Difference between revisions of "Team:WHU-China/Composite Part"

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<h3>★  ALERT! </h3>
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  <img id="background-image" src="https://static.igem.org/mediawiki/2017/6/69/WHU-China-child-bg.jpeg"  alt=""/>
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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          <ul class="nv">
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            <li id="nv0" style="width:200px"><a href="https://2017.igem.org/Team:WHU-China"></a></li>
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            <li id="nv1">
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              <a>Project</a>
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              <ul>
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                <li id="nv11"><a href="https://2017.igem.org/Team:WHU-China/Overview">Overview</a></li>
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                <li id="nv12"><a href="https://2017.igem.org/Team:WHU-China/Background">Background</a></li>
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                <li id="nv13"><a href="https://2017.igem.org/Team:WHU-China/Introduction">Introduction</a></li>
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                <li id="nv14"><a href="https://2017.igem.org/Team:WHU-China/Designs">Designs</a></li>
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                <li id="nv15"><a href="https://2017.igem.org/Team:WHU-China/Experiments">Experiments</a></li>
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                <li id="nv16"><a href="https://2017.igem.org/Team:WHU-China/Results">Results</a></li>
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              </ul>
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            </li>
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            <li id="nv2"><a>Parts</a>
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            <ul>
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                <li id="nv21"><a href="https://2017.igem.org/Team:WHU-China/Description_Part">Description</a></li>
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                <li id="nv22"><a href="https://2017.igem.org/Team:WHU-China/Basic_Part">Basic</a></li>
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                <li id="nv23"> <a href="https://2017.igem.org/Team:WHU-China/Composite_Part">Composite</a></li>
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</ul>
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            </li>
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            <li id="nv3"><a>Interlab</a>
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                <ul>
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                <li id="nv31"><a href="https://2017.igem.org/Team:WHU-China/Interlab">Results</a></li>
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                <li id="nv32"><a href="https://2017.igem.org/Team:WHU-China/Calibration">Calibration</a></li>
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                <li id="nv33"><a href="https://2017.igem.org/Team:WHU-China/Cell_Measuremnet">CellMeasuremnet</a></li>
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</ul>
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            </li>
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            <li id="nv4"><a href="https://2017.igem.org/Team:WHU-China/Notebook">Notebook</a>
  
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              </li>
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            <li id="nv5">
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            <a>Model</a>
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            <ul>
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            <li id="nv51"><a href="https://2017.igem.org/Team:WHU-China/Model">Modeling</a></li>
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                <li id="nv52"><a href="https://2017.igem.org/Team:WHU-China/Hardware">Hardware</a></li>
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            </ul>
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            </li>
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            <li id="nv6">
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            <a>Human Practice</a>
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              <ul>
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                <li id="nv61"><a href="https://2017.igem.org/Team:WHU-China/HP/Silver">Silver</a></li>
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                <li id="nv62"><a href="https://2017.igem.org/Team:WHU-China/HP/Gold_Integrated">Gold&Integrated</a></li>
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                <li id="nv63"><a href="https://2017.igem.org/Team:WHU-China/Engagement">Engagement</a></li>
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              </ul>
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            </li>
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            <li id="nv7"><a>Safety</a>
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            <ul>
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                <li id="nv71"><a href="https://2017.igem.org/Team:WHU-China/Project_Design">ProjectDesign</a></li>
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                <li id="nv72"><a href="https://2017.igem.org/Team:WHU-China/Lab_Work">LabWork</a></li>
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              </ul>
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            </li>
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            <li id="nv8"><a>Team</a>
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            <ul>
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                <li id="nv81"><a href="https://2017.igem.org/Team:WHU-China/Member">Member</a></li>
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                <li id="nv82"><a href="https://2017.igem.org/Team:WHU-China/Attributions">Attributions</a> </li>
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                <li id="nv83"><a href="https://2017.igem.org/Team:WHU-China/Collaborations">Collaborations</a></li>
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              </ul>
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            </li>
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            <li id="nv9"><a href="https://2017.igem.org/Team:WHU-China/Achievement">Achievement</a>
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              </li>
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          </ul>
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        </div>
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      </div>
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        <h1>Composite Parts</h1>
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        <ul id="cont">
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  <h2>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Brick2 RdhANP combined with a biosensor (BBa_K2462007)</h2>
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<h3>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;As we designed our project, we have wondered if there exists any regulation mechanism to make the expression of RdhANP more precisely. We searched the information and find a biosensor created by team Peking-China in 2013. This biosensor can function when it runs into substances analogous to salicylic acid. And some of the substances they tested can serve as the substrates of RdhANP , like 2,4,6-trichloro-phenol. We then linked this sensor(BBa_J61051) with RdhANP and the enzyme is supposed to express when it's substrates are available.
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The biosensor made by Peking-China: http://parts.igem.org/Part:BBa_J61051
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<p align="center"> <img src="https://static.igem.org/mediawiki/2017/2/2b/WHU-China-Composite.png" width="800" height="300" alt=""/>
  
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<h2>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Brick7 Combination of promoters in brick4 (BBa_K2462004) with RBS(BBa_B0030) (BBa_K2462006)</h2>
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<h3>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We improve a previous part(BBa_B0030) by combining it with the promoter used in Bacillus megaterium. As the strength of RBS(BBa_B0030) has already been tested and the promoters of these two composite bricks are suitable for Bacillus megaterium, it’s quite convenient for other teams to use.
  
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<h1>Composite Parts</h1>
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<p>
 
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
 
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<h3>Best Composite Part Special Prize</h3>
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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<h4>Note</h4>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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Revision as of 06:53, 28 October 2017

Composite Parts

         Brick2 RdhANP combined with a biosensor (BBa_K2462007)

         As we designed our project, we have wondered if there exists any regulation mechanism to make the expression of RdhANP more precisely. We searched the information and find a biosensor created by team Peking-China in 2013. This biosensor can function when it runs into substances analogous to salicylic acid. And some of the substances they tested can serve as the substrates of RdhANP , like 2,4,6-trichloro-phenol. We then linked this sensor(BBa_J61051) with RdhANP and the enzyme is supposed to express when it's substrates are available. The biosensor made by Peking-China: http://parts.igem.org/Part:BBa_J61051

         Brick7 Combination of promoters in brick4 (BBa_K2462004) with RBS(BBa_B0030) (BBa_K2462006)

         We improve a previous part(BBa_B0030) by combining it with the promoter used in Bacillus megaterium. As the strength of RBS(BBa_B0030) has already been tested and the promoters of these two composite bricks are suitable for Bacillus megaterium, it’s quite convenient for other teams to use.