Difference between revisions of "Team:Cadets2Vets/Demonstrate"

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<head>
 
<head>
 
<title>
 
<title>
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Demonstrate - CADETS2VETS</title>
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Demonstrate - USE THIS ONE!!!</title>
 
<!-- META -->
 
<!-- META -->
 
<meta charset="UTF-8">
 
<meta charset="UTF-8">
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<link rel="stylesheet" type="text/css" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css" />
 
<link rel="stylesheet" type="text/css" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css" />
 
 
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<link id="style-c148c-oayvzg7x" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-c148c-oayvzg7x&viewer=xprs&parent_style=style-f6516e81-dzebo07f&v=1.4.0p1">
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<link id="style-59a5b-weduyzcw" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-weduyzcw&viewer=xprs&parent_style=style-59a5b-weduyzcw&v=1.4.0p1">
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<link id="style-f6516e81-dzebo07f" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-f6516e81-dzebo07f&viewer=xprs&parent_style=style-f6516e81-dzebo07f&v=1.4.0p1">
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<link id="style-59a5b-oolaaxjn" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-oolaaxjn&viewer=xprs&parent_style=style-59a5b-weduyzcw&v=1.4.0p1">
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<link id="style-559f00b3-pp37vzbi" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-559f00b3-pp37vzbi&viewer=xprs&parent_style=style-f6516e81-dzebo07f&v=1.4.0p1">
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<link id="style-59a5b-lbaqmrpi" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-lbaqmrpi&viewer=xprs&parent_style=style-59a5b-weduyzcw&v=1.4.0p1">
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<link id="style-c148c-zjadrzwq" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-c148c-zjadrzwq&viewer=xprs&parent_style=style-f6516e81-dzebo07f&v=1.4.0p1">
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<link id="style-59a5b-jbqq3wgo" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-jbqq3wgo&viewer=xprs&parent_style=style-59a5b-weduyzcw&v=1.4.0p1">
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<link id="style-c148c-ilf5hgbo" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-c148c-ilf5hgbo&viewer=xprs&parent_style=style-f6516e81-dzebo07f&v=1.4.0p1">
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<link id="style-59a5b-yamofliu" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-yamofliu&viewer=xprs&parent_style=style-59a5b-weduyzcw&v=1.4.0p1">
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<link id="style-8d2b75a7-ahof2xa9" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-8d2b75a7-ahof2xa9&viewer=xprs&parent_style=style-f6516e81-dzebo07f&v=1.4.0p1">
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<link id="style-59a5b-erfb0r5q" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-erfb0r5q&viewer=xprs&parent_style=style-59a5b-weduyzcw&v=1.4.0p1">
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<link id="vbid-f6516e81-lok1anrm-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_data_css?page_id=vbid-f6516e81-lok1anrm-STRIPE_DATA&v=1.4.0p1">  
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<link id="vbid-59a5b-xghz2dmu-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_data_css?page_id=vbid-59a5b-xghz2dmu-STRIPE_DATA&v=1.4.0p1">  
 
 
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<link id="vbid-c148c-nxe9zty7-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_data_css?page_id=vbid-c148c-nxe9zty7-STRIPE_DATA&v=1.4.0p1">  
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<link id="vbid-59a5b-fnxcttvm-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_data_css?page_id=vbid-59a5b-fnxcttvm-STRIPE_DATA&v=1.4.0p1">  
 
 
 
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/fonts.css?v=1.4.0p1" />
 
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/fonts.css?v=1.4.0p1" />
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</script></head>
 
</script></head>
  
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<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-c148c-nxe9zty7" data-root-style-id="style-c148c-ilf5hgbo" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.0p1" data-caller="preview"  data-ecommerce-dashboard="http://dashboard.shoprocket.co" data-static-server="//edit.www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
+
<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-59a5b-fnxcttvm" data-root-style-id="style-59a5b-yamofliu" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.0p1" data-caller="preview"  data-ecommerce-dashboard="http://dashboard.shoprocket.co" data-static-server="//edit.www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
 
 
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<div id="xprs" data-website-name="CADETS2VETS" class="xprs-holder" >
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<div id="xprs" data-website-name="USE THIS ONE!!!" class="xprs-holder" >
 
<div class="main-page "  >
 
<div class="main-page "  >
 
<div id="content">
 
<div id="content">
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<div  id="vbid-f6516e81-lok1anrm"  class="master container style-f6516e81-dzebo07f content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Demonstrate"  data-itemslug="demonstrate" data-itemstyleid="style-f6516e81-dzebo07f" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-f6516e81-lok1anrm" data-preset-type-id="ITEMS" data-preview-style="style-f6516e81-dzebo07f" data-style="style-5a24e-kzdyurm3ju" data-absolute-path="http://edit.www.imcreator.com/viewer" >
+
<div  id="vbid-59a5b-xghz2dmu"  class="master container style-59a5b-weduyzcw content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Demonstrate"  data-itemslug="demonstrate" data-itemstyleid="style-59a5b-weduyzcw" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-59a5b-xghz2dmu" data-preset-type-id="ITEMS" data-preview-style="style-59a5b-weduyzcw" data-style="style-5a24e-kzdyurm3ju" data-absolute-path="http://edit.www.imcreator.com/viewer" >
 
<!-- ARRANGER SETTINGS -->
 
<!-- ARRANGER SETTINGS -->
 
<div class="arranger-settings" style="display:none;"
 
<div class="arranger-settings" style="display:none;"
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<!-- MENUS START -->
 
<!-- MENUS START -->
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<div  id="vbid-c148c-zyed6lpc"  class="master item-box  header-box style-c148c-oayvzg7x injected        " data-holder-type="header"" data-holder-type="header"  data-child-type="ORIGIN"  data-styleid="style-c148c-oayvzg7x" data-preview-styleid='style-c148c-oayvzg7x' data-preset-type-id="MENUS">
+
<div  id="vbid-59a5b-1xbnq2ek"  class="master item-box  header-box style-59a5b-jbqq3wgo injected        " data-holder-type="header"" data-holder-type="header"  data-child-type="ORIGIN"  data-styleid="style-59a5b-jbqq3wgo" data-preview-styleid='style-59a5b-jbqq3wgo' data-preset-type-id="MENUS">
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div class="header-wrapper item-wrapper menus-wrapper ">
 
<div class="header-wrapper item-wrapper menus-wrapper ">
 
 
−
<div class="item-content leaf menu_layout header content" data-preview-style="style-c148c-oayvzg7x" data-style="style-7f715-scjjarqgxu"  data-behavior-type="NOTHING" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-c148c-zyed6lpc" data-bgimg="">
+
<div class="item-content leaf menu_layout header content" data-preview-style="style-59a5b-jbqq3wgo" data-style="style-7f715-scjjarqgxu"  data-behavior-type="NOTHING" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-59a5b-1xbnq2ek" data-bgimg="">
 
<div class="preview-content-wrapper">
 
<div class="preview-content-wrapper">
 
<div class="preview-content-holder">
 
<div class="preview-content-holder">
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<!-- TITLE TEMPLATE -->
 
<!-- TITLE TEMPLATE -->
 
 
 +
<div class="preview-title-holder removable-parent">
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<h2 id="vbid-59a5b-0dca3gi2" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Menu</h2>
 +
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</div>
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<br />
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<!-- SUBTITLE TEMPLATE -->
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<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
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</div>
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</div>
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</div>
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<div class="right-div">
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<div class="benet" style="min-height:inherit;"></div>
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<!-- LINKS TEMPLATE -->
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<div class="preview-item-links ">
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<ul class="preview-links-wrapper">
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<li class="removable-parent">
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<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-qpimjeag" data-link-type="EXISTING"  target="_self" >
 +
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<span id="vbid-59a5b-w5gehayb"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team Members</span>
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</a>
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</li>
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<li class="removable-parent">
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<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-l5xeq0z6" data-link-type="EXISTING"  target="_self" >
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<span id="vbid-59a5b-9i6v7wly"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Projects</span>
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</a>
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</li>
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<li class="removable-parent">
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<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-xtfijafb" data-link-type="EXISTING"  target="_self" >
 +
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<span id="vbid-59a5b-b9vovddz"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
 +
</a>
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 +
</li>
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<li class="removable-parent">
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<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-bb9vg46d" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-59a5b-hlhlmo7j"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
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</a>
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</li>
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<li class="removable-parent">
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<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-hjemejig" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-59a5b-k2taajbu"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Human Practices</span>
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</a>
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</li>
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<li class="removable-parent">
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<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-0rawt2n3" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-59a5b-dv55z3u4"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Awards</span>
 +
</a>
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</li>
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 +
<li class="removable-parent">
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<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-szkh4ovx" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-59a5b-qli0rtxn"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Judging Form</span>
 +
</a>
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</li>
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<li class="removable-parent">
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<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-59a5b-egwntcng"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
 +
</a>
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</li>
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 +
 +
<li class="removable-parent">
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 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-akva45w2" data-link-type="EXISTING"  target="_self" >
 +
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<span id="vbid-59a5b-vcbsdeug"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Collaborations</span>
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</a>
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</li>
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<li class="removable-parent">
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<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-oiquicn3" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-59a5b-bnaeflqb"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Experiments</span>
 +
</a>
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 +
</li>
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<li class="removable-parent">
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<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-kmybr6mb" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-59a5b-c9f8qbif"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Design</span>
 +
</a>
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 +
</li>
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<li class="removable-parent">
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 +
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-xghz2dmu" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-59a5b-cxv3wrcd"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
 +
</a>
 +
 +
</li>
 +
  
 
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
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</ul>
 
</ul>
 
</div>
 
</div>
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<button class="hamburger links-menu-btn  small" type="button">
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  <span class="hamburger-box">
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  <span class="hamburger-inner"></span>
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  </span>
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</button>
 
 
 
 
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<!-- PROMO START -->
 
<!-- PROMO START -->
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<div  id="vbid-8d2b75a7-jnznmpah"  class="master item-box  gallery-box style-8d2b75a7-ahof2xa9       fill-height  " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-8d2b75a7-ahof2xa9" data-preview-styleid='style-8d2b75a7-ahof2xa9' data-preset-type-id="PROMO">
+
<div  id="vbid-59a5b-x4wfzsgg"  class="master item-box  gallery-box style-59a5b-erfb0r5q       fill-height  " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-59a5b-erfb0r5q" data-preview-styleid='style-59a5b-erfb0r5q' data-preset-type-id="PROMO">
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div class="gallery-wrapper item-wrapper promo-wrapper ">
 
<div class="gallery-wrapper item-wrapper promo-wrapper ">
 
 
−
<div  class="sub container style-8d2b75a7-ahof2xa9 content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-8d2b75a7-ahof2xa9" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-8d2b75a7-jnznmpah" data-preset-type-id="PROMO" data-preview-style="style-8d2b75a7-ahof2xa9" data-style="style-f3095-j0xhjgzynh" data-absolute-path="http://edit.www.imcreator.com/viewer" >
+
<!-- CACHED VERSION (7) OF vbid-59a5b-x4wfzsgg FROM  22-06-2017 19:56:44 --><div  class="sub container style-59a5b-erfb0r5q content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-59a5b-erfb0r5q" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-59a5b-x4wfzsgg" data-preset-type-id="PROMO" data-preview-style="style-59a5b-erfb0r5q" data-style="style-f3095-j0xhjgzynh" data-absolute-path="http://edit.www.imcreator.com/viewer" >
 
<!-- ARRANGER SETTINGS -->
 
<!-- ARRANGER SETTINGS -->
 
<div class="arranger-settings" style="display:none;"
 
<div class="arranger-settings" style="display:none;"
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−
<div  id="vbid-8d2b75a7-nbwucguy"  class="sub item-box  page-box style-8d2b75a7-ahof2xa9         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-8d2b75a7-ahof2xa9" data-preview-styleid='style-8d2b75a7-ahof2xa9' data-preset-type-id="UNRESOLVED">
+
<div  id="vbid-59a5b-oifoouiq"  class="sub item-box  page-box style-59a5b-erfb0r5q         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-erfb0r5q" data-preview-styleid='style-59a5b-erfb0r5q' data-preset-type-id="UNRESOLVED">
 
 
 
<div class="page-wrapper item-wrapper ">
 
<div class="page-wrapper item-wrapper ">
 
 
−
<div class="item-content leaf multi_layout page content -container" data-self="vbid-8d2b75a7-nbwucguy" data-preview-style="style-8d2b75a7-ahof2xa9" data-style="style-f3095-yq39oaomol" data-orig-thumb-height="3833" data-orig-thumb-width="1600" data-vbid="vbid-8d2b75a7-nbwucguy" data-bgimg="https://lh3.googleusercontent.com/VXIwTlJ-FRtPL2OEJWz3C0Y7UutFESnNNG8_ZeurITGttXG_GNUXRUVbDnwHei2ZMen4k0ZbfyEZaKOUGg">
+
<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-oifoouiq" data-preview-style="style-59a5b-erfb0r5q" data-style="style-f3095-yq39oaomol" data-orig-thumb-height="3833" data-orig-thumb-width="1600" data-vbid="vbid-59a5b-oifoouiq" data-bgimg="https://lh3.googleusercontent.com/VXIwTlJ-FRtPL2OEJWz3C0Y7UutFESnNNG8_ZeurITGttXG_GNUXRUVbDnwHei2ZMen4k0ZbfyEZaKOUGg">
 
<div  class="multi-container preview image-cover" >
 
<div  class="multi-container preview image-cover" >
 
<div class="Picture item-preview">
 
<div class="Picture item-preview">
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<div class="pic-side">
 
<div class="pic-side">
 
<div class="vertical-aligner">
 
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<h3 id="vbid-59a5b-cevoymcv" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Goal of Our Project</h3>
  
 
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<p class="MsoNormal"><span style="font-size:24px;"><span style="line-height: 115%; font-family: Verdana, sans-serif;">Our circuit was designed to utilize specific parts so that when the Ecoli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light. </span></span><o:p></o:p></p>
+
<p class="MsoNormal"><span style="font-size:24px;"><span style="line-height: 115%; font-family: Verdana, sans-serif;"><span style="font-family:montserrat;">Our circuit was designed to utilize specific parts so that when the Ecoli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.</span> </span></span><o:p></o:p></p>
 
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<span id="vbid-8d2b75a7-9xpok6ie"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >Read More</span>
 
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<div  class="sub container style-59a5b-oolaaxjn content matrix  " data-itemtype="folder" data-creator="" data-itemname="LOADING"  data-itemslug="loading" data-itemstyleid="style-59a5b-oolaaxjn" data-margintop="" data-arranger="matrix" data-layout="multi" data-vbid="vbid-59a5b-7sqagfhc" data-preset-type-id="TEAM" data-preview-style="style-59a5b-oolaaxjn" data-style="style-8ec4c-t1avbp6mmi" data-absolute-path="http://edit.www.imcreator.com/viewer" >
 
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<div  id="vbid-59a5b-gnbwjbto"  class="stripe-header sub item-box page-box style-59a5b-oolaaxjn       " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-oolaaxjn" data-preview-styleid='style-59a5b-oolaaxjn' data-preset-type-id="UNRESOLVED">
 
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+
<div class="item-content leaf blocks_layout page content" data-self="vbid-59a5b-gnbwjbto" data-preview-style="style-59a5b-oolaaxjn" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-59a5b-gnbwjbto" data-bgimg="">
 
<div class="helper-div middle" >
 
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<div class="blocks-preview-title-holder removable-parent order-handle">
 
 
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<h2 id="vbid-559f00b3-9gtfuba5" class="preview-element blocks-preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="BLOCKS_PREVIEW_TITLE" >Testing the circuit<br><br>• Before running the actual circuit, we ran control experiments
+
<h2 id="vbid-59a5b-gwjanbcf" class="preview-element blocks-preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="BLOCKS_PREVIEW_TITLE" >Testing the circuit<br><br>• Before running the actual circuit, we ran control experiments
 
to determine whether or not the GFP would indeed function as a reporter,
 
to determine whether or not the GFP would indeed function as a reporter,
 
meaning it can be regulated by a riboswitch<br>.
 
meaning it can be regulated by a riboswitch<br>.
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-6lnqgg1g" data-preview-style="style-59a5b-oolaaxjn" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="212" data-orig-thumb-width="230" data-vbid="vbid-59a5b-6lnqgg1g" data-bgimg="https://lh3.googleusercontent.com/fMlaYX5lH9NBFhTKVOCsFQaNnsk45b-Iz6PdOxECog_7kMWBfd_p6wSNVfZnJrSzuEeuqaAv3CnCU3Ou">
 
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<div  id="vbid-59a5b-tkb37uqz" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/fMlaYX5lH9NBFhTKVOCsFQaNnsk45b-Iz6PdOxECog_7kMWBfd_p6wSNVfZnJrSzuEeuqaAv3CnCU3Ou=s300);"  data-orig-width="230" data-orig-height="212" >
 
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<h2 id="vbid-559f00b3-bwglemsl" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >The original ticket before running</h2>
+
<h2 id="vbid-59a5b-bcqjpwgc" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >The original ticket before running</h2>
 
 
 
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<p style="line-height: 1.5em; font-size: 13px; font-family: Verdana, Arial, Helvetica, sans-serif; overflow: visible; background-color: rgb(224, 224, 224);"><span style="font-size:18px;"><span style="height: auto; width: auto; position: relative; color: rgb(105, 105, 105);"><span style="height: auto; width: auto; position: relative; font-family: arial, ArialRegular, helvetica, sans-serif;">Zen has a team of over 50 consultants, mostly with past experience in the energy industry. The team has deep knowledge of most aspects of the energy business, including particularly fuels and risk management.</span></span></span></p><p style="line-height: 1.5em; font-size: 13px; font-family: Verdana, Arial, Helvetica, sans-serif; overflow: visible; background-color: rgb(224, 224, 224);"><span style="height: auto; width: auto; position: relative; color: rgb(105, 105, 105);"><span style="height: auto; width: auto; position: relative; font-family: arial, ArialRegular, helvetica, sans-serif; font-size: 14px;"> </span></span></p>
+
<p style="line-height: 1.5em; overflow: visible; text-align: center;"><span style="line-height:2em;"><span style="font-family:montserrat;"><span style="font-size: 18px;"><span style="background-color:#FFFFFF;">Zen has a team of over 50 consultants, mostly with past experience in the energy industry. The team has deep knowledge of most aspects of the energy business, including particularly fuels and risk management.</span></span></span></span></p>
 
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<div  id="vbid-59a5b-7ew2skhe"  class="sub item-box  page-box style-59a5b-oolaaxjn         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-oolaaxjn" data-preview-styleid='style-59a5b-oolaaxjn' data-preset-type-id="UNRESOLVED">
 
 
 
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+
<div  id="vbid-59a5b-mqqqrgov" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/rEhUJjgx45G8SbWJWBTqcotjklMKZu5Zhvv5IraK4RNNpZyz9tmLMDHFpNmhE_-sMb-Rkm8FTG4BWEgt=s300);"  data-orig-width="231" data-orig-height="199" >
 
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<h2 id="vbid-559f00b3-oengnzoj" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Ticket after first control experiment </h2>
+
<h2 id="vbid-59a5b-zwmbrwsq" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Ticket after first control experiment </h2>
 
 
 
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+
<div id="vbid-59a5b-krhi6mkw" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
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<p style="line-height: 1.5em; font-size: 13px; font-family: Verdana, Arial, Helvetica, sans-serif; overflow: visible; background-color: rgb(224, 224, 224);"><span style="font-size:18px;"><span style="height: auto; width: auto; position: relative; color: rgb(105, 105, 105); line-height: 1.2; font-family: arial, ArialRegular, helvetica, sans-serif; background-color: transparent;">This experiment was the Constitutive GFP. The GFP was placed in the far left column, and only water was placed in the column next to it so that a control was present. BY comparing the far left column to the collum next to it, one can see the quantifiable difference between the wells with and without GFP</span></span></p><div><br></div>
+
<p class="MsoNormal" style="line-height: normal; text-align: center;"><span style="line-height:2em;"><span style="font-size:18px;"><span style="font-family:montserrat;">This experiment was the Constitutive GFP. The GFP was placed in the far left column, and only water was placed in the column next to it so that a control was present. BY comparing the far left column to the collum next to it, one can see the quantifiable difference between the wells with and without GFP</span></span></span><span style="font-size:8.0pt;
 +
font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"><o:p></o:p></span></p>
 
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<div  id="vbid-59a5b-baerbzkn"  class="sub item-box  page-box style-59a5b-oolaaxjn         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-oolaaxjn" data-preview-styleid='style-59a5b-oolaaxjn' data-preset-type-id="UNRESOLVED">
 
 
 
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<p style="line-height: 1.5em; font-size: 13px; font-family: Verdana, Arial, Helvetica, sans-serif; overflow: visible; background-color: rgb(224, 224, 224);"><span style="font-size:18px;"><span style="height: auto; width: auto; position: relative; color: rgb(105, 105, 105); line-height: 1.2; font-family: arial, ArialRegular, helvetica, sans-serif; background-color: transparent;">This experiments used the same controls in order to compare the GFP column to the control (water) column</span>.</span></p>
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Revision as of 19:59, 22 June 2017

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Cadets2Vets

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Goal of Our Project



Our circuit was designed to utilize specific parts so that when the Ecoli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.



Testing the circuit

• Before running the actual circuit, we ran control experiments to determine whether or not the GFP would indeed function as a reporter, meaning it can be regulated by a riboswitch
. • The first control experiment was constitutive: meaning that the GFP would always be produced. This was essentially only used to ensure that the GFP works in the paper ticket.
• The second control experiment was regulated via riboswitch SWH meaning that the GFP would be triggered. This tested if the GFP could be controlled/ regulated.
• The results from both of these experiments are shown in the pictures below



The original ticket before running



Zen has a team of over 50 consultants, mostly with past experience in the energy industry. The team has deep knowledge of most aspects of the energy business, including particularly fuels and risk management.



Ticket after first control experiment



This experiment was the Constitutive GFP. The GFP was placed in the far left column, and only water was placed in the column next to it so that a control was present. BY comparing the far left column to the collum next to it, one can see the quantifiable difference between the wells with and without GFP



Ticket after running second experiment



These experiments used the same controls in order to compare the GFP column to the control (water) column.


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