Difference between revisions of "Team:KUAS Korea/Design"

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<h4> Detection System 1: hssR/S Mechanism </h4><br>
 
<h4> Detection System 1: hssR/S Mechanism </h4><br>
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                        <img src="https://static.igem.org/mediawiki/2017/3/35/T--KUAS_Korea--HssRS.png">
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<p><font size=4>The hssR and hssS mechanism of our project is as follows.</font></p>
 
<p><font size=4>The hssR and hssS mechanism of our project is as follows.</font></p>
 
<p><font size=4>      ① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br>
 
<p><font size=4>      ① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br>

Revision as of 07:27, 28 October 2017

Design

POO-robiotics




Detection System 1: hssR/S Mechanism



The hssR and hssS mechanism of our project is as follows.

① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.
③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.
④ The phosphorylated HssR binds to the direct repeat sequence of the hrtAB promoter and initiates the reporter’s transcription.


Detection System 2: hrtR Mechanism





Also, the mechanism of heme detection using hrtR protein is as follows.

①If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.
③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.