class="column half_size"> |
| | + | |
| | + | <h5>Inspiration</h5> |
| | + | <p>Take a look at what other teams have done:</p> |
| | + | <ul> |
| | + | <li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li> |
| | + | <li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li> |
| | + | <li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li> |
| | + | <li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li> |
| | + | </ul> |
| | + | |
| | + | </div> |
| | + | |
| | + | <div class="clear"></div> |
| | + | |
| | + | <div class="column half_size"> |
| | + | |
| | + | <h5>Team training and Project start</h5> |
| | + | <p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p> |
| | + | <ul> |
| | + | <li>Does your institution teach an iGEM or synthetic biology course?</li> |
| | + | <li>When did you start this course?</li> |
| | + | <li>Are the syllabus and course materials freely available online?</li> |
| | + | <li>When did you start your brainstorming?</li> |
| | + | <li>When did you start in the lab?</li> |
| | + | <li>When did you start working on your project?</li> |
| | + | |
| | + | </ul> |
| | + | |
| | + | </div> |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | </div> |
| | </html> | | </html> |
| − | {{Wageningen_UR/PageEnd}}
| |
| − | {{Wageningen_UR/MainJSv2}}
| |
Wageningen_UR
Attributions
Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.
This is a bronze medal requirement. Please see the Medals requirements page for more details.
Why is this page needed?
The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.
For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.
What should this page have?
- General Support
- Project support and advice
- Fundraising help and advice
- Lab support
- Difficult technique support
- Project advisor support
- Wiki support
- Presentation coaching
- Human Practices support
- Thanks and acknowledgements for all other people involved in helping make a successful iGEM team
Can we base our project on a previous one?
Yes! You can have a project based on a previous team, or based on someone else's idea, as long as you state this fact very clearly and give credit for the original project.
Inspiration
Take a look at what other teams have done:
Team training and Project start
Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:
- Does your institution teach an iGEM or synthetic biology course?
- When did you start this course?
- Are the syllabus and course materials freely available online?
- When did you start your brainstorming?
- When did you start in the lab?
- When did you start working on your project?
