Difference between revisions of "Team:Munich/Protocols"

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<input type="checkbox" id="Target" name="type"/> <label for="Target">Target</label>
 
<input type="checkbox" id="Target" name="type"/> <label for="Target">Target</label>
 
<input type="checkbox" id="Other" name="type"/> <label for="Other">Other</label>
 
<input type="checkbox" id="Other" name="type"/> <label for="Other">Other</label>
−
                <div class="myProtocol general">
+
−
<input type="checkbox" id="Phenol-Chloroform-Extraction-RNA">
+
−
<h2><label for="Phenol-Chloroform-Extraction-RNA">Phenol/Chloroform purification of RNA</label></h2>  
+
 
−
<object data="https://static.igem.org/mediawiki/2017/0/0f/T--Munich--Protocols_Phenol_Chloroform_Purification_RNA.pdf" width="100%" height="100%">
+
 
−
<p>Text for when the pdf viewer fails</p>
+
<div class="myProtocol general">
 +
<input type="checkbox" id="agaroseGel">
 +
<h2><label for="agaroseGel">Agarose gel electrophoresis</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--agarose_gel_electrophoresis.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--agarose_gel_electrophoresis.pdf </p>
 
</object>
 
</object>
 
</div>
 
</div>
−
 
+
   
−
                <div class="myProtocol general">
+
<div class="myProtocol general">
−
<input type="checkbox" id="Q5-PCR">
+
<input type="checkbox" id="pcr">
−
<h2><label for="Q5-PCR">PCR amplification with Q5 Master Mix</label></h2>  
+
<h2><label for="pcr">PCR</label></h2>
−
<object data="https://static.igem.org/mediawiki/2017/d/d6/T--Munich--Protocols_Q5_PCR_Amplification.pdf" type="application/pdf" width="100%" height="100%">
+
<object data="https://static.igem.org/mediawiki/2017/0/06/T--Munich--protocol--pcr.pdf" width="100%" height="100%">
−
<p>Text for when the pdf viewer fails</p>
+
<p> https://static.igem.org/mediawiki/2017/0/06/T--Munich--protocol--pcr.pdf </p>
 
</object>
 
</object>
 
</div>
 
</div>
−
                <div class="myProtocol general">
+
   
−
<input type="checkbox" id="RT-PCR-MuLV">
+
    <div class="myProtocol general">
−
<h2><label for="RT-PCR-MuLV">First strand cDNA synthesis</label></h2>  
+
<input type="checkbox" id="miniprep ">
−
<object data="https://static.igem.org/mediawiki/2017/7/72/T--Munich--Protocols_RT-PCR_MuLV_Kit.pdf" width="100%" height="100%">
+
<h2><label for="miniprep ">Miniprep </label></h2>
−
<p>Text for when the pdf viewer fails</p>
+
<object data="https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--miniprep.pdf" width="100%" height="100%">
 +
<p>https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--miniprep.pdf </p>
 
</object>
 
</object>
 
</div>
 
</div>
−
 
+
   
−
                <div class="myProtocol cas13a">
+
   
−
<input type="checkbox" id="Ni-NTAPurification">
+
   
−
<h2><label for="Ni-NTAPurification">Ni-NTA-Agarose Purification of His-Tag-Cas13a</label></h2>  
+
<div class="myProtocol general">
−
<object data="https://static.igem.org/mediawiki/2017/3/39/T--Munich--Protocols_ProteinPurficationWithNiNTA.pdf" width="100%" height="100%">
+
<input type="checkbox" id="restrictiondigest">
−
<p>Text for when the pdf viewer fails</p>
+
<h2><label for="restrictionddigest">Restriction digest</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/8/83/T--Munich--protocol--restriction_digest.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/8/83/T--Munich--protocol--restriction_digest.pdf </p>
 
</object>
 
</object>
 
</div>
 
</div>
−
<div class="myProtocol other">
+
   
−
<input type="checkbox" id="TotalRNAPurificationNorgen">
+
    <div class="myProtocol general">
−
<h2 id="lolxd"><label for="TotalRNAPurificationNorgen">Total RNA Purification with Norgen Kit</label></h2>  
+
<input type="checkbox" id="Ligation">
−
<object data="https://static.igem.org/mediawiki/2017/a/af/T--Munich--Protocols_Norgen_Total_RNA_Purification.pdf" type="application/pdf" width="100%" height="100%">
+
<h2><label for="Ligation">Ligation</label></h2>
−
<p>Text for when the pdf viewer fails</p>
+
<object data="https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--ligation.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--ligation.pdf </p>
 
</object>
 
</object>
 
</div>
 
</div>
−
<div class="myProtocol other">
+
   
−
<input type="checkbox" id="FINAEXtractionDNA">
+
        <div class="myProtocol general">
−
<h2><label for="FINAEXtractionDNA">FINA Extraction for DNA</label></h2>
+
<input type="checkbox" id="Glycerolstock">
−
<object data="https://static.igem.org/mediawiki/2017/4/41/T--Munich--Protocols_FINA_Extraction_DNA.pdf" type="application/pdf" width="100%" height="100%">
+
<h2><label for="Glycerolstock">Glycerol stock</label></h2>
−
<p>Text for when the pdf viewer fails</p>
+
<object data="https://static.igem.org/mediawiki/2017/f/fc/T--Munich--protocol--glycerol_stock.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/f/fc/T--Munich--protocol--glycerol_stock.pdf </p>
 
</object>
 
</object>
 
</div>
 
</div>
−
<div class="myProtocol other">
+
   
−
<input type="checkbox" id="FINAEXtractionRNA">
+
   
−
<h2><label for="FINAEXtractionRNA">FINA Extraction for RNA</label></h2>
+
  <div class="myProtocol cas13a">
−
<object data="https://static.igem.org/mediawiki/2017/5/58/T--Munich--Protocols_FINA_Extraction_RNA.pdf" width="100%" height="100%">
+
<input type="checkbox" id="protpurNiNTA">
−
<p>Text for when the pdf viewer fails</p>
+
<h2><label for="protpurNiNTA">Protein purification with Ni-NTA</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/b/bc/T--Munich--protocol--protein_purification_Ni_NTA.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/b/bc/T--Munich--protocol--protein_purification_Ni_NTA.pdf </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol cas13a">
 +
<input type="checkbox" id="protpurAkta">
 +
<h2><label for="protpurAkta">Protein purification with Akta</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--cas13a_purif_akta.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--cas13a_purif_akta.pdf </p>
 +
</object>
 +
</div>
 +
 
 +
  <div class="myProtocol general">
 +
<input type="checkbox" id="SDSpage">
 +
<h2><label for="SDSpage">SDS page </label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/2/21/T--Munich--protocol--sds_page.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/2/21/T--Munich--protocol--sds_page.pdf </p>
 +
</object>
 +
</div>
 +
 
 +
   
 +
   
 +
  <div class="myProtocol other">
 +
<input type="checkbox" id="RNAseActi">
 +
<h2><label for="RNAseActi">RNAse activity protocol</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/4/40/T--Munich--protocol--rnase_activity.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/4/40/T--Munich--protocol--rnase_activity.pdf </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol cas13a">
 +
<input type="checkbox" id="lyophilization">
 +
<h2><label for="lyophilization">Lyophilization</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/f/f4/T--Munich--protocol--lyophilization.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/f/f4/T--Munich--protocol--lyophilization.pdf </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol general">
 +
<input type="checkbox" id="expression">
 +
<h2><label for="expression">Protein expression</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/1/19/T--Munich--protocol--protein_expression.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/1/19/T--Munich--protocol--protein_expression.pdf </p>
 +
</object>
 +
</div>
 +
 +
 +
  <div class="myProtocol other">
 +
<input type="checkbox" id="pheChlorRNA">
 +
<h2><label for="pheChlorRNA">phenol-chlorophorm purification of RNA</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/0/0b/T--Munich--protocol--phenol_chlorophorm_purification_rna.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/0/0b/T--Munich--protocol--phenol_chlorophorm_purification_rna.pdf </p>
 +
</object>
 +
</div>
 +
 +
<div class="myProtocol other">
 +
<input type="checkbox" id="PheChloroDNA">
 +
<h2><label for="PheChloroDNA">Phenol-chlorophorm purification of DNA</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/2/2b/T--Munich--protocol--phenol_chlorophorm_purification_dna.pdf" width="100%" height="100%">
 +
<p>https://static.igem.org/mediawiki/2017/2/2b/T--Munich--protocol--phenol_chlorophorm_purification_dna.pdf</p>
 
</object>
 
</object>
 
</div>
 
</div>
 
     </div>
 
     </div>
 +
   
 +
  <div class="myProtocol other">
 +
<input type="checkbox" id="UreaRNA">
 +
<h2><label for="UreaRNA">Urea gel for RNA detection </label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/c/cc/T--Munich--protocol--urea_page.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/c/cc/T--Munich--protocol--urea_page.pdf </p>
 +
</object>
 +
</div>
 +
 +
 +
  <div class="myProtocol other">
 +
<input type="checkbox" id="norgen">
 +
<h2><label for="norgen">RNA purification with Norgen Kit</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/3/3f/T--Munich--protocol--rna_purification_norgenkit.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/3/3f/T--Munich--protocol--rna_purification_norgenkit.pdf </p>
 +
</object>
 +
</div>
 +
 +
 +
  <div class="myProtocol other">
 +
<input type="checkbox" id="finaRNA">
 +
<h2><label for="finaRNA">FINA method for RNA extraction</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--fina_extraction_rna.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--fina_extraction_rna.pdf </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol other">
 +
<input type="checkbox" id="finaDNA">
 +
<h2><label for="finaDNA">FINA method for DNA extraction</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--fina_extraction_dna.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--fina_extraction_dna.pdf </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol general">
 +
<input type="checkbox" id="cDNA">
 +
<h2><label for="cDNA">First strand cDNA syntesis (NEB)</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--first_strand_cdna_synthesis.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--first_strand_cdna_synthesis.pdf </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol other">
 +
<input type="checkbox" id="RPA">
 +
<h2><label for="RPA">RPA</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/f/f7/T--Munich--protocol--rpa.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/f/f7/T--Munich--protocol--rpa.pdf </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol other">
 +
<input type="checkbox" id="RPApaper">
 +
<h2><label for="RPApaper">RPA on paper</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/1/15/T--Munich--protocol--rpa_paper.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/1/15/T--Munich--protocol--rpa_paper.pdf </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol other">
 +
<input type="checkbox" id="RPATX">
 +
<h2><label for="RPATX">RPA TX</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/d/df/T--Munich--protocol--rpa_tx.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/d/df/T--Munich--protocol--rpa_tx.pdf </p>
 +
</object>
 +
</div>
 +
 +
 +
 +
  <div class="myProtocol general">
 +
<input type="checkbox" id="Chemtrafo">
 +
<h2><label for="Chemtrafo">Chem trafo</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/5/5d/T--Munich--protocol--chem_trafo.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/5/5d/T--Munich--protocol--chem_trafo.pdf </p>
 +
</object>
 +
</div>
 +
 +
 +
  <div class="myProtocol general">
 +
<input type="checkbox" id="Electrotrafo">
 +
<h2><label for="Electrotrafo">Electro trafo</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/7/7e/T--Munich--protocol--e_trafo.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/7/7e/T--Munich--protocol--e_trafo.pdf </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol general">
 +
<input type="checkbox" id="Chemcells">
 +
<h2><label for="Chemcells">Chemically competent cells</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/8/81/T--Munich--protocol--chem_comp_cells.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/8/81/T--Munich--protocol--chem_comp_cells.pdf </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol general">
 +
<input type="checkbox" id="ecells">
 +
<h2><label for="ecells">Electro-competent cells</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--e_comp_cells.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--e_comp_cells.pdf </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol readout">
 +
<input type="checkbox" id="Dephosphorylation">
 +
<h2><label for="Dephosphorylation">Dephosphorylation</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/3/3e/T--Munich--protocol--dephosphorylation.pdf" width="100%" height="100%">
 +
<p>  </p>
 +
</object>
 +
</div>
 +
 +
  <div class="myProtocol readout">
 +
<input type="checkbox" id="GoldenGate">
 +
<h2><label for="GoldenGate">Golden Gate</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/9/9c/T--Munich--protocol--golden_gate.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/9/9c/T--Munich--protocol--golden_gate.pdf </p>
 +
</object>
 +
</div>
 +
 +
 +
  <div class="myProtocol readout">
 +
<input type="checkbox" id="Gibson">
 +
<h2><label for="Gibson ">Gibson</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/f/fa/T--Munich--protocol--gibson.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/f/fa/T--Munich--protocol--gibson.pdf </p>
 +
</object>
 +
</div>
 +
  <div class="myProtocol general">
 +
<input type="checkbox" id="PCRcleanup">
 +
<h2><label for="PCRcleanup">PCR cleanup</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/0/05/T--Munich--protocol--pcr_clean_up.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/0/05/T--Munich--protocol--pcr_clean_up.pdf </p>
 +
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 +
</div>
 +
  <div class="myProtocol other">
 +
<input type="checkbox" id="Softlitography">
 +
<h2><label for="Softlitography">Soft litography</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/1/16/T--Munich--protocol--soft_lithography.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/1/16/T--Munich--protocol--soft_lithography.pdf </p>
 +
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 +
</div>
 +
  <div class="myProtocol readout">
 +
<input type="checkbox" id="AuNPpreparation">
 +
<h2><label for="AuNPpreparation">AuNP preparation</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/2/22/T--Munich--protocol--au_np_preparation.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/2/22/T--Munich--protocol--au_np_preparation.pdf </p>
 +
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 +
</div>
 +
 +
 +
  <div class="myProtocol readout">
 +
<input type="checkbox" id="AuNPDNAconjugation">
 +
<h2><label for="AuNPDNAconjugation">AuNP-DNA conjugation</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/1/17/T--Munich--protocol--au_np_dna_conjugation.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/1/17/T--Munich--protocol--au_np_dna_conjugation.pdf </p>
 +
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 +
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 +
 +
 +
  <div class="myProtocol readout">
 +
<input type="checkbox" id="AuNPlinkage">
 +
<h2><label for="AuNPlinkage">AuNP linkage</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/1/1d/T--Munich--protocol--au_np_linkage.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/1/1d/T--Munich--protocol--au_np_linkage.pdf </p>
 +
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 +
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 +
 +
 +
  <div class="myProtocol readout">
 +
<input type="checkbox" id="AuNPcleavage">
 +
<h2><label for="AuNPcleavage">AuNP cleavage</label></h2>
 +
<object data="https://static.igem.org/mediawiki/2017/e/ee/T--Munich--protocol--au_np_cleavage.pdf" width="100%" height="100%">
 +
<p> https://static.igem.org/mediawiki/2017/e/ee/T--Munich--protocol--au_np_cleavage.pdf </p>
 +
</object>
 +
</div>
  
  

Revision as of 12:40, 26 October 2017

Description

Design

Demonstrate

Measurement

Applied Design

Final Results

Achievements

Protocols

Notebook

Safety

Parts

Improved Part

Part Collection

InterLab

Model

Software

Collaboration

Detector

Quake Valve

Paperstrips

Sample Processing

Silver

Gold

Engagement

Collaborations

Team members

Sponsors

Attributions

Gallery

Protocols

According to all known laws of aviation, there is no way a bee should be able to fly. Its wings are too small to get its fat little body off the ground. The bee, of course, flies anyway because bees don't care what humans think is impossible. Yellow, black. Yellow, black. Yellow, black. Yellow, black.

https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--agarose_gel_electrophoresis.pdf

https://static.igem.org/mediawiki/2017/0/06/T--Munich--protocol--pcr.pdf

https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--miniprep.pdf

https://static.igem.org/mediawiki/2017/8/83/T--Munich--protocol--restriction_digest.pdf

https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--ligation.pdf

https://static.igem.org/mediawiki/2017/f/fc/T--Munich--protocol--glycerol_stock.pdf

https://static.igem.org/mediawiki/2017/b/bc/T--Munich--protocol--protein_purification_Ni_NTA.pdf

https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--cas13a_purif_akta.pdf

https://static.igem.org/mediawiki/2017/2/21/T--Munich--protocol--sds_page.pdf

https://static.igem.org/mediawiki/2017/4/40/T--Munich--protocol--rnase_activity.pdf

https://static.igem.org/mediawiki/2017/f/f4/T--Munich--protocol--lyophilization.pdf

https://static.igem.org/mediawiki/2017/1/19/T--Munich--protocol--protein_expression.pdf

https://static.igem.org/mediawiki/2017/0/0b/T--Munich--protocol--phenol_chlorophorm_purification_rna.pdf

https://static.igem.org/mediawiki/2017/2/2b/T--Munich--protocol--phenol_chlorophorm_purification_dna.pdf

https://static.igem.org/mediawiki/2017/c/cc/T--Munich--protocol--urea_page.pdf

https://static.igem.org/mediawiki/2017/3/3f/T--Munich--protocol--rna_purification_norgenkit.pdf

https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--fina_extraction_rna.pdf

https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--fina_extraction_dna.pdf

https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--first_strand_cdna_synthesis.pdf

https://static.igem.org/mediawiki/2017/f/f7/T--Munich--protocol--rpa.pdf

https://static.igem.org/mediawiki/2017/1/15/T--Munich--protocol--rpa_paper.pdf

https://static.igem.org/mediawiki/2017/d/df/T--Munich--protocol--rpa_tx.pdf

https://static.igem.org/mediawiki/2017/5/5d/T--Munich--protocol--chem_trafo.pdf

https://static.igem.org/mediawiki/2017/7/7e/T--Munich--protocol--e_trafo.pdf

https://static.igem.org/mediawiki/2017/8/81/T--Munich--protocol--chem_comp_cells.pdf

https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--e_comp_cells.pdf

https://static.igem.org/mediawiki/2017/9/9c/T--Munich--protocol--golden_gate.pdf

https://static.igem.org/mediawiki/2017/f/fa/T--Munich--protocol--gibson.pdf

https://static.igem.org/mediawiki/2017/0/05/T--Munich--protocol--pcr_clean_up.pdf

https://static.igem.org/mediawiki/2017/1/16/T--Munich--protocol--soft_lithography.pdf

https://static.igem.org/mediawiki/2017/2/22/T--Munich--protocol--au_np_preparation.pdf

https://static.igem.org/mediawiki/2017/1/17/T--Munich--protocol--au_np_dna_conjugation.pdf

https://static.igem.org/mediawiki/2017/1/1d/T--Munich--protocol--au_np_linkage.pdf

https://static.igem.org/mediawiki/2017/e/ee/T--Munich--protocol--au_np_cleavage.pdf

Primers and sequences