Difference between revisions of "Team:Cadets2Vets/Experiments"

Line 2: Line 2:
 
<html>
 
<html>
  
−
<div class="column full_size">
+
<head>
 +
<title>
 +
EXPERIMENTS - CADETS2VETS</title>
 +
<!-- META -->
 +
<meta charset="UTF-8">
 +
<meta name="viewport" content="width=device-width, maximum-scale=1"/>
 +
 +
 +
<meta name="robots" content="noindex, nofollow">
 +
 +
 +
 +
<script src="https://www.youtube.com/iframe_api"></script>
 +
<!-- CSS -->
 +
<link rel="stylesheet" href="//edit.www.imcreator.com/css/common.css?v=1.4.0p1">
 +
<link rel="stylesheet" href="//edit.www.imcreator.com/js/lib/sweetalert/sweetalert.css" />
 +
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/all_css.css?v=1.4.0p1" />
  
−
<h1>Experiments</h1>
+
−
<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
+
 +
<link rel="icon" type="image/png" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" />
 +
<link rel="apple-touch-icon" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" />
 +
 +
 +
 +
<link rel="stylesheet" type="text/css" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css" />
 +
 +
<link id="style-c148c-oayvzg7x" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-c148c-oayvzg7x&viewer=xprs&parent_style=style-b1e6cf06-dzebo07f&v=1.4.0p1">
 +
<link id="style-c1c57ea5-gxbnxoxo" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-c1c57ea5-gxbnxoxo&viewer=xprs&parent_style=style-b1e6cf06-dzebo07f&v=1.4.0p1">
 +
<link id="style-75f14fec-vd1varn9" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-75f14fec-vd1varn9&viewer=xprs&parent_style=style-b1e6cf06-dzebo07f&v=1.4.0p1">
 +
<link id="style-fa7a1c05-5rrbvwct" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-fa7a1c05-5rrbvwct&viewer=xprs&parent_style=style-b1e6cf06-dzebo07f&v=1.4.0p1">
 +
<link id="style-c148c-zjadrzwq" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-c148c-zjadrzwq&viewer=xprs&parent_style=style-b1e6cf06-dzebo07f&v=1.4.0p1">
 +
<link id="style-b1e6cf06-dzebo07f" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-b1e6cf06-dzebo07f&viewer=xprs&parent_style=style-b1e6cf06-dzebo07f&v=1.4.0p1">
 +
<link id="style-c148c-ilf5hgbo" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-c148c-ilf5hgbo&viewer=xprs&parent_style=style-b1e6cf06-dzebo07f&v=1.4.0p1">
 +
<link id="style-493212c2-nib2cvub" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-493212c2-nib2cvub&viewer=xprs&parent_style=style-b1e6cf06-dzebo07f&v=1.4.0p1">
 +
<link id="vbid-b1e6cf06-lok1anrm-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_data_css?page_id=vbid-b1e6cf06-lok1anrm-STRIPE_DATA&v=1.4.0p1">
 +
 +
<link id="vbid-c148c-nxe9zty7-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_data_css?page_id=vbid-c148c-nxe9zty7-STRIPE_DATA&v=1.4.0p1">
 +
 +
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/fonts.css?v=1.4.0p1" />
 +
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/effects.css?v=1.4.0p1" />
 +
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/lightbox.css?v=1.4.0p1">
 +
 +
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/spimeview.css?v=1.4.0p1">
 +
 +
 +
<!-- SCRIPT -->
 +
<script src="https://code.jquery.com/jquery-2.x-git.min.js" type="text/javascript"></script>
 +
<script src="https://maps.googleapis.com/maps/api/js?key=AIzaSyByWRZWFbQRxJM7SiNz2NURPDPJPqTtBQQ" type="text/javascript"></script>
 +
 +
<script src="//edit.www.imcreator.com/js/xprs_helper.js?v=1.4.0p1" type="text/javascript"></script>
 +
 +
<script src="//edit.www.imcreator.com/js/lib/jquery.cookie.min.js" type="text/javascript"></script>
 +
<script src="//edit.www.imcreator.com/js/lib/sweetalert/sweetalert.min.js" type="text/javascript"></script>
 +
<script src="//edit.www.imcreator.com/translation_js?lang=en" type="text/javascript"></script>
 +
 +
<!-- User Head Code -->
 +
 +
 +
<script src="https://imos004-dot-im--os.appspot.com/js/imos.js?v=1.4.0p1" type="text/javascript"></script>
  
−
<p>
+
<script type="text/javascript" src="//edit.www.imcreator.com/all_js.js?v=1.4.0p1"></script>
−
Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.  
+
<script src="//edit.www.imcreator.com/js/lib/touchswipe/jquery.mobile.custom.min.js"></script>
−
</p>
+
<script type="text/javascript">IMOS.pageView();</script>
 +
 +
 +
 +
 +
<!-- User analytics -->
  
−
</div>
+
<script type="text/javascript">
 +
  var _gaq = _gaq || [];
 +
  _gaq.push(['_setAccount', '']);
 +
  _gaq.push(['_trackPageview']);
 +
  (function() {
 +
    var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true;
 +
    ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js';
 +
    var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s);
 +
  })();
 +
</script>
  
−
<div class="column half_size">
 
−
<h5>What should this page contain?</h5>
 
−
<ul>
 
−
<li> Protocols </li>
 
−
<li> Experiments </li>
 
−
<li> Documentation of the development of your project </li>
 
−
</ul>
 
  
−
</div>
+
<!-- Label config-->
 +
<script>
 +
 +
var LABEL_CONFIG = JSON.parse('{"LANDING_PAGE": "fast_homepage", "BILLING": {"BUY_NOW_URL": "none", "BUY_NOW_IMAGE": "none", "PAYPAL_RECURRENCES": "1", "CUSTOM_CURRENCY": "USD", "HIDE_FREE_URL": "off", "PAYPAL_ID": "", "PAYPAL_AMOUNT": "100", "CUSTOM_TITLE": "Single Commercial License", "PAYPAL_PERIOD": "Y", "CUSTOM_EXPLAINED": "single domain connection for a single site. unlimited bandwidth, unlimited storage, premium support, e-commerce", "BILLING_TYPE": "abtest", "PAYPAL_OFFER_NAME": "Premium Subscription", "PAYPAL_EXPLAINED": "single domain connection for a single site. unlimited bandwidth, unlimited storage, premium support, e-commerce", "CUSTOM_CYCLE": "/month paid annualy", "PAYPAL_CURRENCY": "USD", "CUSTOM_PRICE": "100"}, "BRANDING": {"BRAND_THEMES_LOGO": "none", "BRAND_SECONDARY_COLOR": "rgb(255,255,255)", "BRAND_MAIN_COLOR": "#0099CC", "BRAND_BG": "https://lh3.googleusercontent.com/ZgLp2jeL7ynJJZ-aMv-LgcFLv1-wuV0E4mMxsQXqyPe9CDHiClIlQODrGLalwzQp4XNWjMBy8Z68369P4Q", "BUTTON_HOVER_COLOR": "#0099cc", "BRAND_SMALL_LOGO": "https://lh3.googleusercontent.com/PVw5qfegr3TcOhyx6UH2QShnmtTexxEIuzECuxiKnbfkzYPv28lrzSMSnelUe7nkoYjs-KLxOYzo1wgOSZk", "BRAND_LARGE_LOGO": "https://lh3.googleusercontent.com/BVPCuhW88_eSWMXpfFE8ZZh9IxRktAzfOp_rZ9vvhH2zLhLNoCJ6TjOleEnqppMxvYHdvsxDX-uXWfRrgQ"}, "SUPPORT": {"SUPPORT_URL": "http://support.imxprs.com/hc/en-us/requests/new", "VIDEO_ID": "K1ZnSLe-Rdk", "SUPPORT_ID": "", "KNOWLEDGE_CENTER_URL": "http://support.imxprs.com/hc/en-us"}, "ANALYTICS_ID": "UA-64970204-1", "META": {"OWNER": "xprsxprs", "DOMAIN": "imcreator.com", "CREATION_DATE": "28-07-2015 13:34:42", "PRODUCT_NAME": "XPRS", "NAME": "xprs"}, "DOMAIN_BILLING": {"BUY_NOW_URL": "none", "PAYPAL_RECURRENCES": "1", "CUSTOM_CURRENCY": "USD", "PAYPAL_PERIOD": "Y", "PAYPAL_AMOUNT": "100", "PAYPAL_ID": "", "CUSTOM_EXPLAINED": "1-Year domain subscription", "BILLING_TYPE": "standard", "PAYPAL_OFFER_NAME": "Premium Subscription", "PAYPAL_CURRENCY": "USD", "CUSTOM_PRICE": "100"}, "ID": "label-f6d7c", "SETTINGS": {"ALLOW_PERIPHERAL_PAGES": "allow", "LOGIN_URL": "", "ALLOW_ECOMMERCE": "allow", "ALLOW_NEW_DOMAIN": "allow", "ENABLE_IMXPRT": "true", "ECOMMERCE_DASHBOARD": "http://dashboard.shoprocket.co", "INCLUDE_REGISTER_PHONE": "disallow", "CUSTOM_TEMPLATES": "default", "API_TOKEN": "NOT GENERATED", "ALLOW_CALL_ME_CHECKBOX": "disallow", "SR_PARTNER_KEY": "N/A", "PHONE_EDIT_APP_LINK": "https://itunes.apple.com/app/apple-store/id953999749?pt=1847141&mt=8", "DEFAULT_EDITOR_LANG": "en", "CUSTOM_SECTIONS": "default", "ALLOW_PHONE_EDIT": "allow", "ALLOW_NEW_SITES": "allow", "REGISTER_URL": "", "BILLING_SERVER": "", "USER_PROFILE": "show", "REGISTER_STEP": "themes", "ENABLE_IMOS": "false", "ECOMMERCE_SOLUTION": "IMOS", "WEBHOOKS_SERVER": "", "ALLOW_RESELLER": "allow"}, "HIY": "false", "EMAILS": {"FORWARD_ALL_EMAILS": "off", "FORWARD_EMAIL": ""}, "SERVERS": {"HEROKU_APP": "", "S3_ACCESS": "", "S3_REGION": "", "UPLOAD_TO_S3": "off", "S3_SECRET": "", "S3_BUCKET": ""}, "TOS_URL": "http://www.imcreator.com/terms-of-service"}');
 +
 +
</script></head>
  
−
<div class="column half_size">
+
<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-c148c-nxe9zty7" data-root-style-id="style-c148c-ilf5hgbo" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.0p1" data-caller="preview"  data-ecommerce-dashboard="http://dashboard.shoprocket.co" data-static-server="//edit.www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
−
<h5>Inspiration</h5>
+
−
<ul>
+
<div id="xprs" data-website-name="CADETS2VETS" class="xprs-holder" >
−
<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
+
<div class="main-page "  >
−
<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
+
<div id="content">
−
<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
+
<div  id="vbid-b1e6cf06-lok1anrm"  class="master container style-b1e6cf06-dzebo07f content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="EXPERIMENTS"  data-itemslug="experiments" data-itemstyleid="style-b1e6cf06-dzebo07f" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-b1e6cf06-lok1anrm" data-preset-type-id="ITEMS" data-preview-style="style-b1e6cf06-dzebo07f" data-style="style-5a24e-kzdyurm3ju" data-absolute-path="http://edit.www.imcreator.com/viewer" >
−
</ul>
+
<!-- ARRANGER SETTINGS -->
−
</div>
+
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="stripes"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="middle"></div>
 +
<div id="children">
 +
 +
 +
 +
<!-- MENUS START -->
 +
<div  id="vbid-c148c-zyed6lpc"  class="master item-box  header-box style-c148c-oayvzg7x injected        " data-holder-type="header"" data-holder-type="header"  data-child-type="ORIGIN"  data-styleid="style-c148c-oayvzg7x" data-preview-styleid='style-c148c-oayvzg7x' data-preset-type-id="MENUS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="header-wrapper item-wrapper menus-wrapper ">
 +
 +
<div class="item-content leaf menu_layout header content" data-preview-style="style-c148c-oayvzg7x" data-style="style-7f715-scjjarqgxu"  data-behavior-type="NOTHING" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-c148c-zyed6lpc" data-bgimg="">
 +
<div class="preview-content-wrapper">
 +
<div class="preview-content-holder">
 +
<div class="left-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<div class="logo-holder">
 +
<!-- ICON TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
  
 +
</div>
 +
<div class="helper-div">
 +
<div class="item-details menu">
 +
<!-- TITLE TEMPLATE -->
 +
 +
<div class="preview-title-holder removable-parent">
 +
 +
<h2 id="vbid-c148c-kbardskx" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Menu</h2>
 +
 +
</div>
 +
<br />
  
−
<div class="clear"></div>
+
<!-- SUBTITLE TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
  
 +
</div>
 +
</div>
 +
</div>
 +
<div class="right-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<!-- LINKS TEMPLATE -->
 +
<div class="preview-item-links ">
 +
<ul class="preview-links-wrapper">
  
−
<div class="column half_size">
 
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-c148c-nxe9zty7/vbid-c148c-nucgmpxt" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-c148c-dh99iba8"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team Members</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-c148c-nxe9zty7/vbid-c148c-b2umwcrd" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-c148c-nohjnosa"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Projects</span>
 +
</a>
 +
 +
</li>
  
−
</div>
+
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-c148c-nxe9zty7/vbid-c148c-pz7ryxgi" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-c148c-brjwrteh"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-c148c-nxe9zty7/vbid-c148c-oxtsgzi2" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-c148c-r5jljoed"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-c148c-nxe9zty7/vbid-c148c-4azd86zb" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-c148c-qtoldy1c"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Human Practices</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-c148c-nxe9zty7/vbid-c148c-adfw6gge" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-c148c-faghxoxn"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Awards</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-c148c-nxe9zty7/vbid-c148c-sbqepono" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-c148c-rltjqwln"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Judging Form</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-c148c-nxe9zty7" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-c148c-8wbe3tkq"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-c148c-nxe9zty7/vbid-c148c-8mcnamkh" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-c148c-gfcb5lfe"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Collaborations</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-c148c-nxe9zty7/vbid-b1e6cf06-lok1anrm" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-18e22254017160d"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Experiments</span>
 +
</a>
 +
 +
</li>
  
  
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</ul>
 +
</div>
 +
 +
 +
<button class="hamburger links-menu-btn  small" type="button">
 +
  <span class="hamburger-box">
 +
  <span class="hamburger-inner"></span>
 +
  </span>
 +
</button>
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="menu"
 +
data-MENU_OVERLAY="relative"
 +
data-MENU_SCROLL="false"
 +
data-ALWAYS_MINIFIED="false"
 +
data-MENU_POSITION="top"
 +
data-MENU_ALIGN="left"
 +
data-BACKGROUND_COLOR="rgb(124, 100, 41)"
 +
></div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- MENUS END -->
 +
 +
 +
 +
 +
<!-- PROMO START -->
 +
<div  id="vbid-fa7a1c05-hpxy6gyb"  class="master item-box  gallery-box style-fa7a1c05-5rrbvwct          " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-fa7a1c05-5rrbvwct" data-preview-styleid='style-fa7a1c05-5rrbvwct' data-preset-type-id="PROMO">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper promo-wrapper ">
 +
 +
<!-- CACHED VERSION (7) OF vbid-fa7a1c05-hpxy6gyb FROM  19-06-2017 22:37:44 --><div  class="sub container style-fa7a1c05-5rrbvwct content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-fa7a1c05-5rrbvwct" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-fa7a1c05-hpxy6gyb" data-preset-type-id="PROMO" data-preview-style="style-fa7a1c05-5rrbvwct" data-style="style-89a8e-bbtqrmxrkr" data-absolute-path="http://edit.www.imcreator.com/viewer" >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-fa7a1c05-xn6zn1cw"  class="sub item-box  page-box style-fa7a1c05-5rrbvwct          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-fa7a1c05-5rrbvwct" data-preview-styleid='style-fa7a1c05-5rrbvwct' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-fa7a1c05-xn6zn1cw" data-preview-style="style-fa7a1c05-5rrbvwct" data-style="style-89a8e-q4q4gr5nhr" data-orig-thumb-height="504" data-orig-thumb-width="600" data-vbid="vbid-fa7a1c05-xn6zn1cw" data-bgimg="https://lh3.googleusercontent.com/eWaGqkBdwsI8pTfu-rzx3x3--TOAN6NYqmRcGlI9_shvW82ZIctRd_ZA0f0wNG1JHFPzJZsI9CpGY44PNEQ">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-fa7a1c05-afhcgh6b-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
 +
<div  id="vbid-fa7a1c05-afhcgh6b" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/eWaGqkBdwsI8pTfu-rzx3x3--TOAN6NYqmRcGlI9_shvW82ZIctRd_ZA0f0wNG1JHFPzJZsI9CpGY44PNEQ=s300);"  data-orig-width="600" data-orig-height="504" >
 +
<!-- VIDEO TEMPLATE -->
 +
  
−
<style>
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
−
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
+
  
−
:not(.navmenubkg){
 
−
  font-family:'Open Sans';
 
−
}
 
−
.navmenubkg {
 
−
font-family:'Roboto';
 
  
−
}
+
<!-- MAP TEMPLATE -->
−
h1,h2,h3,h4,h5,h6{
+
−
  font-family:'Roboto';
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
}
+
  
−
#top-section{
+
<!-- RAW TEMPLATE -->
−
width:100%;
+
−
margin-left:0;
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
left:0;
+
−
margin-bottom:5px;
+
−
}
+
  
−
.well{min-height:20px;padding:19px;margin-bottom:20px;background-color:#f5f5f5;border:1px solid #e3e3e3;border-radius:4px;-webkit-box-shadow:inset 0 1px 1px rgba(0,0,0,.05);box-shadow:inset 0 1px 1px rgba(0,0,0,.05)}
+
</div>
 +
 +
</div>
 +
  
−
td {
+
</div>
−
  text-align: justified;
+
</div>
−
  vertical-align: middle;
+
<div class="text-side shrinker-parent">
−
}
+
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h1 id="vbid-fa7a1c05-t4aecjep" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >EXPERIMENTS.<br></h1>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
−
tr{
+
−
width:1100px;
+
−
}
+
−
.quarters img, .quarters .well{
+
−
width:250px;
+
−
margin-left:auto;
+
−
margin-right:auto;
+
<br class="upper-line-break" />
−
padding-left:0;
+
<div class="preview-subtitle-holder removable-parent order-handle">
−
padding-right:0;
+
 +
<h3 id="vbid-fa7a1c05-gy05blag" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">RESEARCH. PROTOCOLS. </h3>
  
−
}
+
</div>
 +
<br class="lower-line-break" />
  
−
.triples img, .triples .well{
+
−
width:366px;
+
−
margin-left:auto;
+
−
margin-right:auto;
+
−
padding-left:0;
+
</div>
−
padding-right:0;
+
</div>
−
}
+
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- PROMO END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-75f14fec-lw9d5f4w"  class="master item-box  gallery-box style-75f14fec-vd1varn9          " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-75f14fec-vd1varn9" data-preview-styleid='style-75f14fec-vd1varn9' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<!-- CACHED VERSION (7) OF vbid-75f14fec-lw9d5f4w FROM  19-06-2017 22:37:44 --><div  class="sub container style-75f14fec-vd1varn9 content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-75f14fec-vd1varn9" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-75f14fec-lw9d5f4w" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-75f14fec-vd1varn9" data-style="style-4e411-j0xhjgzynh" data-absolute-path="http://edit.www.imcreator.com/viewer" >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-75f14fec-6ianhvhd"  class="sub item-box  page-box style-75f14fec-vd1varn9          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-75f14fec-vd1varn9" data-preview-styleid='style-75f14fec-vd1varn9' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-75f14fec-6ianhvhd" data-preview-style="style-75f14fec-vd1varn9" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="2304" data-orig-thumb-width="3456" data-vbid="vbid-75f14fec-6ianhvhd" data-bgimg="https://lh3.googleusercontent.com/ZqSjORLqOP-nzCnU_vSJeH_TYfPpnclPIFRMDXQeQcgmSKqupebgtFP-XJDyei66npxCRdMw994_aj2N">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="element-002b52ae266d9f9-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
.quarters .well,.triples .well{
+
<div  id="element-002b52ae266d9f9" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/ZqSjORLqOP-nzCnU_vSJeH_TYfPpnclPIFRMDXQeQcgmSKqupebgtFP-XJDyei66npxCRdMw994_aj2N=s300);"  data-orig-width="3456" data-orig-height="2304" >
−
margin-bottom:10px;
+
<!-- VIDEO TEMPLATE -->
−
padding:7px 0px;
+
−
font-size: 18px;
+
  
−
}
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
−
.quarters td{
 
−
width:250px;
 
  
−
}
+
<!-- MAP TEMPLATE -->
−
.triples td{
+
−
width:366px;
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
}
+
−
h1{
+
−
border-bottom:none;
+
−
margin-bottom:30px;
+
−
}
+
  
−
.callout{
+
<!-- RAW TEMPLATE -->
−
    font-family:'Open Sans';
+
−
    font-weight:700;
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
    color:#FFF;
+
−
    background-color:#213463;
+
−
    text-align:center;
+
−
    border-radius:4px;
+
−
    padding-top: 5px;
+
−
    padding-bottom: 5px;
+
−
}
+
  
−
.callout h1{
+
</div>
−
    color:#EEE;
+
−
}
+
</div>
 +
  
−
.tinycall{
+
</div>
−
    font-family:'Open Sans';
+
</div>
−
    font-weight:700;
+
<div class="text-side shrinker-parent">
−
    color:#213463;
+
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="vbid-75f14fec-gh7ewxmq" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">TICKETED CELL FREE EXPRESSION SYSTEM PROTOCOLS</h3>
  
−
    text-align:center;
+
</div>
−
    border-radius:2px;
+
<br class="lower-line-break" />
−
}
+
−
.tinycall h1{
+
−
color:#213463;
+
−
margin-top:0px;
+
−
margin-bottom:25px;
+
−
font-size:35px
+
−
}
+
−
}
+
−
</style>
+
  
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-75f14fec-plihujvt" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:38.4pt;text-indent:-38.4pt;mso-text-indent-alt:-.25in;line-height:
 +
18.0pt;mso-list:l0 level1 lfo1;tab-stops:list .5in"><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Open Sans&quot;;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span>I.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span></span><b><span style="font-size:10.0pt;font-family:
 +
&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;color:black">Overview</span></b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black"><o:p></o:p></span></p><p class="MsoNormal" style="margin: 4.8pt 0in 6pt 0.8in; line-height: 18pt;"><span style="font-size:10.0pt;font-family:
 +
&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;color:black">The idea of cell free expression has existed for more than 20 years. It is the notion of taking the elements required for both transcription and translation and utilizing them for an external protein expression system. This is extremely beneficial for expression of cytotoxic proteins in which high yields are difficult to produce in vivo. For our purpose within this assay, this concept is utilized in conjunction with a regulatory factor for detection. This is detection is predicated either by regulation of the RBS at the transcript level or by sequestering the binding sight of RNA polymerase.<o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:38.4pt;text-indent:-38.4pt;mso-text-indent-alt:-.25in;line-height:
 +
18.0pt;mso-list:l0 level1 lfo1;tab-stops:list .5in"><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Open Sans&quot;;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">        </span>II.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span></span><b><span style="font-size:10.0pt;font-family:
 +
&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;color:black">Work Flow</span></b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;
 +
mso-fareast-font-family:&quot;Times New Roman&quot;;color:black"><o:p></o:p></span></p><p class="MsoNormal" style="margin-bottom: 1.2pt; margin-left: 0.8in; text-indent: -0.8in; line-height: 18pt;"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">Identify trigger sequence and target plasmid or probe<o:p></o:p></span></p><p class="MsoNormal" style="margin-bottom: 1.2pt; margin-left: 0.8in; text-indent: -0.8in; line-height: 18pt;"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">Add target<o:p></o:p></span></p><p class="MsoNormal" style="margin-bottom: 1.2pt; margin-left: 0.8in; text-indent: -0.8in; line-height: 18pt;"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">Incubation<o:p></o:p></span></p><p class="MsoNormal" style="margin-bottom: 1.2pt; margin-left: 0.8in; text-indent: -0.8in; line-height: 18pt;"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">Evaluation<o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:38.4pt;text-indent:-38.4pt;mso-text-indent-alt:-.25in;line-height:
 +
18.0pt;mso-list:l0 level1 lfo1;tab-stops:list .5in"><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Open Sans&quot;;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>III.<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span></span><b><span style="font-size:10.0pt;font-family:
 +
&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;color:black">Reagents</span></b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black"><o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">Cell free expression kit<o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">•Promega L5540<o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">•Promega L1020<o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">•Promega L1110<o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">•NEB E6800L<o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">•Life Tech K990100<o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">RNASE Inhibitor (RI)<o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">Prepared tickets<o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">Trigger Plasmid<o:p></o:p></span></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;color:black"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;;color:black">Substrate if needed<o:p></o:p></span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-493212c2-kxacqlds"  class="master item-box  gallery-box style-493212c2-nib2cvub          " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-493212c2-nib2cvub" data-preview-styleid='style-493212c2-nib2cvub' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<!-- CACHED VERSION (7) OF vbid-493212c2-kxacqlds FROM  19-06-2017 22:37:45 --><div  class="sub container style-493212c2-nib2cvub content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-493212c2-nib2cvub" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-493212c2-kxacqlds" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-493212c2-nib2cvub" data-style="style-89a8e-bbtqrmxrkr" data-absolute-path="http://edit.www.imcreator.com/viewer" >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-493212c2-pu8fpxsk"  class="sub item-box  page-box style-493212c2-nib2cvub          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-493212c2-nib2cvub" data-preview-styleid='style-493212c2-nib2cvub' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-493212c2-pu8fpxsk" data-preview-style="style-493212c2-nib2cvub" data-style="style-89a8e-q4q4gr5nhr" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-493212c2-pu8fpxsk" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
 +
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
  
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
  
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
  
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
  
 +
</div>
 +
 +
</div>
 +
  
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="vbid-493212c2-hlawibyw" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">aPCR- Quantitative Polymerase Chain Reaction Protocol<br></h3>
  
 +
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-divider-holder removable-parent order-handle">
 +
<div id="vbid-493212c2-cyzjxrwl" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 +
</div>
 +
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="element-07a7ee566a31b20" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed malesuada faucibus ex nec ultricies. Donec mattis egestas nisi non pretium. Suspendisse nec eros ut erat facilisis maximus. In congue et leo in varius. Vestibulum sit amet felis ornare, commodo orci ut, feugiat lorem.
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="element-07a7ee566a31b20" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed malesuada faucibus ex nec ultricies. Donec mattis egestas nisi non pretium. Suspendisse nec eros ut erat facilisis maximus. In congue et leo in varius. Vestibulum sit amet felis ornare, commodo orci ut, feugiat lorem.
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-c1c57ea5-v8dtraty"  class="master item-box  gallery-box style-c1c57ea5-gxbnxoxo    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-c1c57ea5-gxbnxoxo" data-preview-styleid='style-c1c57ea5-gxbnxoxo' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<!-- CACHED VERSION (7) OF vbid-c1c57ea5-v8dtraty FROM  19-06-2017 22:37:45 --><div  class="sub container style-c1c57ea5-gxbnxoxo content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-c1c57ea5-gxbnxoxo" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-c1c57ea5-v8dtraty" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-c1c57ea5-gxbnxoxo" data-style="style-4e411-j0xhjgzynh" data-absolute-path="http://edit.www.imcreator.com/viewer" >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-c1c57ea5-inze0asn"  class="sub item-box  page-box style-c1c57ea5-gxbnxoxo          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-c1c57ea5-gxbnxoxo" data-preview-styleid='style-c1c57ea5-gxbnxoxo' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-c1c57ea5-inze0asn" data-preview-style="style-c1c57ea5-gxbnxoxo" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="4857" data-orig-thumb-width="1600" data-vbid="vbid-c1c57ea5-inze0asn" data-bgimg="https://lh3.googleusercontent.com/RA5j0HepG9cF2_DaVUMd8S32ljZZEzWqXazA4vFzyDl2Xg5M2IMl_sChycAlLSdh0uKPcWp974Yw-MM">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-left" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-c1c57ea5-g2odh1bs-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
 +
<div  id="vbid-c1c57ea5-g2odh1bs" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/RA5j0HepG9cF2_DaVUMd8S32ljZZEzWqXazA4vFzyDl2Xg5M2IMl_sChycAlLSdh0uKPcWp974Yw-MM=s300);"  data-orig-width="1600" data-orig-height="4857" >
 +
<!-- VIDEO TEMPLATE -->
 +
  
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
  
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
  
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
  
 +
</div>
 +
 +
</div>
 +
  
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-c1c57ea5-wtwxjk0y" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Experimental outline<br></h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-divider-holder removable-parent order-handle">
 +
<div id="vbid-c1c57ea5-5k3wmr7v" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 +
</div>
 +
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-c1c57ea5-0uefpxyr" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:38.4pt;text-indent:-38.4pt;mso-text-indent-alt:-.25in;line-height:
 +
18.0pt;mso-list:l0 level1 lfo1;tab-stops:list .5in"><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Open Sans&quot;"><span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span>I.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span></span></b><b><span style="font-size:10.0pt;font-family:
 +
&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;">Pre-Phase One Experiments<o:p></o:p></span></b></p><p class="MsoNormal" style="margin-top:4.8pt;margin-right:0in;margin-bottom:6.0pt;
 +
margin-left:.8in;line-height:18.0pt"><b><span style="font-size:10.0pt;
 +
font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;">Establish control experiments<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">-Constitutive GFP<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">o Switch H GFP<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:38.4pt;text-indent:-38.4pt;mso-text-indent-alt:-.25in;line-height:
 +
18.0pt;mso-list:l0 level1 lfo1;tab-stops:list .5in"><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Open Sans&quot;"><span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">        </span>II.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span></span></b><b><span style="font-size:10.0pt;font-family:
 +
&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;">Pre-Phase One Goals<o:p></o:p></span></b></p><p class="MsoNormal" style="margin-bottom: 1.2pt; margin-left: 0.8in; text-indent: -0.8in; line-height: 18pt;"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">The goal of pre-phase 1 is to establish a working positive control and negative control in lab.<o:p></o:p></span></b></p><p class="MsoNormal" style="margin-bottom: 1.2pt; margin-left: 38.4pt; text-indent: -38.4pt; line-height: 18pt;"><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Open Sans&quot;"><span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>III.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span></span></b><b><span style="font-size:10.0pt;font-family:
 +
&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;">Phase One Experiments<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">Clone composite circuit in e.coli<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">Test efficacy of the circuit in vivo and determine threshold limits of both arsenic and arsenite<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">Perform mini prep and quantify<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:38.4pt;text-indent:-38.4pt;mso-text-indent-alt:-.25in;line-height:
 +
18.0pt;mso-list:l0 level1 lfo1;tab-stops:list .5in"><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Open Sans&quot;"><span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>IV.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span></span></b><b><span style="font-size:10.0pt;font-family:
 +
&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;">Phase One Goals<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">The goals of this phase is to grow a large amount of the composite circuit and develop an understanding of whether or not the circuit is biologically effective. Another primary goal is to develop an understanding of how the circuit works in its entirety (upper threshold/lower threshold)<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:38.4pt;text-indent:-38.4pt;mso-text-indent-alt:-.25in;line-height:
 +
18.0pt;mso-list:l0 level1 lfo1;tab-stops:list .5in"><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Open Sans&quot;"><span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">        </span>V.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span></span></b><b><span style="font-size:10.0pt;font-family:
 +
&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;">Phase Two Experiments<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">Test the efficacy of the composite circuit instead of crude lysate<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">Test purified plasmid with Promega S30 kit<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:38.4pt;text-indent:-38.4pt;mso-text-indent-alt:-.25in;line-height:
 +
18.0pt;mso-list:l0 level1 lfo1;tab-stops:list .5in"><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Open Sans&quot;"><span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>VI.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span></span></b><b><span style="font-size:10.0pt;font-family:
 +
&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;">Phase Two Goals<o:p></o:p></span></b></p><p class="MsoNormal" style="margin-bottom: 1.2pt; margin-left: 0.8in; text-indent: -0.8in; line-height: 18pt;"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">The goal of this phase is to take the circuit out of the cell and test it in situ utilizing both a bacterial expression kit (Promega S30) and an attempt at functionalizing the circuit utilizing a formulated crude lysate for increased application space.<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:38.4pt;text-indent:-38.4pt;mso-text-indent-alt:-.25in;line-height:
 +
18.0pt;mso-list:l0 level1 lfo1;tab-stops:list .5in"><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Open Sans&quot;"><span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">    </span>VII.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span></span></b><b><span style="font-size:10.0pt;font-family:
 +
&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;">Phase Three Experiments<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">Attempt at micro scaling reaction<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">Porting reactions to paper based system<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:.8in;text-indent:-.8in;mso-text-indent-alt:-.25in;line-height:18.0pt;
 +
mso-list:l0 level2 lfo1;tab-stops:list 1.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">      </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">Attempt at lyophilizing the circuit on paper both with Promega S30 kit and in the crude lysate form (if the previous phase demonstrated reasonable evidence for progressing on that front)<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:76.8pt;text-indent:-76.8pt;mso-text-indent-alt:-.25in;line-height:
 +
18.0pt;mso-list:l0 level3 lfo1;tab-stops:list 1.5in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">        </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">Phase Three Goals<o:p></o:p></span></b></p><p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 +
margin-left:96.0pt;text-indent:-96.0pt;mso-text-indent-alt:-.25in;line-height:
 +
18.0pt;mso-list:l0 level4 lfo1;tab-stops:list 2.0in"><span style="font-size:10.0pt;font-family:Wingdings;mso-fareast-font-family:Wingdings;
 +
mso-bidi-font-family:Wingdings;mso-bidi-font-weight:bold"><span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">          </span>§<span style="font-variant-numeric: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: &quot;Times New Roman&quot;;">  </span></span><b><span style="font-size:10.0pt;font-family:&quot;Open Sans&quot;,&quot;sans-serif&quot;;mso-fareast-font-family:
 +
&quot;Times New Roman&quot;">The goal of this phase is to successfully micro scale the reaction and port it to a lateral flow paper based system. If successful in this phase, we will have developed a biological means to detect presence of arsenic and arsenite utilizing a lateral flow paper system that is both cost effective and easy to implement.<o:p></o:p></span></b></p><p class="MsoNormal"><o:p> </o:p></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- FOOTERS START -->
 +
<div  id="vbid-c148c-ybb2oqrd"  class="master item-box  footer-box style-c148c-zjadrzwq injected        " data-holder-type="footer"" data-holder-type="footer"  data-child-type="ORIGIN"  data-styleid="style-c148c-zjadrzwq" data-preview-styleid='style-c148c-zjadrzwq' data-preset-type-id="FOOTERS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="footer-wrapper item-wrapper footers-wrapper ">
 +
 +
<div class="item-content leaf middle_layout footer content shrinker-parent" data-self="vbid-c148c-ybb2oqrd" data-preview-style="style-c148c-zjadrzwq" data-style="style-f785e-jp5ywgaa0t" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-c148c-ybb2oqrd" data-bgimg="">
 +
<div  class="preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
 +
<div  id="no-image" data-title='click to Add'  class="background-div  normal preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="PREVIEW_IMAGE" style="" >
 +
<!-- VIDEO TEMPLATE -->
 +
  
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
  
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
  
 +
</div>
 +
<div class="helper-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<div class="item-details preview-content-wrapper  middle">
 +
<div class="preview-content-holder shrinker-content">
 +
<!-- ICON TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
  
 +
<!-- TITLE TEMPLATE -->
 +
 +
<div class="preview-title-holder removable-parent">
 +
 +
<h2 id="vbid-c148c-qa354hdh" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Follow us on Social Media!</h2>
 +
 +
</div>
 +
<br />
  
 +
<!-- SUBTITLE TEMPLATE -->
 +
 +
<div class="preview-subtitle-holder removable-parent">
 +
 +
<h3 id="vbid-c148c-mxkafou0" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">2304 Jefferson Ave, Tacoma, WA 98402</h3>
  
−
<!--main content -->
+
</div>
−
<table width="1100px" align="center" style="margin-top:0px">
+
<br />
−
<!--welcome box -->
+
−
<tr>
+
−
<td>
+
−
<div style="margin-top:50px"> </div>
+
−
<div class="callout">
+
−
<h1 style="margin-top:22px; font-size:50px;">Experiments</h1>
+
−
</td>
+
−
</tr>
+
−
<!-- end welcome box -->
+
−
 
+
−
<tr><td colspan="4" height="5px"></td> </tr>
+
−
 
+
−
<tr><td colspan="4" height="15px"></td> </tr>
+
  
 +
<!-- BODY TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
  
−
<td colspan="12">
+
<!-- PRICE TEMPLATE -->
−
<h1 style="margin-top:15px; margin-bottom:25px; font-size:35px;text-align:center">Ticketed Cell Free Expression System Protocol</h1>
+
−
<div class = "well">
+
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
−
<p>
+
−
<ol type="I"><li><strong>Overview</strong><ul style="list-style-type:square">  
+
−
</p>
+
−
<p>
+
−
    The idea of cell free expression has existed for more than 20 years. It is the notion of taking the elements required for both transcription and translation and utilizing them for an external protein expression system. This is extremely beneficial for expression of cytotoxic proteins in which high yields are difficult to produce in vivo. For our purpose within this assay, this concept is utilized in conjunction with a regulatory factor for detection. This is detection is predicated either by regulation of the RBS at the transcript level or by sequestering the binding sight of RNA polymerase. </p></ul>
+
−
<p><li> <strong>Work Flow</strong>
+
−
</p>
+
−
</p><ul>
+
−
<li>
+
−
  Identify trigger sequence and target plasmid or probe
+
−
</p>
+
−
<li>
+
−
    Add target
+
−
</p>
+
−
<li>
+
−
    Incubation
+
−
</p>
+
−
<li>
+
−
    Evaluation
+
−
</p>
+
−
</p></ul>
+
  
−
    <p><li> <strong>Reagents</strong>
+
<!-- FORM TEMPLATE -->
−
</p>
+
<div class="preview-form">
−
</p><ul>
+
−
<li>
+
−
  Cell free expression kit
+
−
</p>
+
−
<li>
+
−
    •Promega L5540
+
−
</p>
+
−
<li>
+
−
    •Promega L1020
+
−
</p>
+
−
<li>
+
−
    •Promega L1110
+
−
</p>
+
−
<li>
+
−
    •NEB E6800L
+
−
</p>
+
−
<li>
+
−
  •Life Tech K990100
+
−
</p>
+
−
<li>
+
−
RNASE Inhibitor (RI)
+
−
</p>
+
−
<li>
+
−
Prepared tickets
+
−
</p>
+
−
<li>
+
−
Trigger Plasmid
+
−
<p/>
+
−
<li>
+
−
Substrate if needed
+
−
</p></ul>
+
−
   
+
−
</p>
+
  
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 
</div>
 
</div>
−
</tr>
+
<!-- LINKS TEMPLATE -->
−
<tr>
+
<div class="preview-item-links ">
 +
<div class="preview-links-wrapper">
  
−
<td colspan="12">
 
−
<h1 style="margin-top:15px; margin-bottom:25px; font-size:35px;text-align:center">qPCR- Quantitative Polymerase Chain Reaction Protocol</h1>
 
−
<div class = "well">
 
−
<p>
 
−
<ol type="I"><li><strong>Overview</strong><ul style="list-style-type:square">
 
−
</p>
 
−
<p>
 
−
    qPCR utilizes the same basis as standard polymerase chain reaction, in which a target section of DNA can be identified and amplified via an enzymatic reaction (DNA Polymerase) and two ssDNA oligos (primers). Where qPCR differs is in the addition of either dye or probe based chemistry (this protocol will be primarily deal with the former). What this dye allows for is detection of newly formed DNA through the incorporation of a small fluorescent molecule into the new double stranded DNA (dsDNA). When the relative fluorescent units (RFU) being monitored rises above the background level (established by experimental controls) it is possible to ascertain a concentration of target gene. The relationship being the more concentrated the target the earlier in the thermocycler program signal will be generated. </p></ul>
 
−
<p><li> <strong>Work Flow</strong>
 
−
</p>
 
−
</p><ul>
 
−
<li>
 
−
  Initial Prepartaion
 
−
</p>
 
−
<li>
 
−
    Create master mix
 
−
</p>
 
−
<li>
 
−
    Aliquot master mix
 
−
</p>
 
−
<li>
 
−
    Add target DNA and standards to appropriate wells
 
−
</p>
 
−
<li>
 
−
Start thermocycler
 
  
−
</p></ul>
 
  
−
    <p><li> <strong>Reagents</strong>
+
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
−
</p>
+
−
</p><ul>
+
</div>
−
<li>
+
</div>
−
  ICE
+
<!-- SOCIAL TEMPLATE -->
−
</p>
+
−
<li>
+
<div class="preview-social-wrapper removable-parent"  >
−
    Promega Hotstart Polyermase + Colorless Flexi buffer
+
<div id="vbid-c148c-utfyiy6p" class="preview-element preview-social-holder magic-circle-holder" data-menu-name="PREVIEW_SOCIAL" data-theme="8" >
−
</p>
+
−
<li>
+
<div id="FACEBOOK" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="FACEBOOK" data-img-url="//edit.www.imcreator.com/images/socialmedia/8facebook.png">
−
    10 mM dNTP
+
<a class='social-link-url' href="http://www.facebook.com/" target='_blank'><img class='preview-link-img ' src="//edit.www.imcreator.com/images/socialmedia/8facebook.png" ></a>
−
</p>
+
</div>
−
<li>
+
−
    25 mM MgCl
+
<div id="TWITTER" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="TWITTER" data-img-url="//edit.www.imcreator.com/images/socialmedia/8twitter.png">
−
</p>
+
<a class='social-link-url' href="http://www.twitter.com/" target='_blank'><img class='preview-link-img ' src="//edit.www.imcreator.com/images/socialmedia/8twitter.png" ></a>
−
<li>
+
</div>
−
    Molecular Water
+
−
</p>
+
<div id="INSTAGRAM" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="INSTAGRAM" data-img-url="//edit.www.imcreator.com/images/socialmedia/8instagram.png">
−
<li>
+
<a class='social-link-url' href="http://www.instagram.com" target='_blank'><img class='preview-link-img ' src="//edit.www.imcreator.com/images/socialmedia/8instagram.png" ></a>
−
  Forward and Reverse Primers in 10 uM
+
</div>
−
</p>
+
−
<li>
+
<div id="YOUTUBE" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="YOUTUBE" data-img-url="//edit.www.imcreator.com/images/socialmedia/8youtube.png">
−
  Evagreen dye 25x
+
<a class='social-link-url' href="http://www.youtube.com" target='_blank'><img class='preview-link-img ' src="//edit.www.imcreator.com/images/socialmedia/8youtube.png" ></a>
−
</p>
+
</div>
−
<li>
+
−
  Agarose
+
<div id="GOOGLE_PLUS" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="GOOGLE_PLUS" data-img-url="//edit.www.imcreator.com/images/socialmedia/8googleplus.png">
−
</p>
+
<a class='social-link-url' href="http://www.plus.google.com/" target='_blank'><img class='preview-link-img ' src="//edit.www.imcreator.com/images/socialmedia/8googleplus.png" ></a>
−
<li>
+
</div>
−
  Sybr Safe
+
 +
</div>  
 +
</div>
  
−
</p></ul>
+
<!-- RAW TEMPLATE -->
−
   
+
−
</p>
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
  
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="middle"></div>
 +
 +
</div>
 +
</div>
 +
<!-- FOOTERS END -->
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="light-box-wrapper  space-layer" style="display:none;">
 +
<div class="light-box-image-holder">
 +
<div class="light-box-image animated">
 +
 +
</div>
 +
<div class="lightbox-text-wrapper ">
 +
<div class="lightbox-text-holder animated">
 +
<div class="lightbox-title"></div>
 +
<div class="lightbox-subtitle"></div>
 +
<div id="paginator" style="display:none;">
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
    <img src="https://lh3.googleusercontent.com/EWqW7DEI4kOTRMLjK2-ObFHp-EYBt5apFYZ1LVFAhLtTLjigCRfx5hCCTKbIjIm68VQ00p9twloHJ9w8=s50" class="download-gallery-btn clickable" style="display: none;">
 +
<img src="https://lh3.googleusercontent.com/TgRyMQvJ3_h9RmOnu7AlhIE7NLOOBsRoBounARrs8fQv8HCRPaFtpBneSqJOSZpI6l7He_bAZKN179JBig=s50" class="close-lightbox-btn clickable" style="opacity: 1;">
 +
<img src="https://lh3.googleusercontent.com/43-pXHjwrpmVO8Oean-6BD0uzARvcqUQrpdi7Yw2bxaXwEoP21UdN5kW6Ks9pdOxf7ropMUrh0djgYPwYPU=s50" class="lightbox-arrow lightbox-left clickable top-layer" >
 +
<img src="https://lh3.googleusercontent.com/9rwgVnDglPdPFugSu98fhDmxzjXC9KovZ_7BuHkXPIv6jvg9S96flGnhL_e4y8mIpPpZQstfqEV-WitY=s50" class="lightbox-arrow lightbox-right clickable top-layer" >
 
</div>
 
</div>
−
</tr>
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
<td colspan="12">
 
−
<h1 style="margin-top:15px; margin-bottom:25px; font-size:35px;text-align:center">Experimental Outline
 
−
<div class = "well">
 
−
<p>
 
−
<ol type="I"><li><strong>Pre-Phase One Experiments</strong><ul style="list-style-type:square">
 
−
</p>
 
−
<p>
 
−
  Establish control experiments
 
−
</p>
 
−
<li>
 
−
-Constitutive GFP
 
−
<p/>
 
−
<li>
 
−
o Switch H GFP
 
−
<p/>
 
−
</ul>
 
−
<p><li> <strong>Pre-Phase One Goals</strong>
 
−
</p>
 
−
</p><ul>
 
−
<li>
 
−
  The goal of pre-phase 1 is to establish a working positive control and negative control in lab.
 
−
</p></ul>
 
−
 
−
 
−
    <p><li> <strong>Phase One Experiments</strong>
 
−
 
−
</p><ul>
 
−
<li>
 
−
  Clone composite circuit in e.coli
 
−
</p>
 
−
<li>
 
−
    Test efficacy of the circuit in vivo and determine threshold limits of both arsenic and arsenite
 
−
</p>
 
−
<li>
 
−
    Perform mini prep and quantify
 
−
</p></ul>
 
−
   
 
−
 
−
 
−
<p><li> <strong>Phase One Goals</strong>
 
−
 
−
</p><ul>
 
−
<li>
 
−
  The goals of this phase is to grow a large amount of the composite circuit and develop an understanding of whether or not the circuit is biologically effective. Another primary goal is to develop an understanding of how the circuit works in its entirety (upper threshold/lower threshold)
 
−
</p></ul>
 
−
 
−
 
−
<p><li> <strong>Phase Two Experiments</strong>
 
−
 
−
</p><ul>
 
−
<li>
 
−
  Test the efficacy of the composite circuit instead of crude lysate
 
−
</p>
 
−
<li>
 
−
    Test purified plasmid with Promega S30 kit
 
−
</p></ul>
 
−
 
−
 
−
 
−
<p><li> <strong>Phase Two Goals</strong>
 
−
 
−
</p><ul>
 
−
<li>
 
−
  The goal of this phase is to take the circuit out of the cell and test it in situ utilizing both a bacterial expression kit (Promega S30) and an attempt at functionalizing the circuit utilizing a formulated crude lysate for increased application space.
 
−
</p></ul>
 
−
 
−
 
−
<p><li> <strong>Phase Three Experiments</strong>
 
−
 
−
</p><ul>
 
−
<li> Attempt at micro scaling reaction
 
−
<p/>
 
−
<li>
 
−
Porting reactions to paper based system
 
−
<p/>
 
−
<li>
 
−
Attempt at lyophilizing the circuit on paper both with Promega S30 kit and in the crude lysate form (if the previous phase demonstrated reasonable evidence for progressing on that front)
 
−
<p/><ul>
 
−
 
−
 
−
<p><li> <strong>Phase Three Goals</strong>
 
−
 
−
</p><ul>
 
−
<li>
 
−
  The goal of this phase is to successfully micro scale the reaction and port it to a lateral flow paper based system. If successful in this phase, we will have developed a biological means to detect presence of arsenic and arsenite utilizing a lateral flow paper system that is both cost effective and easy to implement.
 
−
</p></ul>
 
−
 
  
 +
</div>
 +
 +
<script src="//edit.www.imcreator.com/js/lightbox.js?v=1.4.0p1"  type="text/javascript"></script>
 +
<script src="//edit.www.imcreator.com/js/spimeengine.js?v=1.4.0p1"  type="text/javascript"></script>
 +
 +
 +
<!--  SHOPROCKET  ECOMMERCE  -->
 +
<script>
 +
var JSR = jQuery.noConflict();
 +
var $ = jQuery.noConflict();
 +
</script>
 +
 +
<input type="hidden" name="sr-companyid" id="sr-companyid" value="">
 +
</body>
  
 +
 +
<div id="user-pref" style="display:none;" data-lang="en"></div>
 +
<div style="display:none;" id="lightbox-menus-holder">
 +
 +
<div  class="login-dialog-wrapper white_content delay-anim" style="text-align:center;">
  
 +
<div class="login dialog-holder">
 +
<div  class="login-title"><span class="im-text form-type  t-t">Login</span></div>
 +
<form id="login-form">
 +
<img id="user-gravatar" src="https://www.gravatar.com/avatar/?d=mm" /><br/>
 +
<input name="name" type="text" placeholder="nickname/email" data-placeholder="nickname/email" /><br/>
 +
<input name="password" type="password" placeholder="password" data-placeholder="password" autocomplete="off" /><br/>
 +
<input id="login-btn" class="clickable" type="button" class="input-btn" value="LOGIN">
 +
<div id="goto-register" class="clickable t-t">Create a new account</div>
 +
<div id="forgot-password" class="clickable t-t">Forgot Password?</div>
 +
</form>
 +
</div>
 +
 +
<div class="register dialog-holder" style="display:none;">
 +
<div  class="login-title"><span class="im-text form-type  t-t">Login</span></div>
 +
<form id="register-form" class="hide-phone">
 +
<img id="user-gravatar" src="https://www.gravatar.com/avatar/?d=mm" /><br/>
 +
<input name="email" type="text" placeholder="email" data-placeholder="email"  /><br/>
 +
 +
<input name="name" type="text" placeholder="nickname" data-placeholder="nickname" /><br/>
 +
<input name="password" type="password"  placeholder="password" data-placeholder="password" autocomplete="off" /><br/>
 +
 +
<input id="terms" type="checkbox" checked="checked" disabled /><span class="form-label"><a href="http://www.imcreator.com/terms-of-service" target="_blank" class="terms-link  t-t">Terms and Conditions</a></span><br/>
 +
<input id="register-btn" class="clickable" type="button" class="input-btn" value="JOIN">
 +
<div id="goto-login" class="clickable t-t">Already a member?</div>
 +
</form>
 +
</div>
 +
 +
<div class="forgot-pass dialog-holder" style="display:none;">
 +
<div  class="login-title"><span class="im-text form-type t-t">Login</span></div>
 +
<form id="forgot-pass-form">
 +
<img id="user-gravatar" src="https://www.gravatar.com/avatar/?d=mm" /><br/>
 +
<input name="email" type="text" placeholder="email" data-placeholder="email" /><br/>
 +
<input id="forgot-pass-btn" class="clickable" type="button" class="input-btn" value="SEND">
 +
<div id="goto-login" class="clickable t-t">Back</div>
 +
</form>
 +
</div>
 
</div>
 
</div>
−
</tr>
+
</div>
−
 
+
<div id="fade" class="black_overlay fadein-noscript" style="display:none;"></div>
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
  
  
  
 
</html>
 
</html>

Revision as of 22:39, 19 June 2017

HOME
TEAM
PROJECT
Description
Design
Experiments
Notebook
InterLab
Contribution
Model
Results
Demonstrate
Improve
Attributions
PARTS
Parts
Basic Parts
Composite Parts
Part Collection
SAFETY
HUMAN PRACTICES
Silver HP
Integrated and Gold
Public Engagement
AWARDS
Applied Design
Entrepreneurship
Hardware
Measurement
Model
Plant
Software
JUDGING FORM

Cadets2Vets

EXPERIMENTS - CADETS2VETS

Menu



EXPERIMENTS.



RESEARCH. PROTOCOLS.



TICKETED CELL FREE EXPRESSION SYSTEM PROTOCOLS



I. Overview

The idea of cell free expression has existed for more than 20 years. It is the notion of taking the elements required for both transcription and translation and utilizing them for an external protein expression system. This is extremely beneficial for expression of cytotoxic proteins in which high yields are difficult to produce in vivo. For our purpose within this assay, this concept is utilized in conjunction with a regulatory factor for detection. This is detection is predicated either by regulation of the RBS at the transcript level or by sequestering the binding sight of RNA polymerase.

II. Work Flow

§ Identify trigger sequence and target plasmid or probe

§ Add target

§ Incubation

§ Evaluation

III. Reagents

§ Cell free expression kit

§ •Promega L5540

§ •Promega L1020

§ •Promega L1110

§ •NEB E6800L

§ •Life Tech K990100

§ RNASE Inhibitor (RI)

§ Prepared tickets

§ Trigger Plasmid

§ Substrate if needed



aPCR- Quantitative Polymerase Chain Reaction Protocol





Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed malesuada faucibus ex nec ultricies. Donec mattis egestas nisi non pretium. Suspendisse nec eros ut erat facilisis maximus. In congue et leo in varius. Vestibulum sit amet felis ornare, commodo orci ut, feugiat lorem.


Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed malesuada faucibus ex nec ultricies. Donec mattis egestas nisi non pretium. Suspendisse nec eros ut erat facilisis maximus. In congue et leo in varius. Vestibulum sit amet felis ornare, commodo orci ut, feugiat lorem.


Experimental outline





I. Pre-Phase One Experiments

Establish control experiments

§ -Constitutive GFP

§ o Switch H GFP

II. Pre-Phase One Goals

§ The goal of pre-phase 1 is to establish a working positive control and negative control in lab.

III. Phase One Experiments

§ Clone composite circuit in e.coli

§ Test efficacy of the circuit in vivo and determine threshold limits of both arsenic and arsenite

§ Perform mini prep and quantify

IV. Phase One Goals

§ The goals of this phase is to grow a large amount of the composite circuit and develop an understanding of whether or not the circuit is biologically effective. Another primary goal is to develop an understanding of how the circuit works in its entirety (upper threshold/lower threshold)

V. Phase Two Experiments

§ Test the efficacy of the composite circuit instead of crude lysate

§ Test purified plasmid with Promega S30 kit

VI. Phase Two Goals

§ The goal of this phase is to take the circuit out of the cell and test it in situ utilizing both a bacterial expression kit (Promega S30) and an attempt at functionalizing the circuit utilizing a formulated crude lysate for increased application space.

VII. Phase Three Experiments

§ Attempt at micro scaling reaction

§ Porting reactions to paper based system

§ Attempt at lyophilizing the circuit on paper both with Promega S30 kit and in the crude lysate form (if the previous phase demonstrated reasonable evidence for progressing on that front)

§ Phase Three Goals

§ The goal of this phase is to successfully micro scale the reaction and port it to a lateral flow paper based system. If successful in this phase, we will have developed a biological means to detect presence of arsenic and arsenite utilizing a lateral flow paper system that is both cost effective and easy to implement.


Follow us on Social Media!


2304 Jefferson Ave, Tacoma, WA 98402