Safety

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Safe lab work

Concerning the safety in the lab, we all had a lecture by Chantal SOSCIA, the prevention officer, about the risks and precautions. This lecture was prior to the wet lab beginning. She told us about basic lab rules (for instance, washing hands before and after an experiment) and basic precautions for our safety: wearing a cotton lab coat, gloves, glasses (if needed), long pants, closed shoes, to have long hair tied. Then she told us about biological and chemical risks, to be aware of the risks before starting an experiment to take the right precautions, how to store the different products. She taught us the risks of several possible cases (like liquid nitrogen, sonicator, etc.) and what precautions to take in each case. And also, the disposal procedures of chemical and biological wastes. And at the end, she gave us a booklet summarizing everything she told us. In case of emergency, we had a lifeguard in the lab next door. And he showed us where the extinguishers were. We were particularly careful when using toxic chemical compound: - GelRed for agarose gel revelation: we were wearing a glove on the hand touching the contaminated things (spatula, gel, revealing box). - Chloroform: handled under the fuming hood. The experiments on Pseudomonas aeruginosa where carried out by Gauthier DANGLA-PELISSIER, a PhD student, in a level 2 lab, respecting the rules fit for this kind of lab.

Safe project design

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Safe shipment

Concerning the shipment of the parts for the Registry, there was no biological issues since the DNA is dry and there are no bacteria. We just followed the protocol and packed everything in a box.