Difference between revisions of "Team:Amazonas Brazil/Software"

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/* Clear the default wiki settings */
 
/* Clear the default wiki settings */
 
#home_logo, #sideMenu { display:none; } #sideMenu, #top_title, .patrollink {display:none;} #content { width:100%; padding:0px; margin-top:-7px; margin-left:0px;margin-right: 0;} body {background-color:white; } #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 
#home_logo, #sideMenu { display:none; } #sideMenu, #top_title, .patrollink {display:none;} #content { width:100%; padding:0px; margin-top:-7px; margin-left:0px;margin-right: 0;} body {background-color:white; } #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
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<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Amazonas_Brazil/CSS?action=raw&ctype=text/css">
 
<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Amazonas_Brazil/CSS?action=raw&ctype=text/css">
 
<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Amazonas_Brazil/CSS:icon?action=raw&ctype=text/css">
 
<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Amazonas_Brazil/CSS:icon?action=raw&ctype=text/css">
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<title>Wiki_iGEM_Amazonas</title>
 
<title>Wiki_iGEM_Amazonas</title>
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</head>
 
</head>
  
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<body style="width:100%; height:100%; padding: 0; margin: 0;">
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<body class="body">
  
 
   <!-- Add your content of header -->
 
   <!-- Add your content of header -->
  
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<header>
 
     <nav class="navbar navbar-fixed-top navbar-default" style="background-color: rgba(255, 255, 255, 0);">
 
     <nav class="navbar navbar-fixed-top navbar-default" style="background-color: rgba(255, 255, 255, 0);">
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       <ul style="opacity:0.95;list-style-type:none;margin:0;padding:0;background: linear-gradient(to left,#BD314C, #5F254A);overflow:hidden;position: fixed;top: 0;width: 100%;">
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       <ul class="Links_bar" style="background: linear-gradient(to left,#BD314C, #5F254A)">
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         <li><a class="Link-pg" style="padding: 0 1em;" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png" width="104.04" height="90"style="margin-top: 0.3em;"></a></li>
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         <li><a class="Link-pg" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png" width="104.04";height="90"; class="Menu_image"></a></li>
 
       </ul>
 
       </ul>
  
 
       <div class="container">
 
       <div class="container">
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         <button type="button" class="navbar-toggle" style="float: right;">
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         <button type="button" class="navbar-toggle">
 
           <span class="sr-only">Menu</span>
 
           <span class="sr-only">Menu</span>
 
           <span class="icon-bar" style="background-color: white"></span>
 
           <span class="icon-bar" style="background-color: white"></span>
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         <nav class="navbar-fullscreen" id="navbar-middle" style="opacity:0.9;">
 
         <nav class="navbar-fullscreen" id="navbar-middle" style="opacity:0.9;">
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           <button type="button" class="close" aria-label="Close"><span aria-hidden="true" style="top: 0.3em; position: absolute;right: 0.05em;">&times;</span></button>
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           <button type="button" class="close" aria-label="Close"><span aria-hidden="true">&times;</span></button>
  
 
         <div class="main-container">
 
         <div class="main-container">
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           <div class="subcontainer" style="margin-top: 5em;">
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           <div class="subcontainer" id ="top">
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     <a class="Menu" style="margin-right: 0.5em; width: 50%; color: white; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/Project">PROJECT</a>
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     <a class="Menu" id="Link_Menu1" href="https://2017.igem.org/Team:Amazonas_Brazil/Project">PROJECT</a>
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     <a class="Menu" style="width: 50%; color: white; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/Biobricks">BIOBRICKS</a>
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     <a class="Menu" id="Link_Menu2" href="https://2017.igem.org/Team:Amazonas_Brazil/Biobricks">BIOBRICKS</a>
 
     </div>
 
     </div>
 
           <div class="subcontainer">
 
           <div class="subcontainer">
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     <a class="Menu" style=" color: white;margin-right: 0.5em; width: 40%; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/Software">SOFTWARE</a>
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     <a class="Menu" id="Link_Menu3" href="https://2017.igem.org/Team:Amazonas_Brazil/Software">SOFTWARE</a>
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     <a class="Menu" style="width: 60%; color: white; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/Human_Practices">HUMAN PRACTICES</a>
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     <a class="Menu" id="Link_Menu4" href="https://2017.igem.org/Team:Amazonas_Brazil/Human_Practices">HUMAN PRACTICES</a>
 
     </div>
 
     </div>
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     <div class="subcontainer" style="margin-bottom: 1em;">
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     <div class="subcontainer" id="bottom" style="margin-bottom: 1em;">
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<a class="Menu" style="margin-right: 0.5em; width: 70%; color: white; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/Achievements">ACHIEVEMENTS</a>
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<a class="Menu" id="Link_Menu5" href="https://2017.igem.org/Team:Amazonas_Brazil/Achievements">ACHIEVEMENTS</a>
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<a class="Menu" style="width: 30%; color: white; text-decoration: none;" href="https://2017.igem.org/Team:Amazonas_Brazil/People">PEOPLE</a>
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<a class="Menu" id="Link_Menu6" href="https://2017.igem.org/Team:Amazonas_Brazil/People">PEOPLE</a>
 
</div>
 
</div>
 
           </div>
 
           </div>
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   </header>
 
   </header>
  
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   <div class="hero-full-container background-image-container white-text-container" style="background: linear-gradient(to left,#BD314C, #5F254A);">
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   <div class="hero-full-container background-image-container white-text-container" style="background: linear-gradient(to left,#BD314C, #5F254A); width:100%;">
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     <div class="container">
 
     <div class="container">
 
       <div class="row">
 
       <div class="row">
 
         <div class="col-xs-12">
 
         <div class="col-xs-12">
 
           <div class="hero-full-wrapper">
 
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             <div class="text-content  text-center" style="left: 0;margin-left: 0;margin-top: 23%;padding: 0">
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             <div class="text-content  text-center">
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                 <h2 align="middle" style="font-size: 3em; margin: -1em 0 0;padding: 0;">Software</h2>
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                 <h2 class="Page_Title" align="middle">Software</h2>
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                 <h3 align="middle" style="font-size: 1.5em; margin: 0;padding: 0;">A platform to expand the iGEM Experience</h2>
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                 <h2 class="Page_Subtitle" align="middle">A platform to expand the iGEM Experience</h2>
 
             </div>
 
             </div>
 
           </div>
 
           </div>
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         <div class="row section-container-spacer">
 
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<h2>CRISPeasy</h2>
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            <h2>CRISPeasy</h2>
 
             <h3>Our perspective is to provide a bacterial genome editing vector based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR.
 
             <h3>Our perspective is to provide a bacterial genome editing vector based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR.
 
               </h3>
 
               </h3>
  
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               <h4 style="padding: 20px 0 0 0">What's the boost?</h4>
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               <h4 class="p">What's the boost?</h4>
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               <div>
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               <p class="p">Scientific community have been achieved great results using CRISPR-Cas9 mediated
 
               <p class="p">Scientific community have been achieved great results using CRISPR-Cas9 mediated
 
               genome editing techniques. While these methods are moving forward, some methodological
 
               genome editing techniques. While these methods are moving forward, some methodological
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   <p class="footer-share-icons">
 
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     <a href="https://twitter.com/IGEM_Amazonas" class="fa-icon1" title="" style="padding-right: 0; background-color: transparent;">
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     <a href="https://twitter.com/IGEM_Amazonas" class="fa-icon" title="">
 
         <i class="fa fa-twitter" aria-hidden="true"></i>
 
         <i class="fa fa-twitter" aria-hidden="true"></i>
 
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     <a href="https://www.instagram.com/igem_amazonas_brazil/" class="fa-icon1" title="" style="padding-right: 0; background-color: transparent;">
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     <a href="https://www.instagram.com/igem_amazonas_brazil/" class="fa-icon" title="">
 
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         <i class="fa fa-facebook" aria-hidden="true"></i>
 
         <i class="fa fa-facebook" aria-hidden="true"></i>
 
     </a>
 
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Revision as of 03:09, 26 October 2017

Wiki_iGEM_Amazonas

Software

A platform to expand the iGEM Experience

CRISPeasy

Our perspective is to provide a bacterial genome editing vector based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR.

What's the boost?

Scientific community have been achieved great results using CRISPR-Cas9 mediated genome editing techniques. While these methods are moving forward, some methodological aspects related to this technique could be further improved to bring up this science revolution even more.

In prokaryotes, the genome editing based on CRISPR/Cas9 machinery require quite laborious days of work in lab due to the multi-plasmid based edition. The system não é padronizado e, em determinadas metodologias, demands more than one plasmid, each one carrying a part of the CRISPR/Cas9 apparatus. Other critical problem consist in the addition of a linear DNA carrying homologous arms to provide a donor DNA to insert information into the genome during the HDR repair via. However, the linear donor DNA system has low edition efficiency due the action of DNA restriction systems present in wild type and E. coli strains, which can degrade the linear DNAs before integration into genome. All this demands plenty of time and a lot of wet lab hours of work. To verify this information, we got in touch with other iGEM's teams who had work with CRISPR/Cas9 in the previous years, you can check this outreach part with more details here.