Difference between revisions of "Team:OUC-China"
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.igem_2017_menu_wrapper{display:none} | .igem_2017_menu_wrapper{display:none} | ||
.igem_2017_content_wrapper{width:100%;margin:0;float:none;font-size:17px;} | .igem_2017_content_wrapper{width:100%;margin:0;float:none;font-size:17px;} | ||
| − | + | .igem_2017_content_wrapper h1{display:none;} | |
.contentt{width: 100%;background: #0B486B;text-align: center;position: fixed;top: 0;margin: 0;padding: 0;} | .contentt{width: 100%;background: #0B486B;text-align: center;position: fixed;top: 0;margin: 0;padding: 0;} | ||
.contentt ul{display: inline-block;padding:0;} | .contentt ul{display: inline-block;padding:0;} | ||
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#wrap{margin: 0 auto;width: 1200px;padding: 0;} | #wrap{margin: 0 auto;width: 1200px;padding: 0;} | ||
.ab1{width: 100%;height: 450px;margin-bottom: 20px;margin-top: 80px;background-image:url(https://static.igem.org/mediawiki/2017/9/9a/T--OUC-China--ab1.jpeg);} | .ab1{width: 100%;height: 450px;margin-bottom: 20px;margin-top: 80px;background-image:url(https://static.igem.org/mediawiki/2017/9/9a/T--OUC-China--ab1.jpeg);} | ||
| − | .ab1 | + | .ab1 h2{padding-left: 420px;color:#79BD9A;font-size: 180%;padding-bottom:20px;} |
.ab1 p{width:800px;float: right;margin-bottom: 10px;} | .ab1 p{width:800px;float: right;margin-bottom: 10px;} | ||
.ab1 a{margin-left: 400px;text-decoration: none;color: #79BD9A;} | .ab1 a{margin-left: 400px;text-decoration: none;color: #79BD9A;} | ||
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.ab1 .vidio {width: 800px;height: 250px;background-color: gray;color: white;text-align: center;line-height: 250px;float: right;margin-top: 10px;} | .ab1 .vidio {width: 800px;height: 250px;background-color: gray;color: white;text-align: center;line-height: 250px;float: right;margin-top: 10px;} | ||
.ab2{width: 100%;height: 450px;background-image:url(https://static.igem.org/mediawiki/2017/e/ef/T--OUC-China--ab2.jpeg);margin-bottom: 20px;} | .ab2{width: 100%;height: 450px;background-image:url(https://static.igem.org/mediawiki/2017/e/ef/T--OUC-China--ab2.jpeg);margin-bottom: 20px;} | ||
| − | .ab2 | + | .ab2 h2{margin-left: 150px;padding-top: 30px;color:#79BD9A;font-size: 180%;} |
.ab2 p{width: 980px; margin-left: 150px;padding-top: 10px;padding-bottom: 5px;} | .ab2 p{width: 980px; margin-left: 150px;padding-top: 10px;padding-bottom: 5px;} | ||
.ab2 a{margin-left: 150px;text-decoration: none;color: #79BD9A;} | .ab2 a{margin-left: 150px;text-decoration: none;color: #79BD9A;} | ||
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.ab2 a:hover img{opacity: 1;transition: 0.5s;} | .ab2 a:hover img{opacity: 1;transition: 0.5s;} | ||
.ab3{width: 100%;height: 450px;background-image: url(https://static.igem.org/mediawiki/2017/7/76/T--OUC-China--ab3.jpeg);margin-bottom: 20px;} | .ab3{width: 100%;height: 450px;background-image: url(https://static.igem.org/mediawiki/2017/7/76/T--OUC-China--ab3.jpeg);margin-bottom: 20px;} | ||
| − | .ab3 | + | .ab3 h2{margin-left: 80px;padding-top: 30px;color:#79BD9A;font-size: 180%;} |
.ab3 p{width:1000px;padding-top: 20px;margin-left: 80px;padding-bottom: 20px;} | .ab3 p{width:1000px;padding-top: 20px;margin-left: 80px;padding-bottom: 20px;} | ||
.ab3 a{margin-left: 220px;text-decoration: none;color: #79BD9A;} | .ab3 a{margin-left: 220px;text-decoration: none;color: #79BD9A;} | ||
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.ab3 a:hover img{opacity: 1;transition: 0.5s;} | .ab3 a:hover img{opacity: 1;transition: 0.5s;} | ||
.ab4{width: 100%;height: 450px;background-image: url(https://static.igem.org/mediawiki/2017/c/c4/T--OUC-China--ab4.jpeg);} | .ab4{width: 100%;height: 450px;background-image: url(https://static.igem.org/mediawiki/2017/c/c4/T--OUC-China--ab4.jpeg);} | ||
| − | .ab4 | + | .ab4 h2{margin-left: 245px;padding-top: 30px;color:#79BD9A;font-size: 180%;} |
.ab4 p{width: 700px;margin-left: 245px;padding-top: 20px;padding-bottom: 20px;} | .ab4 p{width: 700px;margin-left: 245px;padding-top: 20px;padding-bottom: 20px;} | ||
.ab4 a{margin-left: 245px;text-decoration: none;color: #79BD9A;} | .ab4 a{margin-left: 245px;text-decoration: none;color: #79BD9A;} | ||
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.ab4 a:hover img{opacity: 1;transition: 0.5s;} | .ab4 a:hover img{opacity: 1;transition: 0.5s;} | ||
.ab5{width: 100%;height: 400px;background-image: url(https://static.igem.org/mediawiki/2017/c/c4/T--OUC-China--ab5.jpeg);} | .ab5{width: 100%;height: 400px;background-image: url(https://static.igem.org/mediawiki/2017/c/c4/T--OUC-China--ab5.jpeg);} | ||
| − | .ab5 | + | .ab5 h2{padding-top: 50px;color:#79BD9A;font-size: 180%;} |
.ab5 .wine{padding-top: 0;text-align: center;font-size: 150%;color:#266D96; } | .ab5 .wine{padding-top: 0;text-align: center;font-size: 150%;color:#266D96; } | ||
.ab5 p{width: 800px;padding-top: 20px;padding-bottom: 20px;} | .ab5 p{width: 800px;padding-top: 20px;padding-bottom: 20px;} | ||
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<div class="ab1"> | <div class="ab1"> | ||
| − | < | + | <h2>We are OUC_China,we come from Qingdao!</h2> |
<p>Qingdao is a beautiful city.However there are some annoying problems here.And that's what our team wants to solve this year.</p> | <p>Qingdao is a beautiful city.However there are some annoying problems here.And that's what our team wants to solve this year.</p> | ||
<a href="#"><img src="https://static.igem.org/mediawiki/2017/a/aa/T--OUC-China--jiantou.jpeg" width="30" height="30"/> More at our Human Practice page!</a> | <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/aa/T--OUC-China--jiantou.jpeg" width="30" height="30"/> More at our Human Practice page!</a> | ||
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</div> | </div> | ||
<div class="ab2"> | <div class="ab2"> | ||
| − | < | + | <h2>Step 1</h2> |
<p>Now that we have plenty of Enteromorpha to deal with, we focus on the cellulose and hemicellulose which don’t have much added value in themselves thus wasn’t made good use of. And what we have done is fermentating them into ethyl alcohol. | <p>Now that we have plenty of Enteromorpha to deal with, we focus on the cellulose and hemicellulose which don’t have much added value in themselves thus wasn’t made good use of. And what we have done is fermentating them into ethyl alcohol. | ||
Co-culture system is one biological system which has a great potential, especially in the aspect of its cooperative fermentation. Fiber corpuscle, one of the scaffold protein, is an existing efficient system in the nature. But in yeast, its expression may make the host metabolic burden too heavy. So we think of using E. coli to replace fibre corpuscle, which plays a similar effect to increase the efficiency of the enzyme. | Co-culture system is one biological system which has a great potential, especially in the aspect of its cooperative fermentation. Fiber corpuscle, one of the scaffold protein, is an existing efficient system in the nature. But in yeast, its expression may make the host metabolic burden too heavy. So we think of using E. coli to replace fibre corpuscle, which plays a similar effect to increase the efficiency of the enzyme. | ||
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</div> | </div> | ||
<div class="ab3"> | <div class="ab3"> | ||
| − | < | + | <h2>Step 2</h2> |
<p> | <p> | ||
After pretreatment, we successfully transformed the cellulose and hemicellulose into cellobiose and xylose that are easier to use. | After pretreatment, we successfully transformed the cellulose and hemicellulose into cellobiose and xylose that are easier to use. | ||
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</div> | </div> | ||
<div class="ab4"> | <div class="ab4"> | ||
| − | < | + | <h2>Step 3</h2> |
<p>In addition to Enteromorpha contains numerous cellulose and hemicellulose, there are a large number of antioxidant polysaccharide substances. These polysaccharide substances can be used as materials for health care products. At the same time, we use the yeast to express resveratrol, an antioxidant substances either, to improve our antioxidant health care products. | <p>In addition to Enteromorpha contains numerous cellulose and hemicellulose, there are a large number of antioxidant polysaccharide substances. These polysaccharide substances can be used as materials for health care products. At the same time, we use the yeast to express resveratrol, an antioxidant substances either, to improve our antioxidant health care products. | ||
</p> | </p> | ||
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<div class="ab5"> | <div class="ab5"> | ||
<h1 class="wine">At this point,we have created Enteromorpha health care wine!</h1> | <h1 class="wine">At this point,we have created Enteromorpha health care wine!</h1> | ||
| − | < | + | <h2>Step extra</h2> |
<p>When we compare the expression of eukaryotic and prokaryotic genes，eukaryotic gene expression vectors are larger because of single cistron，lengthy promoters and terminators.Therefore，we try to build a short and strong expression combination（mini-system）.It contains a small enough promoter and terminator.In addition, we tested to ensure optimal combination of promoter and terminator. | <p>When we compare the expression of eukaryotic and prokaryotic genes，eukaryotic gene expression vectors are larger because of single cistron，lengthy promoters and terminators.Therefore，we try to build a short and strong expression combination（mini-system）.It contains a small enough promoter and terminator.In addition, we tested to ensure optimal combination of promoter and terminator. | ||
</p> | </p> | ||
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<div id="zanzhu"> | <div id="zanzhu"> | ||
<img src="https://static.igem.org/mediawiki/2017/6/6f/T--OUC-China--zanzhu.jpeg" width=100%/> | <img src="https://static.igem.org/mediawiki/2017/6/6f/T--OUC-China--zanzhu.jpeg" width=100%/> | ||
| − | <p><span>Thanks: </span> | + | <p><span>Thanks: </span>（此处还是放图标吧)</p> |
| + | <p><span>Contact us: </span>oucigem@163.com<img src="https://static.igem.org/mediawiki/2017/b/b4/T--OUC-China--foot1.jpeg" width="50" height="50"/><img src="https://static.igem.org/mediawiki/2017/6/62/T--OUC-China--foot2.jpeg" width="50" height="50"></p> | ||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
Revision as of 03:24, 18 June 2017
We are OUC_China,we come from Qingdao!
Qingdao is a beautiful city.However there are some annoying problems here.And that's what our team wants to solve this year.
More at our Human Practice page!
此处是vidio
Step 1
Now that we have plenty of Enteromorpha to deal with, we focus on the cellulose and hemicellulose which don’t have much added value in themselves thus wasn’t made good use of. And what we have done is fermentating them into ethyl alcohol. Co-culture system is one biological system which has a great potential, especially in the aspect of its cooperative fermentation. Fiber corpuscle, one of the scaffold protein, is an existing efficient system in the nature. But in yeast, its expression may make the host metabolic burden too heavy. So we think of using E. coli to replace fibre corpuscle, which plays a similar effect to increase the efficiency of the enzyme.
More at our part1!
Step 2
After pretreatment, we successfully transformed the cellulose and hemicellulose into cellobiose and xylose that are easier to use. The cellobiose is then transformed into ethyl alcohol through the intrinsic pathway of saccharomyces cerevisiae . As for the xylose,we transform it into xylulose and ultimately the ethyl alcohol. Thus we created two kinds of functional yeast which can live on special carbon source ---when we feed them with cellobiose and xylose, they will produce ethyl alcohol in return. What’s more, we can adjust the ratio between two yeasts to accomplish the best output corresponding to the original composition of Enteromorpha. Amazing!
More at our part2!
Step 3
In addition to Enteromorpha contains numerous cellulose and hemicellulose, there are a large number of antioxidant polysaccharide substances. These polysaccharide substances can be used as materials for health care products. At the same time, we use the yeast to express resveratrol, an antioxidant substances either, to improve our antioxidant health care products.
More at our part3!
At this point,we have created Enteromorpha health care wine!
Step extra
When we compare the expression of eukaryotic and prokaryotic genes，eukaryotic gene expression vectors are larger because of single cistron，lengthy promoters and terminators.Therefore，we try to build a short and strong expression combination（mini-system）.It contains a small enough promoter and terminator.In addition, we tested to ensure optimal combination of promoter and terminator.
More at our part4!
Thanks: （此处还是放图标吧)
Contact us: oucigem@163.com
