Difference between revisions of "Team:XJTLU-CHINA/Notebook"

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  <h1 style="color:white; font-size:80px; padding-top:120px;">Lab Book</h1>
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  </div>
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<div class="container">
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    <h1>Molecular experiments notes of our project</h1>
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    <h2>Experiments overview spanning the project</h2>
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    <h3>June</h3>
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    <p>6.21</p>
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    <ol>
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    <li>Transformation of pMG36e into <i>E.coli</i> Top10</li>
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    </ol>
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    <p>6.22</p>
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    <ol>
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<li>Transformation of pUC57-DRGN-1 into <i>E.coli</i> Top10</li>
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<li>Grow pMG36e-transformed <i>E.coli</i> in LB broth</li>
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</ol>
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<p>6.23 </p>
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<ol>
 +
<li>Isolation of pMG36e plasmid from bacteria solution</li>
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<li>Grow DRGN-1 transformed <i>E.coli</i> in LB broth</li>
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<li>Run agarose gel for pMG36e. Result: no target band</li>
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</ol>
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<p>6.24</p>
 +
<ol>
 +
<li>Isolation of pUC57-DRGN-1 from bacteria solution</li>
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<li>Double-enzyme digestion of isolated pUC57-DRGN-1 using EcoRI and PstI.</li>
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<li>Run agarose gel for confirmation. Result: confirmed.</li>
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</ol>
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<p>6.25</p>
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<ol>
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<li>Repeat the amplification experiment of pMG36e</li>
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<li>Preparation of DH5ɑ competent cells</li>
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</ol>
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<p>6.26</p>
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<ol>
 +
<li>Select single colony of pMG36e-transformed <i>E. coli</i>, and transfer in LB broth.</li>
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<li>Amplify the E. coli DH5ɑ containing pNZ8148 plasmid</li>
 +
</ol>
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<p>6.27</p>
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<ol>
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<li>The extraction of pMG36e and pNZ8148 plasmid</li>
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<li>Digestion of pNZ8148 using XbaI</li>
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<li>Agarose gel electrophoresis of digested pNZ8148. Result: confirmed, and the extracted plasmid is in super-coiled form</li>
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</ol>
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<p>6.30</p>
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<ol>
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<li>Gel electrophoresis for pMG36e, pUC57-DRGN-1 and pUC19. Results：no band for pMG36e, the other two are successful.</li>
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</ol>
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<hr>
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    <h1>Interlab experiments</h1>
  
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<div class="column full_size">
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</div><!-- container --!>
  
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<h1>Notebook</h1>
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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<!-- banner -->
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<div class="text-center" style="background:#cfe5d9;">
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<h1 style="font-weight:bolder;color:#006934; ">Collaborators and Supporters</h1>
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<div class="container-fluid supporters-logos">
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<div class="row">
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    <div class="col-sm-4"> 
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        <a href="https://www.synbio-tech.com.cn"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/3/38/Synbio_tech_logo.png"></a>
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    </div>
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    <div class="col-sm-4"> 
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        <a href="https://www.wx2h.com/web/index.php"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/a/ab/Wuxi_No.2_people%27s_hospital.png"></a>
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    </div>   
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    <div class="col-sm-4"> 
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        <a href="https://www.chinapeptides.qianyan.biz"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/e/e8/Qiang_yao_sheng_wu.png"></a>
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    </div>
  
 
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</div>
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<div class="clear"></div>
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<div class="row">
  
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    <div class="col-sm-4"> 
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        <a href="https://www.neb.com"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/0/06/NEB_logo.png"></a>
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    </div>
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    <div class="col-sm-4">
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        <a href="https://www.snapgene.com"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/c/cb/Snapgene_logo.png"></a>
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    </div>
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    <div class="col-sm-4">
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        <a href="https://www.genscript.com"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/9/9b/Genscript.png"></a>
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    </div>   
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</div>   
  
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<h5>What should this page have?</h5>
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</div><!-- /.text-center --!>
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<ul>
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<li>Chronological notes of what your team is doing.</li>
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<li> Brief descriptions of daily important events.</li>
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<li>Pictures of your progress. </li>
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<li>Mention who participated in what task.</li>
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</ul>
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<!-- footer -->
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<footer>
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<div class="text-center">
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    <div class="container-fluid">
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        <div class="row">
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          <div class="col-md-4 loaction">
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              <h4>Location</h4>
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              <p  style="text-align:center;">Rm 363, Science Building<br>
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              Xi'an Jiaotong-Liverpool University<br>
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              111 Ren'ai Road, Suzhou, China<br>
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              215123
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              </p>
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          </div>
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          <div class="col-md-4 social">
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              <h4>Social</h4>
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              <a href=""><img src="https://static.igem.org/mediawiki/2017/9/9f/XJTLU_facebook.png" alt="facebook" width=30 height=30></a>
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              <a href=""><img src="https://static.igem.org/mediawiki/2017/7/72/XJTLU_blog.png" alt="blog" width=30 height=30></a>
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          </div>
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          <div class="col-md-4 contact">
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              <h4>Get in touch</h4>
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              <img src="https://static.igem.org/mediawiki/2017/1/19/XJTLU_email.png" alt="emali" width=30 height=30>
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              <p style="text-align:center;">igem@xjtlu.edu.cn</p>
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          </div>
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        </div>
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    </div>
 
</div>
 
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<div class="text-center" style="background:#003b73;">
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        <p style="text-align:center; color:white;">XJTLU-CHINA iGEM 2017</p>
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<h5>Inspiration</h5>
 
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<p>You can see what others teams have done to organize their notes:</p>
 
  
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<ul>
 
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
 
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</ul>
 
  
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</div>
 
 
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Revision as of 23:29, 29 October 2017

Lab book

Lab Book

Molecular experiments notes of our project

Experiments overview spanning the project

June

6.21

  1. Transformation of pMG36e into E.coli Top10

6.22

  1. Transformation of pUC57-DRGN-1 into E.coli Top10
  2. Grow pMG36e-transformed E.coli in LB broth

6.23

  1. Isolation of pMG36e plasmid from bacteria solution
  2. Grow DRGN-1 transformed E.coli in LB broth
  3. Run agarose gel for pMG36e. Result: no target band

6.24

  1. Isolation of pUC57-DRGN-1 from bacteria solution
  2. Double-enzyme digestion of isolated pUC57-DRGN-1 using EcoRI and PstI.
  3. Run agarose gel for confirmation. Result: confirmed.

6.25

  1. Repeat the amplification experiment of pMG36e
  2. Preparation of DH5ɑ competent cells

6.26

  1. Select single colony of pMG36e-transformed E. coli, and transfer in LB broth.
  2. Amplify the E. coli DH5ɑ containing pNZ8148 plasmid

6.27

  1. The extraction of pMG36e and pNZ8148 plasmid
  2. Digestion of pNZ8148 using XbaI
  3. Agarose gel electrophoresis of digested pNZ8148. Result: confirmed, and the extracted plasmid is in super-coiled form

6.30

  1. Gel electrophoresis for pMG36e, pUC57-DRGN-1 and pUC19. Results：no band for pMG36e, the other two are successful.

Interlab experiments

Collaborators and Supporters

Location

Rm 363, Science Building
Xi'an Jiaotong-Liverpool University
111 Ren'ai Road, Suzhou, China
215123

Get in touch

emali

igem@xjtlu.edu.cn

XJTLU-CHINA iGEM 2017