Line 151: | Line 151: | ||
<li>Start the cycle and wait till it is finished. </li> | <li>Start the cycle and wait till it is finished. </li> | ||
</ol> | </ol> | ||
+ | <a href="https://www.neb.com/protocols/1/01/01/pcr-protocol-m0530">Click here for the manufacturer's maual</a> | ||
</p> | </p> | ||
Line 183: | Line 184: | ||
<li>Incubate overnight at 37°C with plates upside down.</li> | <li>Incubate overnight at 37°C with plates upside down.</li> | ||
</ol> | </ol> | ||
+ | <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846332/">Click here for the manufacturer's maual</a> | ||
</div> | </div> | ||
Line 212: | Line 214: | ||
<li>Add 30 μl of distilled water to elute DNA.</li> | <li>Add 30 μl of distilled water to elute DNA.</li> | ||
</ol> | </ol> | ||
+ | <a href=" http://eng.intronbio.com/PROTO-PDF/mega-spin.pdf">Click here for the manufacturer's maual</a> | ||
</div> | </div> | ||
Line 261: | Line 264: | ||
<li>Send 5 μl of the ligated sample should be used for agarose gel electrophoresis to confirm whether ligation has occurred (Optional)</li> | <li>Send 5 μl of the ligated sample should be used for agarose gel electrophoresis to confirm whether ligation has occurred (Optional)</li> | ||
</ol> | </ol> | ||
+ | <a href=" https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0012178_XbaI_10_UuL_5X1500U_UG.pdf">Click here for the manufacturer's maual</a> | ||
</p> | </p> | ||
Line 308: | Line 312: | ||
<li>Purify the DNA by PCR purification kit/gel extraction kit for downstream process. </li> | <li>Purify the DNA by PCR purification kit/gel extraction kit for downstream process. </li> | ||
</ol> | </ol> | ||
+ | <a href=" https://www.neb.com/protocols/0001/01/01/dna-ligation-with-t4-dna-ligase-m0202">Click here for the manufacturer's maual</a> | ||
</p> | </p> | ||
Line 373: | Line 378: | ||
<li>Test the transformation efficiency of competent cells with antibiotics resistance plamids at different concentrations.</li> | <li>Test the transformation efficiency of competent cells with antibiotics resistance plamids at different concentrations.</li> | ||
</ol> | </ol> | ||
− | + | <a href=" https://www.neb.com/protocols/2012/06/21/making-your-own-chemically-competent-cells">Click here for the manufacturer's maual</a> | |
</p> | </p> | ||
Line 413: | Line 418: | ||
<li>Incubate overnight at 37°C with plates upside down.</li> | <li>Incubate overnight at 37°C with plates upside down.</li> | ||
</ol> | </ol> | ||
+ | <a href=" https://www.neb.com/protocols/2012/05/21/transformation-protocol">Click here for the manufacturer's maual</a> | ||
</div> | </div> | ||
Line 448: | Line 454: | ||
<li>Centrifuge at 13,000 rpm for 1 min.</li> | <li>Centrifuge at 13,000 rpm for 1 min.</li> | ||
</ol> | </ol> | ||
− | + | <a href=" http://eng.intronbio.com/PROTO-PDF/DNA-spin.pdf">Click here for the manufacturer's maual</a> | |
</p> | </p> | ||
</div> | </div> | ||
Line 492: | Line 498: | ||
</ul> | </ul> | ||
</ol> | </ol> | ||
− | + | <a href=" https://www.thermofisher.com/hk/en/home/life-science/cell-culture/microbiological-culture/bacterial-growth-media/lb-broth-and-lb-agar.html">Click here for the manufacturer's maual</a> | |
</div> | </div> | ||
</div> | </div> | ||
Line 553: | Line 559: | ||
<li>Analyze the results of the reaction. </li> | <li>Analyze the results of the reaction. </li> | ||
</ol> | </ol> | ||
− | + | <a href=" https://www.promega.com/-/media/files/resources/protocols/technical-manuals/0/s30-t7-high-yield-protein-expression-system-protocol.pdf">Click here for the manufacturer's maual</a> | |
</p> | </p> | ||
</div> | </div> |
Revision as of 04:56, 30 October 2017