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− | We measured the cell density (OD600 values) and fluorescence signal of the cell culture at | + | We measured the cell density (OD600 values) and fluorescence signal of the cell culture at 2-hour interval. We processed the data by averaging the obtained values from two flasks of culture of different colonies with the same device. The results were represented in the graph below: |
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− | Among the devices that share the RBS B0034 ( | + | Among the devices that share the RBS B0034 (devices 1, 2, 3), device 1 has the highest fluorescence per OD600 and device 3 has the lowest. The difference in fluorescence per OD600 is probably due to the promoter strength. Device 1 has the strongest promoter(J23101), thus it has the highest GFP production rate and give the highest fluorescence. Device 3 has the weakest promoter(J23117) , thus it has the lowest GFP production rate and give the lowest fluorescence. The strength of promoter can also be deduced by comparing the fluorescence per OD600 given by the devices that share the BCD2 (devices 4, 5, 6). Device 4 has the strongest fluorescent signal because it uses the strongest promoter (J23101), while device 6 has a weakest fluorescent signal because the weakest promoter(J23117) is used. |
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− | Comparing the fluorescence signal given by devices with the same promoter but different RBS, we observed that the devices that use BCD2 (devices 4,5 and 6) gave a lower fluorescence comparing with the devices that use B0034 (devices 1, 2 and 3). We may therefore conclude that BCD2 | + | Comparing the fluorescence signal given by devices with the same promoter but different RBS, we observed that the devices that use BCD2 (devices 4,5 and 6) gave a lower fluorescence comparing with the devices that use B0034 (devices 1, 2 and 3). We may therefore conclude that BCD2 has a weaker RBS than B0034. |
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− | Another interesting point is the fluorescence per cell of all devices peaked at (t=2 | + | Another interesting point is the fluorescence per cell of all devices peaked at (t=2 hour). These may because the increase in cell number is much faster than the overall expression of GFP after t=2 hour. |
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Revision as of 10:00, 30 October 2017