Line 182: | Line 182: | ||
<br> • <i>E. Coli</i> DH10B cells made chemically competent </br> | <br> • <i>E. Coli</i> DH10B cells made chemically competent </br> | ||
<br> • Golden Gate Assembly of BB1_01, BB1_02, BB1_03, BB1_04, BB1_05, BB1_06, BB1_07, BB1_08 </br> | <br> • Golden Gate Assembly of BB1_01, BB1_02, BB1_03, BB1_04, BB1_05, BB1_06, BB1_07, BB1_08 </br> | ||
− | </p> | + | </p></div> |
− | + | ||
<a href="#adrfou" id="Week 2" class="arrows" onclick="expand_collapse('2');"><pre><font size="+2"><b>Week 2</b></font> (10/07-14/07)</pre></a><span style="font-size:150%;"><b> </b></span> | <a href="#adrfou" id="Week 2" class="arrows" onclick="expand_collapse('2');"><pre><font size="+2"><b>Week 2</b></font> (10/07-14/07)</pre></a><span style="font-size:150%;"><b> </b></span> | ||
− | <div id="2" class="yolo" style="display:none;"><p> | + | <div id="2" class="yolo" style="display:none;"> |
+ | <p> | ||
+ | <br>• PCR: 1, 2, 3, 4, 6, 15, 18, 20, 23, 24, 25, 26</br> | ||
+ | Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification according to <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol">protocol</a>. | ||
+ | |||
+ | </p></div> | ||
<a href="#nxbfgr" id="Week 3" class="arrows" onclick="expand_collapse('3');"><pre><font size="+2"><b>Week 3</b></font> (17/07-21/07)</pre></a><span style="font-size:150%;"><b> </b></span> | <a href="#nxbfgr" id="Week 3" class="arrows" onclick="expand_collapse('3');"><pre><font size="+2"><b>Week 3</b></font> (17/07-21/07)</pre></a><span style="font-size:150%;"><b> </b></span> |
Revision as of 19:16, 28 October 2017
Notebook.
All protocols we used for the methods can be found here:
Protocol
Week 1 (03/07-07/07)
Week 2 (10/07-14/07)
Week 3 (17/07-21/07)
Week 4 (24/07-28/07)
Week 5 (31/07-04/08)
Week 6 (07/08-11/08)
Week 7 (14/08-18/08)
Week 8 (21/08-25/08)
Week 9 (28/08-01/09)
Week 10 (04/09-08/09)
Week 11 (11/09-15/09)
Week 12 (18/09-22/09)
Week 13 (25/09-29/09)