We are OUC_China,we come from Qingdao!

Qingdao is a beautiful city.However there are some annoying problems here.And that's what our team wants to solve this year.

 More at our Human Practice page!

Step 1

Now that we have plenty of Enteromorpha to deal with, we focus on the cellulose and hemicellulose which don’t have much added value in themselves thus wasn’t made good use of. And what we have done is fermentating them into ethyl alcohol. Co-culture system is one biological system which has a great potential, especially in the aspect of its cooperative fermentation. Fiber corpuscle, one of the scaffold protein, is an existing efficient system in the nature. But in yeast, its expression may make the host metabolic burden too heavy. So we think of using E. coli to replace fibre corpuscle, which plays a similar effect to increase the efficiency of the enzyme.

 More at our part1!

Step 2

After pretreatment, we successfully transformed the cellulose and hemicellulose into cellobiose and xylose that are easier to use. The cellobiose is then transformed into ethyl alcohol through the intrinsic pathway of saccharomyces cerevisiae . As for the xylose,we transform it into xylulose and ultimately the ethyl alcohol. Thus we created two kinds of functional yeast which can live on special carbon source ---when we feed them with cellobiose and xylose, they will produce ethyl alcohol in return. What’s more, we can adjust the ratio between two yeasts to accomplish the best output corresponding to the original composition of Enteromorpha. Amazing!

 More at our part2!

Step 3

In addition to Enteromorpha contains numerous cellulose and hemicellulose, there are a large number of antioxidant polysaccharide substances. These polysaccharide substances can be used as materials for health care products. At the same time, we use the yeast to express resveratrol, an antioxidant substances either, to improve our antioxidant health care products.

 More at our part3!

At this point,we have created Enteromorpha health care wine!

Step extra

When we compare the expression of eukaryotic and prokaryotic genes，eukaryotic gene expression vectors are larger because of single cistron，lengthy promoters and terminators.Therefore，we try to build a short and strong expression combination（mini-system）.It contains a small enough promoter and terminator.In addition, we tested to ensure optimal combination of promoter and terminator.

 More at our part4!