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| | <td>Takara Bio is the biomedical unit of Takara Holdings Inc. and focuses on revolutionary technologies to improve human health. Takara Bio is composed of three units; the Bioindustry division, the AgriBio division and the Gene Therapy division. Takara Bio Europe has offered us a cloning kit and protein purification kit, both of which were frequently used in our experiments.</td> | | <td>Takara Bio is the biomedical unit of Takara Holdings Inc. and focuses on revolutionary technologies to improve human health. Takara Bio is composed of three units; the Bioindustry division, the AgriBio division and the Gene Therapy division. Takara Bio Europe has offered us a cloning kit and protein purification kit, both of which were frequently used in our experiments.</td> |
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| | <td>NTrans Technologies is a company based in Utrecht, specializing in delivery of content into cells using the iTOP technology which was developed in the Geijsen lab of the Hubrecht Institute. NTrans offered us guidance and assistance with our project by facilitating a lab technician experienced in the work we had to do for our project. We thank them for Clara Martinez’ amazing assistance and patience with our silly questions.</td> | | <td>NTrans Technologies is a company based in Utrecht, specializing in delivery of content into cells using the iTOP technology which was developed in the Geijsen lab of the Hubrecht Institute. NTrans offered us guidance and assistance with our project by facilitating a lab technician experienced in the work we had to do for our project. We thank them for Clara Martinez’ amazing assistance and patience with our silly questions.</td> |
| | <td align="center" valign="middle"><img height="75" src="https://static.igem.org/mediawiki/2017/b/bb/Uuntrans.png"></td> | | <td align="center" valign="middle"><img height="75" src="https://static.igem.org/mediawiki/2017/b/bb/Uuntrans.png"></td> |
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| | <td align="center" valign="middle"><img height="100" src="https://static.igem.org/mediawiki/2017/5/5c/Uuidt.jpg"></td> | | <td align="center" valign="middle"><img height="100" src="https://static.igem.org/mediawiki/2017/5/5c/Uuidt.jpg"></td> |
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| | <div><b>Snapgene is a software company, providing useful software for molecular biology.</b> Snapgene offers fast and reliable software to visualize and design molecular biology experiments such as cloning and PCR experiments. They sponsored us with full team licenses for the Snapgene software, which we have used extensively during our project.</div> | | <div><b>Snapgene is a software company, providing useful software for molecular biology.</b> Snapgene offers fast and reliable software to visualize and design molecular biology experiments such as cloning and PCR experiments. They sponsored us with full team licenses for the Snapgene software, which we have used extensively during our project.</div> |
| | <div><b>eLabjournal is a product by BIO-ITECH BV, a company providing software as a service for research laboratories.</b> Their eLabjournal product offers a simple and effective software environment to keep our lab notes organized and allows easy access wherever and whenever we need it. eLabjournal sponsored us with full team licenses for their software.</div> | | <div><b>eLabjournal is a product by BIO-ITECH BV, a company providing software as a service for research laboratories.</b> Their eLabjournal product offers a simple and effective software environment to keep our lab notes organized and allows easy access wherever and whenever we need it. eLabjournal sponsored us with full team licenses for their software.</div> |
| | + | <div><b>NTrans Technologies specializes in the delivery of content into cells using the iTOP technology which was developed in the Geijsen lab of the Hubrecht Institute.</b> NTrans offered us guidance and assistance with our project by facilitating a lab technician experienced in the work we had to do for our project. We thank them for Clara Martinez’ amazing assistance and patience with our silly questions.</div> |
| | <div><b>Integrated DNA Technologies (IDT) is a company focusing on the development and production of products for life sciences, both for research and diagnostic purposes.</b> They are an important supplier of synthetic DNA & RNA, next generation sequencing services and other tools and products for molecular biology. IDT supports us by offering us 20 kb of free custom DNA.</div> | | <div><b>Integrated DNA Technologies (IDT) is a company focusing on the development and production of products for life sciences, both for research and diagnostic purposes.</b> They are an important supplier of synthetic DNA & RNA, next generation sequencing services and other tools and products for molecular biology. IDT supports us by offering us 20 kb of free custom DNA.</div> |
| | <div><b>MathWorks is the leading company on mathematical computing software.</b> Their two primary products are MATLAB, for the development of algorithms and data-analysis, and Simulink, for designing and running simulations of dynamic, multidomain models. These products are a staple in innovation and development in life sciences as well as other fields. Furthermore, MathWorks’ tools are used in research and educational settings. MathWorks provided us with licenses for their fantastic software and technical support.</div> | | <div><b>MathWorks is the leading company on mathematical computing software.</b> Their two primary products are MATLAB, for the development of algorithms and data-analysis, and Simulink, for designing and running simulations of dynamic, multidomain models. These products are a staple in innovation and development in life sciences as well as other fields. Furthermore, MathWorks’ tools are used in research and educational settings. MathWorks provided us with licenses for their fantastic software and technical support.</div> |
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| | <div style="width: 300px;"><img src="https://static.igem.org/mediawiki/2017/3/38/Uusnapgene.png"></div> | | <div style="width: 300px;"><img src="https://static.igem.org/mediawiki/2017/3/38/Uusnapgene.png"></div> |
| | <div style="width: 275px;"><img src="https://static.igem.org/mediawiki/2017/e/e3/Uuelabjournal.png"></div> | | <div style="width: 275px;"><img src="https://static.igem.org/mediawiki/2017/e/e3/Uuelabjournal.png"></div> |
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| | <div style="width: 250px;"><img src="https://static.igem.org/mediawiki/2017/5/5c/Uuidt.jpg"></div> | | <div style="width: 250px;"><img src="https://static.igem.org/mediawiki/2017/5/5c/Uuidt.jpg"></div> |
| | <div style="width: 300px;"><img src="https://static.igem.org/mediawiki/2017/4/43/Uumathworks.png"></div> | | <div style="width: 300px;"><img src="https://static.igem.org/mediawiki/2017/4/43/Uumathworks.png"></div> |
The OUTCASST two-component system
This year, Utrecht University participates in the iGEM for the first time. We aim to create a cheap DNA detection kit for disease diagnosis that is easy to use and does not rely on complicated sequencing technologies.
The problem
Disease diagnosis is of great importance for healthcare. In developing countries, diagnoses often have to be made based on limited information, even though accurate disease determination based on pathogen specific DNA sequences is possible through sequencing technologies. These technologies, however, require specialised equipment and expertise that simply is not available everywhere. The OUTCASST two-component system and detection kit hopes to alleviate this problem.
The system
The OUTCASST two-component system consists of two proteins, expressed to the membrane of a dryable cell. One of the proteins is a Cas9-fusion and the other contains Cpf1. Both proteins can be given a guide RNA that makes it bind to a specific, user-chosen, complementary sequence. When both proteins bind a DNA fragment from a sample, they co-localize, so that a transcription factor is released intracellularly which then induces an intracellular reporter mechanism such as a dye or fluorescent signal.
Binding of components with search-specific gRNA sequences.
Next
DNA sample fragment binds to one of the components.
Next
Fragment binding with both components induces co-localization.
Next
Protease cleaves, transcription factor is released from complex.
