Difference between revisions of "Team:Munich/Protocols"
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<h2><label for="agaroseGel">Agarose gel electrophoresis</label></h2> | <h2><label for="agaroseGel">Agarose gel electrophoresis</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--agarose_gel_electrophoresis.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--agarose_gel_electrophoresis.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--agarose_gel_electrophoresis.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--agarose_gel_electrophoresis.pdf" > Click here! </a> </p> |
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<h2><label for="pcr">PCR</label></h2> | <h2><label for="pcr">PCR</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/0/06/T--Munich--protocol--pcr.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/0/06/T--Munich--protocol--pcr.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/0/06/T--Munich--protocol--pcr.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/0/06/T--Munich--protocol--pcr.pdf" > Click here! </a> </p> |
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<h2><label for="miniprep ">Miniprep </label></h2> | <h2><label for="miniprep ">Miniprep </label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--miniprep.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--miniprep.pdf" width="100%" height="100%"> | ||
| − | <p>https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--miniprep.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--miniprep.pdf" > Click here! </a> </p> |
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<h2><label for="restrictionddigest">Restriction digest</label></h2> | <h2><label for="restrictionddigest">Restriction digest</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/8/83/T--Munich--protocol--restriction_digest.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/8/83/T--Munich--protocol--restriction_digest.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/8/83/T--Munich--protocol--restriction_digest.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/8/83/T--Munich--protocol--restriction_digest.pdf" > Click here! </a> </p> |
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<h2><label for="Ligation">Ligation</label></h2> | <h2><label for="Ligation">Ligation</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--ligation.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--ligation.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--ligation.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--ligation.pdf" > Click here! </a> </p> |
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<h2><label for="Glycerolstock">Glycerol stock</label></h2> | <h2><label for="Glycerolstock">Glycerol stock</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/f/fc/T--Munich--protocol--glycerol_stock.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/f/fc/T--Munich--protocol--glycerol_stock.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/f/fc/T--Munich--protocol--glycerol_stock.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/f/fc/T--Munich--protocol--glycerol_stock.pdf" > Click here! </a> </p> |
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<h2><label for="protpurNiNTA">Protein purification with Ni-NTA</label></h2> | <h2><label for="protpurNiNTA">Protein purification with Ni-NTA</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/b/bc/T--Munich--protocol--protein_purification_Ni_NTA.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/b/bc/T--Munich--protocol--protein_purification_Ni_NTA.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/b/bc/T--Munich--protocol--protein_purification_Ni_NTA.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/b/bc/T--Munich--protocol--protein_purification_Ni_NTA.pdf" > Click here! </a> </p> |
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<h2><label for="protpurAkta">Protein purification with Akta</label></h2> | <h2><label for="protpurAkta">Protein purification with Akta</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--cas13a_purif_akta.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--cas13a_purif_akta.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--cas13a_purif_akta.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--cas13a_purif_akta.pdf" > Click here! </a> </p> |
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<h2><label for="SDSpage">SDS page </label></h2> | <h2><label for="SDSpage">SDS page </label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/2/21/T--Munich--protocol--sds_page.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/2/21/T--Munich--protocol--sds_page.pdf" width="100%" height="100%"> | ||
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<h2><label for="RNAseActi">RNAse activity protocol</label></h2> | <h2><label for="RNAseActi">RNAse activity protocol</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/4/40/T--Munich--protocol--rnase_activity.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/4/40/T--Munich--protocol--rnase_activity.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/4/40/T--Munich--protocol--rnase_activity.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/4/40/T--Munich--protocol--rnase_activity.pdf" > Click here! </a> </p> |
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<h2><label for="lyophilization">Lyophilization</label></h2> | <h2><label for="lyophilization">Lyophilization</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/f/f4/T--Munich--protocol--lyophilization.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/f/f4/T--Munich--protocol--lyophilization.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/f/f4/T--Munich--protocol--lyophilization.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/f/f4/T--Munich--protocol--lyophilization.pdf" > Click here! </a> </p> |
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<h2><label for="expression">Protein expression</label></h2> | <h2><label for="expression">Protein expression</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/1/19/T--Munich--protocol--protein_expression.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/1/19/T--Munich--protocol--protein_expression.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/1/19/T--Munich--protocol--protein_expression.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/1/19/T--Munich--protocol--protein_expression.pdf" > Click here! </a> </p> |
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<h2><label for="pheChlorRNA">phenol-chlorophorm purification of RNA</label></h2> | <h2><label for="pheChlorRNA">phenol-chlorophorm purification of RNA</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/0/0b/T--Munich--protocol--phenol_chlorophorm_purification_rna.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/0/0b/T--Munich--protocol--phenol_chlorophorm_purification_rna.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/0/0b/T--Munich--protocol--phenol_chlorophorm_purification_rna.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/0/0b/T--Munich--protocol--phenol_chlorophorm_purification_rna.pdf" > Click here! </a> </p> |
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<h2><label for="PheChloroDNA">Phenol-chlorophorm purification of DNA</label></h2> | <h2><label for="PheChloroDNA">Phenol-chlorophorm purification of DNA</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/2/2b/T--Munich--protocol--phenol_chlorophorm_purification_dna.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/2/2b/T--Munich--protocol--phenol_chlorophorm_purification_dna.pdf" width="100%" height="100%"> | ||
| − | <p>https://static.igem.org/mediawiki/2017/2/2b/T--Munich--protocol--phenol_chlorophorm_purification_dna.pdf</p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/2/2b/T--Munich--protocol--phenol_chlorophorm_purification_dna.pdf" > Click here! </a> </p> |
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<h2><label for="UreaRNA">Urea gel for RNA detection </label></h2> | <h2><label for="UreaRNA">Urea gel for RNA detection </label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/c/cc/T--Munich--protocol--urea_page.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/c/cc/T--Munich--protocol--urea_page.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/c/cc/T--Munich--protocol--urea_page.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/c/cc/T--Munich--protocol--urea_page.pdf" > Click here! </a> </p> |
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<h2><label for="norgen">RNA purification with Norgen Kit</label></h2> | <h2><label for="norgen">RNA purification with Norgen Kit</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/3/3f/T--Munich--protocol--rna_purification_norgenkit.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/3/3f/T--Munich--protocol--rna_purification_norgenkit.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/3/3f/T--Munich--protocol--rna_purification_norgenkit.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/3/3f/T--Munich--protocol--rna_purification_norgenkit.pdf" > Click here! </a> </p> |
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<h2><label for="finaRNA">FINA method for RNA extraction</label></h2> | <h2><label for="finaRNA">FINA method for RNA extraction</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--fina_extraction_rna.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--fina_extraction_rna.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--fina_extraction_rna.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--fina_extraction_rna.pdf" > Click here! </a> </p> |
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<h2><label for="finaDNA">FINA method for DNA extraction</label></h2> | <h2><label for="finaDNA">FINA method for DNA extraction</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--fina_extraction_dna.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--fina_extraction_dna.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--fina_extraction_dna.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--fina_extraction_dna.pdf" > Click here! </a> </p> |
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<h2><label for="cDNA">First strand cDNA syntesis (NEB)</label></h2> | <h2><label for="cDNA">First strand cDNA syntesis (NEB)</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--first_strand_cdna_synthesis.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--first_strand_cdna_synthesis.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--first_strand_cdna_synthesis.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--first_strand_cdna_synthesis.pdf" > Click here! </a> </p> |
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<h2><label for="RPA">RPA</label></h2> | <h2><label for="RPA">RPA</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/f/f7/T--Munich--protocol--rpa.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/f/f7/T--Munich--protocol--rpa.pdf" width="100%" height="100%"> | ||
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<h2><label for="RPApaper">RPA on paper</label></h2> | <h2><label for="RPApaper">RPA on paper</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/1/15/T--Munich--protocol--rpa_paper.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/1/15/T--Munich--protocol--rpa_paper.pdf" width="100%" height="100%"> | ||
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<h2><label for="RPATX">RPA TX</label></h2> | <h2><label for="RPATX">RPA TX</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/d/df/T--Munich--protocol--rpa_tx.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/d/df/T--Munich--protocol--rpa_tx.pdf" width="100%" height="100%"> | ||
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<h2><label for="Chemtrafo">Chem trafo</label></h2> | <h2><label for="Chemtrafo">Chem trafo</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/5/5d/T--Munich--protocol--chem_trafo.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/5/5d/T--Munich--protocol--chem_trafo.pdf" width="100%" height="100%"> | ||
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<h2><label for="Electrotrafo">Electro trafo</label></h2> | <h2><label for="Electrotrafo">Electro trafo</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/7/7e/T--Munich--protocol--e_trafo.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/7/7e/T--Munich--protocol--e_trafo.pdf" width="100%" height="100%"> | ||
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<h2><label for="Chemcells">Chemically competent cells</label></h2> | <h2><label for="Chemcells">Chemically competent cells</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/8/81/T--Munich--protocol--chem_comp_cells.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/8/81/T--Munich--protocol--chem_comp_cells.pdf" width="100%" height="100%"> | ||
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<h2><label for="ecells">Electro-competent cells</label></h2> | <h2><label for="ecells">Electro-competent cells</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--e_comp_cells.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--e_comp_cells.pdf" width="100%" height="100%"> | ||
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<h2><label for="Dephosphorylation">Dephosphorylation</label></h2> | <h2><label for="Dephosphorylation">Dephosphorylation</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/3/3e/T--Munich--protocol--dephosphorylation.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/3/3e/T--Munich--protocol--dephosphorylation.pdf" width="100%" height="100%"> | ||
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<h2><label for="GoldenGate">Golden Gate</label></h2> | <h2><label for="GoldenGate">Golden Gate</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/9/9c/T--Munich--protocol--golden_gate.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/9/9c/T--Munich--protocol--golden_gate.pdf" width="100%" height="100%"> | ||
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<h2><label for="Gibson ">Gibson</label></h2> | <h2><label for="Gibson ">Gibson</label></h2> | ||
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<h2><label for="PCRcleanup">PCR cleanup</label></h2> | <h2><label for="PCRcleanup">PCR cleanup</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/0/05/T--Munich--protocol--pcr_clean_up.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/0/05/T--Munich--protocol--pcr_clean_up.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/0/05/T--Munich--protocol--pcr_clean_up.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/0/05/T--Munich--protocol--pcr_clean_up.pdf" > Click here! </a> </p> |
| + | </object> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="myProtocol general"> | ||
| + | <input type="checkbox" id="seqprep"> | ||
| + | <h2><label for="seqprep">Sequencing sample prep</label></h2> | ||
| + | <object data="https://static.igem.org/mediawiki/2017/5/5b/T--Munich--protocol--sequencing_prep.pdf" width="100%" height="100%"> | ||
| + | <p> <a src="https://static.igem.org/mediawiki/2017/5/5b/T--Munich--protocol--sequencing_prep.pdf" > Click here! </a> </p> | ||
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<h2><label for="Softlitography">Soft litography</label></h2> | <h2><label for="Softlitography">Soft litography</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/1/16/T--Munich--protocol--soft_lithography.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/1/16/T--Munich--protocol--soft_lithography.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/1/16/T--Munich--protocol--soft_lithography.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/1/16/T--Munich--protocol--soft_lithography.pdf" > Click here! </a> </p> |
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<h2><label for="AuNPpreparation">AuNP preparation</label></h2> | <h2><label for="AuNPpreparation">AuNP preparation</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/2/22/T--Munich--protocol--au_np_preparation.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/2/22/T--Munich--protocol--au_np_preparation.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/2/22/T--Munich--protocol--au_np_preparation.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/2/22/T--Munich--protocol--au_np_preparation.pdf" > Click here! </a> </p> |
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<h2><label for="AuNPDNAconjugation">AuNP-DNA conjugation</label></h2> | <h2><label for="AuNPDNAconjugation">AuNP-DNA conjugation</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/1/17/T--Munich--protocol--au_np_dna_conjugation.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/1/17/T--Munich--protocol--au_np_dna_conjugation.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/1/17/T--Munich--protocol--au_np_dna_conjugation.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/1/17/T--Munich--protocol--au_np_dna_conjugation.pdf" > Click here! </a> </p> |
</object> | </object> | ||
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<h2><label for="AuNPlinkage">AuNP linkage</label></h2> | <h2><label for="AuNPlinkage">AuNP linkage</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/1/1d/T--Munich--protocol--au_np_linkage.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/1/1d/T--Munich--protocol--au_np_linkage.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/1/1d/T--Munich--protocol--au_np_linkage.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/1/1d/T--Munich--protocol--au_np_linkage.pdf" > Click here! </a> </p> |
</object> | </object> | ||
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<h2><label for="AuNPcleavage">AuNP cleavage</label></h2> | <h2><label for="AuNPcleavage">AuNP cleavage</label></h2> | ||
<object data="https://static.igem.org/mediawiki/2017/e/ee/T--Munich--protocol--au_np_cleavage.pdf" width="100%" height="100%"> | <object data="https://static.igem.org/mediawiki/2017/e/ee/T--Munich--protocol--au_np_cleavage.pdf" width="100%" height="100%"> | ||
| − | <p> https://static.igem.org/mediawiki/2017/e/ee/T--Munich--protocol--au_np_cleavage.pdf </p> | + | <p> <a src="https://static.igem.org/mediawiki/2017/e/ee/T--Munich--protocol--au_np_cleavage.pdf" > Click here! </a> </p> |
</object> | </object> | ||
</div> | </div> | ||
Revision as of 20:23, 26 October 2017
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