Line 197: | Line 197: | ||
<tr> | <tr> | ||
<td><p><font size="-1">c)</font></p><img src="https://static.igem.org/mediawiki/2017/b/bc/T-Nanjing-China-h2s-9.png" width="400" /><p><font size="-1"></p></p></td> | <td><p><font size="-1">c)</font></p><img src="https://static.igem.org/mediawiki/2017/b/bc/T-Nanjing-China-h2s-9.png" width="400" /><p><font size="-1"></p></p></td> | ||
− | <td><p><font size="-1">a)RFP responsiveness of the detector system.<br /> | + | <td><p><font size="-1">Figure 2.a)RFP responsiveness of the detector system.<br /> |
b) A visible photograph of a).<br /> | b) A visible photograph of a).<br /> | ||
c) Test of selectivity.</font></p></td> | c) Test of selectivity.</font></p></td> |
Revision as of 04:22, 28 October 2017
Results
In the part of lab work, we have designed three biosensor sequence and improved an old part, J23000. What's more, all the three design have been demonstrate by us.
|
|
|
|
Figure 3. Influence of Formaldehyde Induce Time on Fluorescence Expression |
Figure4.A photograph of E.coli cells containing the formaldehyde-induced RFP expression plasmid, or without formaldehyde induction, and re-suspended in PBS buffer(pH7.4) |
|
|
It is worth to be mentioned that the team OUC help us demonstrate the result. |
|
|
In the experiment, we proved that the sequence worked well and was useful to detect hydrogen sulfide | |
Figure1.Whole-cell sequence dual-enzyme digestion |
|
a) |
b) |
c) |
Figure 2.a)RFP responsiveness of the detector system. |
There is a composite of hydrogen sensor full length sequence. The order of the elements is: HoxA-HoxB-HoxC-HoxJ-terminator-HoxP-EGFP. The sequence of HoxABCJP comes from Ralstonia eutropha H16 megaplasmid pHG1. The hole sequence acts as an hydrogen sensor. When the amount of hydrogen goes to a higher level, Fluorescence intensity increases apparently. | |
The sequence was a good detecter in the lab work. | |
Figure1. Coomassie Brilliant Blue R-250-stained SDS-Page analysis of recombinant E.coli expressing hoxABCJ-terminator-hoxp-gfp |
Fingure 2. Western blot analysis of recombinant E.coli expressing his-hoxA |
|
|
Figure 3. Influence of H2 concentration on fluorescence expression |