Difference between revisions of "Team:XMU-China/Contribution"

Line 116: Line 116:
 
<span class="blank"></span>
 
<span class="blank"></span>
 
<span class="subtitle" id="subtitle2">-----* Experiment *-----</span>
 
<span class="subtitle" id="subtitle2">-----* Experiment *-----</span>
−
<p>Firstly, we got the biobrick called <strong>K592009</strong> from the <strong>2015kit</strong> and transfored it to the E.Coli DH5α. <strong>After a week</strong>, a big bacterial colony grown on the surface of plate.</p><br />
+
<p>Firstly, we got the biobrick called <strong>K1357009</strong> from the <strong>2017 DNA Distribution Kit Plates</strong> and transformed it to the E.Coli DH5α. <strong>After a one-week incubation</strong>, a big bacterial colony grown on the plate.</p><br />
 
<div class="contributionimg"><img class="contributionimg1" src="https://static.igem.org/mediawiki/2017/e/e9/T--XMU-China--contributionimg1.jpeg"></div><br /><br />
 
<div class="contributionimg"><img class="contributionimg1" src="https://static.igem.org/mediawiki/2017/e/e9/T--XMU-China--contributionimg1.jpeg"></div><br /><br />
−
<p>Secondly, we got new plasmid from the bacteria was recultured in the LB and transfored it to the E.Coli DH5α again. We observed the <strong>strong blue just one day later</strong>.</p><br />
+
<p>Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid J61002 with the promoter J23100, then transformed into E.coli DH5α again. We observed the <strong>strong blue just one day later</strong>. The result shows that BBa_K1357009 works well. </p><br />
 
<div class="contributionimg"><img class="contributionimg2" src="https://static.igem.org/mediawiki/2017/2/2a/T--XMU-China--contributionimg2.jpeg"></div>
 
<div class="contributionimg"><img class="contributionimg2" src="https://static.igem.org/mediawiki/2017/2/2a/T--XMU-China--contributionimg2.jpeg"></div>
 
<div class="contributionimg"><img class="contributionimg3" src="https://static.igem.org/mediawiki/2017/c/c1/T--XMU-China--contributionimg3.jpeg"></div>
 
<div class="contributionimg"><img class="contributionimg3" src="https://static.igem.org/mediawiki/2017/c/c1/T--XMU-China--contributionimg3.jpeg"></div>

Revision as of 12:53, 29 October 2017

2017.igem.org/Team:XMU-China/Contribution

-----* Introduce *-----

The fluorescent protein is used widly for a reporter,such as GFP and RFP. To enrich the kinds of fluorescent protein, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the coral Acropora millepora, amilCP. In this year, we want to verify the characteristic of BFP.

-----* Experiment *-----

Firstly, we got the biobrick called K1357009 from the 2017 DNA Distribution Kit Plates and transformed it to the E.Coli DH5α. After a one-week incubation, a big bacterial colony grown on the plate.




Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid J61002 with the promoter J23100, then transformed into E.coli DH5α again. We observed the strong blue just one day later. The result shows that BBa_K1357009 works well.