Rstclair2012 (Talk | contribs) |
Rstclair2012 (Talk | contribs) |
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<center><h4>Member Participations</h4></center> | <center><h4>Member Participations</h4></center> | ||
<h5>Calibrations:</h5> | <h5>Calibrations:</h5> | ||
− | <p>- Completed by Douglas + Valentina on September 21st</p> | + | <p> - Completed by Douglas + Valentina on September 21st</p> |
<br/> | <br/> | ||
<h5>Transformations:</h5> | <h5>Transformations:</h5> | ||
− | <p>- Completed by Douglas + Ariania and supervised by Dr. Pavlovic on September 26th</p> | + | <p> - Completed by Douglas + Ariania and supervised by Dr. Pavlovic on September 26th</p> |
<br/> | <br/> | ||
<h5>Cell Measurement:</h5> | <h5>Cell Measurement:</h5> | ||
− | <p>- Completed by Douglas + Rachel S. and assisted by Eric and Daniela (Esiobu lab members) on September 27th, 28th, 29th</p> | + | <p> - Completed by Douglas + Rachel S. and assisted by Eric and Daniela (Esiobu lab members) on September 27th, 28th, 29th</p> |
<br/> | <br/> | ||
<br/> | <br/> | ||
<center><h4>Methodology</h4></center> | <center><h4>Methodology</h4></center> | ||
<h5>Materials</h5> | <h5>Materials</h5> | ||
− | + | <p> ● Competent cells (Escherichia coli strain DH5α) | |
<br/> | <br/> | ||
● LB (Luria Bertani) media | ● LB (Luria Bertani) media | ||
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◻ Recorded the data in the table below or in notebook | ◻ Recorded the data in the table below or in notebook | ||
<br/> | <br/> | ||
− | ◻ Imported data into Excel (OD600 reference point tab) Sheet_1 provided | + | ◻ Imported data into Excel (OD600 reference point tab) Sheet_1 provided </p> |
<br/> | <br/> | ||
<h5>Transformation Procedure</h5> | <h5>Transformation Procedure</h5> | ||
− | ◻ added 5uL DNA | + | <p> ◻ added 5uL DNA |
<br/> | <br/> | ||
◻ let sit 30 min on ice | ◻ let sit 30 min on ice | ||
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<h5>Cell Measurement Procedure</h5> | <h5>Cell Measurement Procedure</h5> | ||
<h6>Day 1:</h6> | <h6>Day 1:</h6> | ||
− | transform Escherichia coli DH5α with these following plasmids: | + | <p>transform Escherichia coli DH5α with these following plasmids:</p> |
<br/> | <br/> | ||
<p>● Positive control | <p>● Positive control |
Revision as of 19:57, 26 October 2017
Florida_Atlantic