Difference between revisions of "Team:Cardiff Wales/Collaborations"

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ADD DATA FROM EXETER PROJECT.
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ALSO MENTION THAT SKYPED WITH XXXXX. OBTAINED CLONES FROM VALENCIA AND DISCUSSED PROJECT INFORMATON FROM WASHU WITH SUGGESTED EXPERIMENTS. THEY COULDN'T MAKE CLONES THAT WE COULD USE.</p>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<b> Exeter iGEM </b><br>
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After speaking to Exeter at the UK iGEM Meetup we arranged a collaboration. Initially, we had multiple ideas of how to collaborate, involving using some specialised equipment we had access to at Cardiff University. However, in the end we arranged an experiment to validate the results of an experiment that Exeter had previously carried out. Exeter were interested in testing the use of UV as a bactericide, as a way to ensure the biosafety of their filter model. Exeter sent us their protocol, which we followed and found results supporting their hypothesis.
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<h1>Collaborations</h1>
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
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<b> Valencia UPV </b> <br>
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Valencia UPV contacted us as both teams were working on similar expression systems, using tobacco plants. We began by setting up meetings over Skype to discuss our projects and begin to understand ways we could collaborate. We began discussing the different promoters both teams were trying to synthesise, and agreed that we could validate the promoter in one another’s expression system to confirm its function. However, due to time constraints we were unable to complete the experiment to test Valencia’s PR1:Luc+:NosT construct, and we encountered issues with the WRKY:Luc+:NosT construct we had aimed to send to Valencia, meaning we could not complete the collaboration. However, Although, speaking to Valencia UPV iGEM’s team proved very useful, as they have a lot of experience using plant expression systems, and during our skypes with them, we were able to ask them questions and understand where some of our experiments may have been going wrong.  
 
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<h3>Silver Medal Criterion #2</h3>
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Complete this page if you intend to compete for the silver medal criterion #2 on collaboration. Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.  
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<b>WashU </b><br>
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Early on in our project, we tweeted out offering collaboration by expressing other team’s systems in our plant expression system. Due to this tweet WashU iGEM got in touch with us, showing interest in expressing their project in plants. Their project aimed to reduce UV damage in photosynthetic organisms, but were unable to express the system in plants due to lack of specialist resources. We set up a skype meeting to talk about the collaboration, and what they would like us to do. However, WashU were unable to send the clones in the Golden Gate standard, that we needed for us to express their constructs in our plant expression system. Therefore, the collaboration was unable to be performed.  
 
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<h4> Which other teams can we work with? </h4>
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You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.
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In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
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Here are some suggestions for projects you could work on with other teams:
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<li> Improve the function of another team's BioBrick Part or Device</li>
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<li> Characterize another team's part </li>
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<li> Debug a construct </li>
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<li> Model or simulating another team's system </li>
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<li> Help build and test another team's hardware project</li>
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<li> Mentor a high-school team</li>
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Revision as of 13:31, 29 October 2017




Page Title




Exeter iGEM
After speaking to Exeter at the UK iGEM Meetup we arranged a collaboration. Initially, we had multiple ideas of how to collaborate, involving using some specialised equipment we had access to at Cardiff University. However, in the end we arranged an experiment to validate the results of an experiment that Exeter had previously carried out. Exeter were interested in testing the use of UV as a bactericide, as a way to ensure the biosafety of their filter model. Exeter sent us their protocol, which we followed and found results supporting their hypothesis.





Valencia UPV
Valencia UPV contacted us as both teams were working on similar expression systems, using tobacco plants. We began by setting up meetings over Skype to discuss our projects and begin to understand ways we could collaborate. We began discussing the different promoters both teams were trying to synthesise, and agreed that we could validate the promoter in one another’s expression system to confirm its function. However, due to time constraints we were unable to complete the experiment to test Valencia’s PR1:Luc+:NosT construct, and we encountered issues with the WRKY:Luc+:NosT construct we had aimed to send to Valencia, meaning we could not complete the collaboration. However, Although, speaking to Valencia UPV iGEM’s team proved very useful, as they have a lot of experience using plant expression systems, and during our skypes with them, we were able to ask them questions and understand where some of our experiments may have been going wrong.





WashU
Early on in our project, we tweeted out offering collaboration by expressing other team’s systems in our plant expression system. Due to this tweet WashU iGEM got in touch with us, showing interest in expressing their project in plants. Their project aimed to reduce UV damage in photosynthetic organisms, but were unable to express the system in plants due to lack of specialist resources. We set up a skype meeting to talk about the collaboration, and what they would like us to do. However, WashU were unable to send the clones in the Golden Gate standard, that we needed for us to express their constructs in our plant expression system. Therefore, the collaboration was unable to be performed.