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The Hong Kong UCCKE team aims to create biobricks that detect and control gout by degrading uric acid and transport it. The Hong Kong UCCKE team presented their idea to us and we proposed that they can run an assay with plate reader measuring the fluorescence signal of the detection module, and incubating transformed E. coli in LB medium with saturated uric acid over night for the assay of the degradation module. Detailed protocols can be viewed here. | The Hong Kong UCCKE team aims to create biobricks that detect and control gout by degrading uric acid and transport it. The Hong Kong UCCKE team presented their idea to us and we proposed that they can run an assay with plate reader measuring the fluorescence signal of the detection module, and incubating transformed E. coli in LB medium with saturated uric acid over night for the assay of the degradation module. Detailed protocols can be viewed here. | ||
We have visited each others lab and presented our project in July, and we have suggested them to amplify the synthesized DNA from IDT for stock and use screening method other than restriction digestion(e.g TA clone) as the insert and vector have similar size. We also pointed the need of negative and positive controls in their assays. | We have visited each others lab and presented our project in July, and we have suggested them to amplify the synthesized DNA from IDT for stock and use screening method other than restriction digestion(e.g TA clone) as the insert and vector have similar size. We also pointed the need of negative and positive controls in their assays. | ||
− | Apart from providing advice and analysing the results, we also provided instrument for the standard cloning of their biobricks. As one of the secondary schools with advanced lab settings, they still lacks important devices like fridges for storing competent cell. Thus we helped them to clone | + | Apart from providing advice and analysing the results, we also provided instrument for the standard cloning of their biobricks. As one of the secondary schools with advanced lab settings, they still lacks important devices like -80C fridges for storing competent cell. Thus we helped them to clone biobricks in parallel. We also helped preparing the sequencing of their plasmids and perform the uric acid assay through plate reader for them. |
In return, the Hong Kong UCCKE team realised our hardware detection kit by translating our blueprint into 3d model and printed it for us. They also provided us suggestions on the improvement of the prototype regarding 3d printing production. The resulting product is as shown in the following figures.</p> | In return, the Hong Kong UCCKE team realised our hardware detection kit by translating our blueprint into 3d model and printed it for us. They also provided us suggestions on the improvement of the prototype regarding 3d printing production. The resulting product is as shown in the following figures.</p> | ||
Revision as of 12:28, 29 October 2017