Difference between revisions of "Team:Lethbridge HS/Contribution"

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<br> <br> <br>   
 
<br> <br> <br>   
 
<div class="container-fluid" style="margin-left:10%; margin-right:10%;">
 
<div class="container-fluid" style="margin-left:10%; margin-right:10%;">
−
<h2><b>&nbsp;Basic Parts</b></h2>
+
<h1><b>&nbsp;Basic Parts</b></h1>
 
<br>
 
<br>
 +
<h3><i><b>indB</i>B</b></h3>
 
<p class="center">
 
<p class="center">
−
All of our parts were submitted in the iGEM plasmid psB1C3.
+
<a href="">BBa_K1281001</a>
 +
<br>
 +
This Gene is from the organism <i>Petunia hybrid</i>. It has been shwo to increase the yeilds of Indigoidine when paired with the gene <i>ind</i>C. We could not separate this gene from its composite part so we have submitted the nucleotide sequence to the registry.
 
</p>
 
</p>
 +
<br>
 +
<br>
  
 
<h3><i><b>3gt</b></i></h3>
 
<h3><i><b>3gt</b></i></h3>
−
<p class="center"> The gene <i>3gt</i> is from the anthocyanin synthesis pathway and converts the initial molecule Pelargonidin into Anthocyanin. This gene is from the organism <i>Petunia hybrid</i>. We have added this part to the registry as one of our new basic part submissions. Part BBa_idontknowwewillfindout
+
<p class="center">  
−
</p>
+
<a href="">BBa_K281003</a>
 
<br>
 
<br>
 +
The gene <i>3gt</i> is from the anthocyanin synthesis pathway and converts the initial molecule Pelargonidin into Anthocyanin. This gene is from the organism <i>Petunia hybrid</i>. We have added this part to the registry as one of our new basic part submissions. We could not get this gene out of its composite part so we added the sequence to the registry.
 +
<br>
 +
<br>
 +
 
<h3><b><i>yad</i>H</b></h3>
 
<h3><b><i>yad</i>H</b></h3>
−
<p class="center">This Gene is an <i>Escherichia coli</i> gene that has been shown to increase the yields of anthocyanin when paired with the genes in our anthocyanin construct. It is our second original basic part submission to the registry. It is uncharacterized and is a nter-membrane transport protein. Part BBa_wowzers.
+
<p class="center">
 +
<a href="">BBa_K1281002</a>
 +
<br>
 +
This Gene is an <i>Escherichia coli</i> gene that has been shown to increase the yields of anthocyanin when paired with the genes in our anthocyanin construct. It is our second original basic part submission to the registry. It is uncharacterized and is an inter-membrane transport protein. We could not separate this gene form its composite part so we have added the nucleotide sequence to the registry
 
</p>
 
</p>
 
<br>
 
<br>
 
<br>
 
<br>
−
<h2><b>&nbsp;Composite Parts</b></h2>
 
  
−
<h3><b>Anthocyanin Construct</b></h3>
+
 
−
<p class="center"> This construct is being used to convert our initial molecule Eriodictyol into our final molecule Anthocyanin. The genes are:
+
<h1><b>&nbsp;Composite Parts</b></h1>
 +
<br>
 +
<div class="container-fluid" style="margin-left:10%; margin-right:10%;">
 +
<h2><b>&nbsp;Anthocyanin Constructs</b></h2>
 +
<br>
 +
<h3>Anthocyanin composite part 1</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281101</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<br>
 
<br>
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 +
<br>
 +
<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497010">BBa_K1497010</a>
 
<br>
 
<br>
 
<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497009">BBa_K1497009</a>
 
<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497009">BBa_K1497009</a>
 
<br>
 
<br>
−
<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497010">BBa_K1497010</a>
+
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 +
</p>
 +
<br>
 +
 
 +
<h3>Anthocyanin composite part 2</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281102</a>
 +
<br>
 +
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 +
<br>
 +
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
 
<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497002">BBa_K1497002</a>
 
<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497002">BBa_K1497002</a>
 
<br>
 
<br>
−
<b><i>3gt</i></b> Part BBa_waitingforsubmission
+
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 +
</p>
 +
<br>
 +
 
 +
<h3>Anthocyanin composite part 3</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281103</a>
 +
<br>
 +
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 +
<br>
 +
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 +
<br>
 +
<b><i>3gt</i></b> Part <a href="">BBa_K1281003</a>
 
<br>
 
<br>
−
<b><i>yad</i>H</b> Part BBa_alsowaiting
+
<b><i>yad</i>H</b> Part <a href="">BBa_K1281002</a>
 
<br>
 
<br>
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
These genes will all be used in the cell to produce Anthocyanin out of Eriodictyol. The <i>yad</i>H has been shown to increase yields even though it is not directly involved in the pathway. The <i>3gt</i> as well as <i>yad</i> H are two of our original basic part submissions. This Construct is our composite part submission BBa_anthocyanincompsitepart.
 
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/1/15/T--Lethbridge_HS--AnthocyaninPathway.png"></div>
+
These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/2/24/T--Lethbridge_HS--AnthocyaninConstruct.png"></div>
+
 
 +
 
 +
<div style="text-align:center;"><center><img height=50px class="img-responsive"src="https://static.igem.org/mediawiki/2017/1/15/T--Lethbridge_HS--AnthocyaninPathway.png"></center></div>
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/b/b4/T--Lethbridge_HS--yadHconstruct.png">
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/4/49/T--Lethbridge_HS--Anthocyanincomp1.png"></center></div>
 +
<br>
 +
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/9/9f/T--Lethbridge_HS--Anthocyanincomp2.png"></center>
 +
</div>
 +
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/0c/T--Lethbridge_HS--Anthocyanincomp3.png"></center>
 
</div>
 
</div>
 
</p>
 
</p>
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<br>
 
<br>
  
−
<h3><b>&nbsp;Zeaxanthin Construct</b></h3>
+
<h2><b>&nbsp;Zeaxanthin Construct</b></h2>
−
<p class="center"> This construct is made up of the following genes:
+
<h3>Zeaxanthin composite part</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281301</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
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<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
These parts all come together in our composite part <!-- link to the part--> BBa_Zeaxanthinconstruct. This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.
 
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/8/8f/T--Lethbridge_HS--ZeaxanthinPathway.png">
+
This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.
 +
<br>
 +
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/8/8f/T--Lethbridge_HS--ZeaxanthinPathway.png"></center>
 
</div>
 
</div>
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/c/c5/T--Lethbridge_HS--ZeaxanthinConstruct.png">
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/c/c5/T--Lethbridge_HS--ZeaxanthinConstruct.png"></center>
 
</div>
 
</div>
 
</p>
 
</p>
−
<br>
 
 
<br>
 
<br>
  
−
<h3><b>Indigoidine Construct</b></h3>
+
<h2><b>&nbsp;Indigoidine Constructs</b></h2>
−
<p class="center">Our Indigoidine construct is made up of:
+
<h3>Indigoidine composite part 1</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281201</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
Line 155: Line 206:
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<b><i>ind</i>B</b> Part <!--waiting for our part submission here-->BBa_waiting for actual part submission.
+
<b><i>ind</i>B</b> Part <a href="">BBa_K1281001</a>
 +
<br>
 +
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 +
</p>
 +
<br>
 +
 
 +
<h3>Indigoidine composite part 2</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281202</a>
 +
<br>
 +
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 +
<br>
 +
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
 
<b><i>ind</i>C</b> Part <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a>
 
<b><i>ind</i>C</b> Part <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a>
Line 161: Line 224:
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
These two genes come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is one of our original basic part submissions. This construct is part BBa_idkyet
 
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png">
+
These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. This construct is part <a href="">BBa_K1281201</a> and part <a href="">BBa_K1281202</a>. We had to split our genes into tow separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.
 +
<br>
 +
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png"></center>
 
</div>
 
</div>
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/2/25/T--Lethbridge_HS--IndigoidineConstruct.png">
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/2/25/T--Lethbridge_HS--IndigoidineConstruct.png"></center>
 
</div>
 
</div>
 
</p>  
 
</p>  
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−
<h3><b>Melanin Construct</b></h3>
+
<h2><b>&nbsp;Melanin Construct</b></h2>
 +
<h3>Melanin composite part</h3>
 
<p class="center">The parts used in our Melanin construct are as follows:
 
<p class="center">The parts used in our Melanin construct are as follows:
 
<br>
 
<br>
Line 184: Line 249:
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
This construct allows us to make Melanin out of L-Tyrosine. it is Part BBa_waitandsee
 
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/a/ad/T--Lethbridge_HS--MelaninPathway.png">
+
This construct allows us to make Melanin out of L-Tyrosine.
 +
<br>
 +
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/a/ad/T--Lethbridge_HS--MelaninPathway.png"></center>
 
</div>
 
</div>
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/f/f2/T--Lethbridge_HS--MelaninConstruct.png">
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/f/f2/T--Lethbridge_HS--MelaninConstruct.png"></center>
 
</div>
 
</div>
 
</p>
 
</p>
 
<br>
 
<br>
 
<br>
 
<br>
−
 
−
 
−
 
−
 
−
 
−
 
 
<br>
 
<br>
  

Revision as of 22:30, 26 October 2017





 Basic Parts


indBB

BBa_K1281001
This Gene is from the organism Petunia hybrid. It has been shwo to increase the yeilds of Indigoidine when paired with the gene indC. We could not separate this gene from its composite part so we have submitted the nucleotide sequence to the registry.



3gt

BBa_K281003
The gene 3gt is from the anthocyanin synthesis pathway and converts the initial molecule Pelargonidin into Anthocyanin. This gene is from the organism Petunia hybrid. We have added this part to the registry as one of our new basic part submissions. We could not get this gene out of its composite part so we added the sequence to the registry.

yadH

BBa_K1281002
This Gene is an Escherichia coli gene that has been shown to increase the yields of anthocyanin when paired with the genes in our anthocyanin construct. It is our second original basic part submission to the registry. It is uncharacterized and is an inter-membrane transport protein. We could not separate this gene form its composite part so we have added the nucleotide sequence to the registry



 Composite Parts


 Anthocyanin Constructs


Anthocyanin composite part 1

BBa_K1281101
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
dfr Part BBa_K1497010
f3h Part BBa_K1497009
Terminator Part BBa_B0015.


Anthocyanin composite part 2

BBa_K1281102
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
ans Part BBa_K1497002
Terminator Part BBa_B0015.


Anthocyanin composite part 3

BBa_K1281103
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
3gt Part BBa_K1281003
yadH Part BBa_K1281002
Terminator Part BBa_B0015.

These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted





 Zeaxanthin Construct

Zeaxanthin composite part

BBa_K1281301
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
crtY Part BBa_I742154.
crtZ Part BBa_I742157.
Terminator Part BBa_B0015.

This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.



 Indigoidine Constructs

Indigoidine composite part 1

BBa_K1281201
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indB Part BBa_K1281001
Terminator Part BBa_B0015.


Indigoidine composite part 2

BBa_K1281202
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indC Part BBa_K1152013
Terminator Part BBa_B0015.

These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The indB has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. This construct is part BBa_K1281201 and part BBa_K1281202. We had to split our genes into tow separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.




 Melanin Construct

Melanin composite part

The parts used in our Melanin construct are as follows:
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
MelA Part BBA_K274001.
Terminator Part BBa_B0015.

This construct allows us to make Melanin out of L-Tyrosine.