Difference between revisions of "Team:Munich/Protocols"

Revision as of 13:02, 26 October 2017

Protocols
According to all known laws of aviation, there is no way a bee should be able to fly. Its wings are too small to get its fat little body off the ground. The bee, of course, flies anyway because bees don't care what humans think is impossible. Yellow, black. Yellow, black. Yellow, black. Yellow, black.
General Cas13a Read-out Target Other Agarose gel electrophoresis PCR Miniprep Restriction digest Ligation Glycerol stock Protein purification with Ni-NTA Protein purification with Akta SDS page RNAse activity protocol Lyophilization Protein expression phenol-chlorophorm purification of RNA Phenol-chlorophorm purification of DNA Urea gel for RNA detection RNA purification with Norgen Kit FINA method for RNA extraction FINA method for DNA extraction First strand cDNA syntesis (NEB) RPA RPA on paper RPA TX Chem trafo Electro trafo Chemically competent cells Electro-competent cells Dephosphorylation Golden Gate Gibson PCR cleanup Soft litography AuNP preparation AuNP-DNA conjugation AuNP linkage AuNP cleavage
Primers and sequences

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