Ryancoates (Talk | contribs) |
Ryancoates (Talk | contribs) |
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<p> A photo of the DNA gel that showed the primers used to isolate our promoters from <i> Arabidopsis </i> had worked. We then extracted the DNA out of the gel, and used these for subsequent reactions. These are <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404002"> PDF1.2, </a> <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404003"> PR2, </a>, <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404004">GST6, </a> and <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404005">WKRKY30 </a> (not shown).<br><br><br><br> | <p> A photo of the DNA gel that showed the primers used to isolate our promoters from <i> Arabidopsis </i> had worked. We then extracted the DNA out of the gel, and used these for subsequent reactions. These are <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404002"> PDF1.2, </a> <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404003"> PR2, </a>, <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404004">GST6, </a> and <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404005">WKRKY30 </a> (not shown).<br><br><br><br> | ||
<img src="https://static.igem.org/mediawiki/2017/a/aa/T--Cardiff_Wales--WRKYLvl0GEL.PNG"/><br><br> | <img src="https://static.igem.org/mediawiki/2017/a/aa/T--Cardiff_Wales--WRKYLvl0GEL.PNG"/><br><br> | ||
− | <p> A photo of a gel showing successful level 0 <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404005">WKRKY30 </a> constructs.<br><br><br><br> | + | <p> A photo of a gel showing successful level 0 <a href="http://parts.igem.org/partsdb/part_info.cgi?part_name=BBa_K2404005">WKRKY30 promoter</a> constructs. Unfortunately, this is as far as WRKY30 got, as all subsequent level 1 reactions failed (gels not shown).<br><br><br><br> |
<img src="https://static.igem.org/mediawiki/2017/2/21/T--Cardiff_Wales--PromoterLuc%2BDNA.PNG"/><br><br> | <img src="https://static.igem.org/mediawiki/2017/2/21/T--Cardiff_Wales--PromoterLuc%2BDNA.PNG"/><br><br> | ||
<p> A DNA gel confirming three of our 8 level 1 constructs. These were the only Luc+ level 1 parts that Team Plant Promoter managed to successfully create, but when <i> E. coli </i> were transformed with this constructs, they continually failed to grow. This resulted in us running out of time in the lab, so sadly it was the furthest stage that our inducible promoter:Luc+:NosT constructs got to.<br><br><br><br> | <p> A DNA gel confirming three of our 8 level 1 constructs. These were the only Luc+ level 1 parts that Team Plant Promoter managed to successfully create, but when <i> E. coli </i> were transformed with this constructs, they continually failed to grow. This resulted in us running out of time in the lab, so sadly it was the furthest stage that our inducible promoter:Luc+:NosT constructs got to.<br><br><br><br> |
Revision as of 14:27, 29 October 2017