Difference between revisions of "Team:Exeter/Lab Introduction"
| Line 41: | Line 41: | ||
<h5 id= "h1">Modified pili expression </h5> | <h5 id= "h1">Modified pili expression </h5> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<p>We wanted to produce modified pili to primarily bind a variety of metal ions but also with reporters to allow easy verification of protein expression. A two plasmid system was designed where the modified FimH would be expressed from one plasmid and the remaining proteins in the fim operon expressed from the second plasmid. The two plasmids carried compatible origins of replication (pUC and p15A) and different antibiotic resistance genes (AmpR and CmR) to allow for co-transformation in <i>E. coli</i> (Fig.1) </p> | <p>We wanted to produce modified pili to primarily bind a variety of metal ions but also with reporters to allow easy verification of protein expression. A two plasmid system was designed where the modified FimH would be expressed from one plasmid and the remaining proteins in the fim operon expressed from the second plasmid. The two plasmids carried compatible origins of replication (pUC and p15A) and different antibiotic resistance genes (AmpR and CmR) to allow for co-transformation in <i>E. coli</i> (Fig.1) </p> | ||
<img class="rounded mx-auto d-block w-50" src="https://2017.igem.org/File:T--Exeter--Two_Plasmids.png"> | <img class="rounded mx-auto d-block w-50" src="https://2017.igem.org/File:T--Exeter--Two_Plasmids.png"> | ||
</p> | </p> | ||
<figcaption>Fig.1 Plasmids</figcaption> | <figcaption>Fig.1 Plasmids</figcaption> | ||
| + | |||
| + | <h5 id="h2">Hydrocyclone </h5> | ||
Revision as of 16:11, 27 October 2017
Pili+
Modified pili expression
We wanted to produce modified pili to primarily bind a variety of metal ions but also with reporters to allow easy verification of protein expression. A two plasmid system was designed where the modified FimH would be expressed from one plasmid and the remaining proteins in the fim operon expressed from the second plasmid. The two plasmids carried compatible origins of replication (pUC and p15A) and different antibiotic resistance genes (AmpR and CmR) to allow for co-transformation in E. coli (Fig.1)
