Difference between revisions of "Team:Munich/Protocols"

Revision as of 14:11, 28 October 2017

Protocols
For our project we used different methods which are described in respective protocols. We also included a detailed list of all chemicals, materials and the aim of each experiment. This page is for everybody who wants to reproduce our work or wants to get an overview, but also to share our experiences with the iGEM community.
General Cas13a Read-out Other Agarose gel electrophoresis PCR Minipreparation Restriction digest Ligation Glycerol stock Ni-NTA agarose protein purification Äkta protein purification SDS PAGE RNase activity Lyophilization Expression of Cas13a and TEV Protease Phenol/Chlorophorm purification of RNA Phenol/Chlorophorm purification of genomic DNA Denaturing Urea PAGE In vitro Transcription RNA purification with Norgen Kit FINA method for RNA extraction FINA method for DNA extraction First strand cDNA syntesis RPA RPA on paper RPA TX Chemical transformation Electro transformation Chemically competent cells Electro competent cells Dephosphorylation Golden Gate Assembly Gibson Assembly PCR clean-up Sequencing sample preparation Soft litography AuNP preparation AuNP-DNA conjugation AuNP linkage AuNP cleavage
Protein and nucleic acid sequences
Proteins Nucleic acids

@@ Line 134: / Line 134: @@
      	<div class="myProtocol general">
 			<input type="checkbox" id="miniprep ">
-			<h2><label for="miniprep ">Miniprep </label></h2>
+			<h2><label for="miniprep ">Minipreparation</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--miniprep.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/2/27/T--Munich--protocol--miniprep.pdf" > Click here! </a> </p>
@@ Line 169: / Line 169: @@
     <div class="myProtocol cas13a">
 			<input type="checkbox" id="protpurNiNTA">
-			<h2><label for="protpurNiNTA">Protein purification with Ni-NTA</label></h2>
+			<h2><label for="protpurNiNTA">Ni-NTA agarose protein purification</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/b/bc/T--Munich--protocol--protein_purification_Ni_NTA.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/b/bc/T--Munich--protocol--protein_purification_Ni_NTA.pdf" > Click here! </a> </p>
@@ Line 177: / Line 177: @@
     <div class="myProtocol cas13a">
 			<input type="checkbox" id="protpurAkta">
-			<h2><label for="protpurAkta">Protein purification with Äkta</label></h2>
+			<h2><label for="protpurAkta">Äkta protein purification</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--cas13a_purif_akta.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/2/20/T--Munich--protocol--cas13a_purif_akta.pdf" > Click here! </a> </p>
@@ Line 185: / Line 185: @@
     <div class="myProtocol general">
 			<input type="checkbox" id="SDSpage">
-			<h2><label for="SDSpage">SDS page </label></h2>
+			<h2><label for="SDSpage">SDS PAGE</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/2/21/T--Munich--protocol--sds_page.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/2/21/T--Munich--protocol--sds_page.pdf" > Click here! </a> </p>
@@ Line 195: / Line 195: @@
     <div class="myProtocol readout">
 			<input type="checkbox" id="RNAseActi">
-			<h2><label for="RNAseActi">RNAse activity protocol</label></h2>
+			<h2><label for="RNAseActi">RNase activity</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/4/40/T--Munich--protocol--rnase_activity.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/4/40/T--Munich--protocol--rnase_activity.pdf" > Click here! </a> </p>
@@ Line 211: / Line 211: @@
     <div class="myProtocol general">
 			<input type="checkbox" id="expression">
-			<h2><label for="expression">Protein expression</label></h2>
+			<h2><label for="expression">Expression of Cas13a and TEV Protease</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/1/19/T--Munich--protocol--protein_expression.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/1/19/T--Munich--protocol--protein_expression.pdf" > Click here! </a> </p>
@@ Line 220: / Line 220: @@
 	   <div class="myProtocol other">
 			<input type="checkbox" id="pheChlorRNA">
-			<h2><label for="pheChlorRNA">Phenol-chlorophorm purification of RNA</label></h2>
+			<h2><label for="pheChlorRNA">Phenol/Chlorophorm purification of RNA</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/0/0b/T--Munich--protocol--phenol_chlorophorm_purification_rna.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/0/0b/T--Munich--protocol--phenol_chlorophorm_purification_rna.pdf" > Click here! </a> </p>
@@ Line 228: / Line 228: @@
 			<div class="myProtocol other">
 			<input type="checkbox" id="PheChloroDNA">
-			<h2><label for="PheChloroDNA">Phenol-chlorophorm purification of genomic DNA</label></h2>
+			<h2><label for="PheChloroDNA">Phenol/Chlorophorm purification of genomic DNA</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/2/2b/T--Munich--protocol--phenol_chlorophorm_purification_dna.pdf" width="100%" height="100%">
 					<p>  <a href="https://static.igem.org/mediawiki/2017/2/2b/T--Munich--protocol--phenol_chlorophorm_purification_dna.pdf" > Click here! </a> </p>
@@ Line 235: / Line 235: @@
 	   <div class="myProtocol general">
 			<input type="checkbox" id="UreaRNA">
-			<h2><label for="UreaRNA">Urea gel for RNA detection </label></h2>
+			<h2><label for="UreaRNA">Denaturing Urea PAGE</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/c/cc/T--Munich--protocol--urea_page.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/c/cc/T--Munich--protocol--urea_page.pdf" > Click here! </a> </p>
@@ Line 243: / Line 243: @@
 		   <div class="myProtocol general">
 			<input type="checkbox" id="vitrotranscription">
-			<h2><label for="vitrotranscription">In vitro transcription</label></h2>
+			<h2><label for="vitrotranscription"><i>In vitro</i> Transcription</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/0/08/T--Munich--protocol--invitro_transcription.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/0/08/T--Munich--protocol--invitro_transcription.pdf" > Click here! </a> </p>
@@ Line 277: / Line 277: @@
 	   <div class="myProtocol other">
 			<input type="checkbox" id="cDNA">
-			<h2><label for="cDNA">First strand cDNA syntesis (NEB)</label></h2>
+			<h2><label for="cDNA">First strand cDNA syntesis</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--first_strand_cdna_synthesis.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/7/71/T--Munich--protocol--first_strand_cdna_synthesis.pdf" > Click here! </a> </p>
@@ Line 320: / Line 320: @@
 	   <div class="myProtocol general">
 			<input type="checkbox" id="Electrotrafo">
-			<h2><label for="Electrotrafo">Electro-transformation</label></h2>
+			<h2><label for="Electrotrafo">Electro transformation</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/7/7e/T--Munich--protocol--e_trafo.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/7/7e/T--Munich--protocol--e_trafo.pdf" > Click here! </a> </p>
@@ Line 336: / Line 336: @@
 		   <div class="myProtocol general">
 			<input type="checkbox" id="ecells">
-			<h2><label for="ecells">Electro-competent cells</label></h2>
+			<h2><label for="ecells">Electro competent cells</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--e_comp_cells.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/f/ff/T--Munich--protocol--e_comp_cells.pdf" > Click here! </a> </p>
@@ Line 352: / Line 352: @@
 		   <div class="myProtocol general">
 			<input type="checkbox" id="GoldenGate">
-			<h2><label for="GoldenGate">Golden Gate</label></h2>
+			<h2><label for="GoldenGate">Golden Gate Assembly</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/9/9c/T--Munich--protocol--golden_gate.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/9/9c/T--Munich--protocol--golden_gate.pdf" > Click here! </a> </p>
@@ Line 361: / Line 361: @@
 			   <div class="myProtocol general">
 			<input type="checkbox" id="gibson">
-			<h2><label for="gibson">Gibson</label></h2>
+			<h2><label for="gibson">Gibson Assembly</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/f/fa/T--Munich--protocol--gibson.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/f/fa/T--Munich--protocol--gibson.pdf" > Click here! </a> </p>
@@ Line 368: / Line 368: @@
 			   <div class="myProtocol general">
 			<input type="checkbox" id="PCRcleanup">
-			<h2><label for="PCRcleanup">PCR cleanup</label></h2>
+			<h2><label for="PCRcleanup">PCR clean-up</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/0/05/T--Munich--protocol--pcr_clean_up.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/0/05/T--Munich--protocol--pcr_clean_up.pdf" > Click here! </a> </p>
@@ Line 377: / Line 377: @@
 			   <div class="myProtocol general">
 			<input type="checkbox" id="seqprep">
-			<h2><label for="seqprep">Sequencing sample prep</label></h2>
+			<h2><label for="seqprep">Sequencing sample preparation</label></h2>
 			<object data="https://static.igem.org/mediawiki/2017/5/5b/T--Munich--protocol--sequencing_prep.pdf" width="100%" height="100%">
 			<p>  <a href="https://static.igem.org/mediawiki/2017/5/5b/T--Munich--protocol--sequencing_prep.pdf" > Click here! </a> </p>

Difference between revisions of "Team:Munich/Protocols"

Revision as of 14:11, 28 October 2017

Agarose gel electrophoresis

PCR

Minipreparation

Restriction digest

Ligation

Glycerol stock

Ni-NTA agarose protein purification

Äkta protein purification

SDS PAGE

RNase activity

Lyophilization

Expression of Cas13a and TEV Protease

Phenol/Chlorophorm purification of RNA

Phenol/Chlorophorm purification of genomic DNA

Denaturing Urea PAGE

In vitro Transcription

RNA purification with Norgen Kit

FINA method for RNA extraction

FINA method for DNA extraction

First strand cDNA syntesis

RPA

RPA on paper

RPA TX

Chemical transformation

Electro transformation

Chemically competent cells

Electro competent cells

Dephosphorylation

Golden Gate Assembly

Gibson Assembly

PCR clean-up

Sequencing sample preparation

Soft litography

AuNP preparation

AuNP-DNA conjugation

AuNP linkage

AuNP cleavage