Difference between revisions of "Team:XJTLU-CHINA/Lab book"
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<li>Transformation of pUC57-DRGN-1 into <i>E.coli</i> Top10</li> | <li>Transformation of pUC57-DRGN-1 into <i>E.coli</i> Top10</li> | ||
<li>Grow pMG36e-transformed <i>E.coli</i> in LB broth</li> | <li>Grow pMG36e-transformed <i>E.coli</i> in LB broth</li> | ||
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<p>6.23 </p> | <p>6.23 </p> | ||
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Latest revision as of 23:26, 29 October 2017
Lab Book
Molecular experiments notes of our project
Experiments overview spanning the project
June
6.21
- Transformation of pMG36e into E.coli Top10
6.22
- Transformation of pUC57-DRGN-1 into E.coli Top10
- Grow pMG36e-transformed E.coli in LB broth
6.23
- Isolation of pMG36e plasmid from bacteria solution
- Grow DRGN-1 transformed E.coli in LB broth
- Run agarose gel for pMG36e. Result: no target band
6.24
- Isolation of pUC57-DRGN-1 from bacteria solution
- Double-enzyme digestion of isolated pUC57-DRGN-1 using EcoRI and PstI.
- Run agarose gel for confirmation. Result: confirmed.
6.25
- Repeat the amplification experiment of pMG36e
- Preparation of DH5ɑ competent cells
6.26
- Select single colony of pMG36e-transformed E. coli, and transfer in LB broth.
- Amplify the E. coli DH5ɑ containing pNZ8148 plasmid
6.27
- The extraction of pMG36e and pNZ8148 plasmid
- Digestion of pNZ8148 using XbaI
- Agarose gel electrophoresis of digested pNZ8148. Result: confirmed, and the extracted plasmid is in super-coiled form
6.30
- Gel electrophoresis for pMG36e, pUC57-DRGN-1 and pUC19. Results：no band for pMG36e, the other two are successful.
Interlab experiments
Collaborators and Supporters
