Difference between revisions of "Team:ETH Zurich/Parts"

Line 139: Line 139:
 
</figure>
 
</figure>
 
<p>Description.</p>
 
<p>Description.</p>
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500008">BBa_K2500008</a></p>
+
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500000">BBa_K2500000</a></p>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
<summary>BBa_K2500000: Bacterioferritin</summary>
+
<summary>BBa_K2500001: Azurin</summary>
 
<figure>
 
<figure>
 
<img src="#" alt="FIXME">
 
<img src="#" alt="FIXME">
 
</figure>
 
</figure>
 
<p>Description.</p>
 
<p>Description.</p>
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500008">BBa_K2500008</a></p>
+
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500001">BBa_K2500001</a></p>
 
</details>
 
</details>
−
       
+
 
 
<details>
 
<details>
−
            <summary>BBa_K2500008: Pconst_RBSeng_TlpA</summary>
+
<summary>BBa_K2500002: p28</summary>
 +
<figure>
 +
<img src="#" alt="FIXME">
 +
</figure>
 +
<p>Description.</p>
 +
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500002">BBa_K2500002</a></p>
 +
</details>
  
−
            <figure>
+
<details>
−
                <img src="#" alt="FIXME">
+
<summary>BBa_K2500003: p<sub>TlpA</sub></summary>
−
            </figure>
+
<figure>
 +
<img src="#" alt="FIXME">
 +
</figure>
 +
<p>Description.</p>
 +
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500003">BBa_K2500003</a></p>
 +
</details>
  
−
            <p>Description.</p>
+
<details>
 +
<summary>BBa_K2500004: TlpA</summary>
 +
<figure>
 +
<img src="#" alt="FIXME">
 +
</figure>
 +
<p>Description.</p>
 +
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500004">BBa_K2500004</a></p>
 +
</details>
  
−
            <p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500008">BBa_K2500008</a></p>
+
<details>
−
        </details>
+
<summary>BBa_K2500005: RBS_TlpA</summary>
 +
<figure>
 +
<img src="#" alt="FIXME">
 +
</figure>
 +
<p>Description.</p>
 +
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500005">BBa_K2500005</a></p>
 +
</details>
  
−
        <details>
+
<details>
−
            <summary>BBa_K2500010: AND gate a</summary>
+
<summary>BBa_K2500006: Protein E</summary>
 +
<figure>
 +
<img src="#" alt="FIXME">
 +
</figure>
 +
<p>Description.</p>
 +
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500006">BBa_K2500006</a></p>
 +
</details>
  
−
            <figure class="A">
+
<details>
−
                <img src="https://static.igem.org/mediawiki/2017/7/72/T--ETH_Zurich--ANDgateA.png" alt="FIXME">
+
<summary>BBa_K2500007: RBS<sub>eng</sub>_TlpA</summary>
−
            </figure>
+
<figure>
 +
<img src="#" alt="FIXME">
 +
</figure>
 +
<p>Description.</p>
 +
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500007">BBa_K2500007</a></p>
 +
</details>
  
−
            <p>In absence of high concentrations of L-lactate, LldR inhibitor proteins bind to the binding sites O1 and O2 surrounding the pLux promoter leading to the formation of a DNA loop. The pLux promoter is sequestered and inaccessible for transcription. In design a, the distances between the intercalated promoter and the binding sites were adapted from <a href="http://parts.igem.org/Part:BBa_K1847007">BBa_K1847007</a>.</p>
+
       
 +
<details>
 +
<summary>BBa_K2500008: p<sub>Const</sub>_RBS_LldP/LldR_p<sub>Const</sub>_RBS_LuxR</summary>
 +
<figure>
 +
<img src="#" alt="FIXME">
 +
</figure>
 +
<p>Description.</p>
 +
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500008">BBa_K2500008</a></p>
 +
</details>
  
−
            <p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500010">BBa_K2500010</a></p>
+
       
−
        </details>
+
 
 +
<details>
 +
<summary>BBa_K2500009: RBS<sub>eng</sub>_ProteinE</summary>
 +
<figure>
 +
<img src="#" alt="FIXME">
 +
</figure>
 +
<p>Description.</p>
 +
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500009">BBa_K2500009</a></p>
 +
</details>
 +
 
 +
 
 +
<details>
 +
<summary>BBa_K2500010: AND gate A</summary>
 +
<figure class="A">
 +
<img src="https://static.igem.org/mediawiki/2017/7/72/T--ETH_Zurich--ANDgateA.png" alt="FIXME">
 +
</figure>
 +
<p>In absence of high concentrations of L-lactate, LldR inhibitor proteins bind to the binding sites O1 and O2 surrounding the pLux promoter leading to the formation of a DNA loop. The pLux promoter is sequestered and inaccessible for transcription. In design a, the distances between the intercalated promoter and the binding sites were adapted from <a href="http://parts.igem.org/Part:BBa_K1847007">BBa_K1847007</a>.</p>
 +
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500010">BBa_K2500010</a></p>
 +
</details>
  
  

Revision as of 08:59, 30 October 2017

Parts

FIXME

Overview

We submitted in total 14 new BioBricks to the iGEM registry! You can find all parts and their design below.

Basic Parts

Part Description BioBrick
Bacterioferritin Heme-deletion mutant of bacterial iron storage protein functioning as an MRI contrast agent BBa_K2500000
Azurin Redox protein originating from P. aeruginosa with anti-cancer activity BBa_K2500001
p28 Effector domain of azurin BBa_K2500002
pTlpA Temperature-responsive promoter optimized for slight activation above 37°C and full activation at 45 °C BBa_K2500003
TlpA Temperature-dependent transcriptional repressor of pTlpa BBa_K2500004
RBS_TlpA Temperature-dependent transcriptional repressor of pTlpa and synthetic RBS BBa_K2500005
Protein E Bacteria-lysing protein encoded by phage Phi X 17 BBa_K2500006
RBSeng_TlpA Temperature-dependent transcriptional repressor of pTlpa with engineered RBS BBa_K2500007
RBSeng_ProteinE Bacteria-lysing protein encoded by phage Phi X 174 with engineered RBS BBa_K2500009
AND gate A Synthetic promoter responsive to LldR and luxR BBa_K2500010
Best Basic Part:
AND gate B		 Synthetic promoter responsive to LldR and luxR BBa_K2500011
AND gate C Synthetic promoter responsive to LldR and luxR BBa_K2500012
pConst_RBS_LldP/
LldR_pConst_RBS_LuxR		 Expression cassette consisting of LuxR and LldP/LldR BBa_K2500013

Composite Parts

Part Description BioBrick
pConstRBSeng_TlpA Constitutively expressed temperature-dependent transcriptional repressor of pTlpa with engineered RBS BBa_K2500008

Design

BBa_K2500000: Bacterioferritin
FIXME

Description.

Registry: BBa_K2500000

BBa_K2500001: Azurin
FIXME

Description.

Registry: BBa_K2500001

BBa_K2500002: p28
FIXME

Description.

Registry: BBa_K2500002

BBa_K2500003: pTlpA
FIXME

Description.

Registry: BBa_K2500003

BBa_K2500004: TlpA
FIXME

Description.

Registry: BBa_K2500004

BBa_K2500005: RBS_TlpA
FIXME

Description.

Registry: BBa_K2500005

BBa_K2500006: Protein E
FIXME

Description.

Registry: BBa_K2500006

BBa_K2500007: RBSeng_TlpA
FIXME

Description.

Registry: BBa_K2500007

BBa_K2500008: pConst_RBS_LldP/LldR_pConst_RBS_LuxR
FIXME

Description.

Registry: BBa_K2500008

BBa_K2500009: RBSeng_ProteinE
FIXME

Description.

Registry: BBa_K2500009

BBa_K2500010: AND gate A
FIXME

In absence of high concentrations of L-lactate, LldR inhibitor proteins bind to the binding sites O1 and O2 surrounding the pLux promoter leading to the formation of a DNA loop. The pLux promoter is sequestered and inaccessible for transcription. In design a, the distances between the intercalated promoter and the binding sites were adapted from BBa_K1847007.

Registry: BBa_K2500010

BBa_K2500011: AND gate b

FIXME

In design b, each binding site was duplicated in order to achieve a potential zipper mechanism and stronger inhibition due to binding more LldR inhibitor proteins.

Registry: BBa_K2500011

BBa_K2500012: AND gate c
FIXME

In design c, an artificial spacer was embedded between the pLux promoter and the O2 binding site in order to influence the looping dynamics.

Registry: BBa_K2500012