Difference between revisions of "Team:Lambert GA"
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| − | <h2 style="margin=auto; margin-left=700px; color: #7A7A79;"> Who We Are </h2><p style=" margin-left=300px; color: #7A7A79;"> We are | + | <h2 style="margin=auto; margin-left=700px; color: #7A7A79;"> Who We Are </h2><p style=" margin-left=300px; color: #7A7A79;"> We are comprised of 14 high school students from Suwanee, Georgia in the 10th, 11th, and 12th grades. We are an after-school/before-school club that meets simply for the love of synthetic biology. |
</p><a style="text-decoration: none;" href="https://2017.igem.org/Team:Lambert_GA/Team"><button class="button button1">Find Out More</button></a> | </p><a style="text-decoration: none;" href="https://2017.igem.org/Team:Lambert_GA/Team"><button class="button button1">Find Out More</button></a> | ||
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<div align="right" ><h2 class="smallt" style="color: #FFFFFF;"> Our Project</h2></div> | <div align="right" ><h2 class="smallt" style="color: #FFFFFF;"> Our Project</h2></div> | ||
| − | <p style=" margin-left=300px;color: white;"> As an underfunded lab, our project aimed to reduce costs of lab work. While characterizing non-lysosomal inducible protein degradation, we developed the | + | <p style=" margin-left=300px;color: white;"> As an underfunded lab, our project aimed to reduce costs of lab work. While characterizing non-lysosomal inducible protein degradation, we developed the Chrome-Q, to quantify the degradation of protein. |
</p><a style="text-decoration: none;" href="https://2017.igem.org/Team:Lambert_GA/Description"><button class="button button2">Find Out More</button></a><br><br><br><br></div> | </p><a style="text-decoration: none;" href="https://2017.igem.org/Team:Lambert_GA/Description"><button class="button button2">Find Out More</button></a><br><br><br><br></div> | ||
Revision as of 21:52, 29 October 2017
Characterizing Non-Lysosomal Inducible Protein Degradation
In the development of genetic circuits, researchers often face issues with the overlap of protein expression. As a result, the 2017 Lambert iGEM team aimed to develop a clean way to “switch” off protein expression by further characterizing a proteolytic mechanism known as ClpXP. An inducible genetic construct was made to express tsPurple (a chromoprotein) and degrade via ClpXP upon induction of varying levels of IPTG, resulting in correlating amounts of protein degradation. Data was collected on the team’s engineered Chrom-Q, a 3-D printed camera-device that supports a constant light source for centrifuged cells; in turn the data was analyzed using Lambert iGEM's self-constructed software app to determine HSL values. The purpose and goal for this technology was to promote scientific research under any financial circumstance to quantify data in standardized conditions. Measuring relative strengths of protein degradation using self-engineered products will allow an economic approach in characterizing non-lysosomal proteolysis.
Who We Are
We are comprised of 14 high school students from Suwanee, Georgia in the 10th, 11th, and 12th grades. We are an after-school/before-school club that meets simply for the love of synthetic biology.
