Currently, amphibians with Chytridiomycosis must be treated in a lab environment and treating entire populations of amphibians is impossible. Laboratory treatments for Chytridiomycosis include killing Bd by heating the affected animal to 37 °C for 4 hours, but some amphibians are too sensitive to survive this treatment. Another treatment is with an antifungal ointment such as Itroconazole either through bathing or oral administration. More research is needed to know whether antifungal ointments are safe to use on certain amphibians.

The UFlorida iGEM team seeks to develop a new treatment for Chytridiomycosis based on the metabolites produced by the symbiotic bacteria of the amphibian populations resistant to the fungus. Tryptophol, a metabolite of a synergistic frog skin bacteria species, has been shown to be effective at combating the Batrachochytrium dendrobatidis fungus at low doses. Our team will modify E.coli with the genes comprising the pathway for tryptophol synthesis. The biosynthesis pathway for tryptophol begins with tryptophan as the substrate. Tryptophan is converted into (indol-3-yl)pyruvate by the enzyme Aromatic Amino Acid Aminotransferase II, which is coded for by the ARO9 gene in Saccharomyces cerevisiae. (Indole-3-yl)pyruvate is then converted into indole acetaldehyde by the enzyme 2-oxo Acid Decarboxylase, which is encoded by the ARO10 gene. Lastly, Indole acetaldehyde is converted into indole-3-ethanol (tryptophol) by the enzyme Alcohol Dehydrogenase I, which is encoded by the gene ADH1. In order to produce tryptophol from tryptophan, we will transform the ARO9, ARO10, and ADH1 genes into E.coli.

Our team chose to pursue this project because amphibian populations are an essential component to many ecosystems, especially as indicator species (which are known as early warning signs of declining health of the ecosystem). The amphibian populations in Florida are experiencing a steep decline, which could impact the rest of the ecosystem. In particular, amphibians help to control mosquito populations, which is a paramount issue in Florida. While the primary cause of the population decline is not known, Chytridiomycosis has been detected in Florida and remains a threat to the local amphibian populations.