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− | To conduct synthetic biology research, underfunded labs often lack the monetary means of buying expensive laboratory equipment, such as a plate reader to measure fluorescence of cells or a centrifuge to pellet cells. These challenges are often insurmountable for small research facilities or scientists in the field. The approach Lambert iGEM used was multifaceted; design a chamber to optimize and standardize conditions for detecting color, write a software application that can be used by anyone to quantify the color values using HSL, test and improve on a low cost centrifuge, assemble and test these devices with chromoproteins under varying levels of IPTG induction. </p>Using engineering design principles, the 2017 Lambert iGEM team aimed to develop an imaging device called the Chrome-Q to cost-effectively standardize conditions for quantifying chromoprotein data; additionally, the team improved upon the Paperfuge originally designed by the Prakash Lab to pellet cells at a low cost. Different chromoproteins - Tinsel Purple, Virginia Violet and Scrooge Orange - were induced at varying levels of IPTG, causing the cells to express different intensities; pictures of the cells were taken using a | + | To conduct synthetic biology research, underfunded labs often lack the monetary means of buying expensive laboratory equipment, such as a plate reader to measure fluorescence of cells or a centrifuge to pellet cells. These challenges are often insurmountable for small research facilities or scientists in the field. The approach Lambert iGEM used was multifaceted; design a chamber to optimize and standardize conditions for detecting color, write a software application that can be used by anyone to quantify the color values using HSL, test and improve on a low cost centrifuge, assemble and test these devices with chromoproteins under varying levels of IPTG induction. </p>Using engineering design principles, the 2017 Lambert iGEM team aimed to develop an imaging device called the Chrome-Q to cost-effectively standardize conditions for quantifying chromoprotein data; additionally, the team improved upon the Paperfuge originally designed by the Prakash Lab to pellet cells at a low cost. Different chromoproteins - Tinsel Purple, Virginia Violet and Scrooge Orange - were induced at varying levels of IPTG, causing the cells to express different intensities; pictures of the cells were taken using a Chrome-Q and were measured with a self-constructed software app that analyzes the pigments using HSV color space. Additionally, the team observed that a common issue encountered by synthetic biologists is the overlap of protein expression in development of genetic circuits which negatively affects the quality of performance in a given cell. The 2017 Lambert iGEM Team worked to develop an inducible “switch” to further characterize ClpXP, a non-lysosomal proteolysis mechanism, with three Keio strains; the data showed a correlation between IPTG concentration and amount of protein degradation. Overall, Lambert iGEM desired to promote scientific research under any financial circumstance and to characterize non-lysosomal inducible protein degradation (CLiP’d). |
Revision as of 10:40, 30 October 2017
Description
![](https://static.igem.org/mediawiki/2017/b/bc/T--Lambert_GA--purpleline.png)
To conduct synthetic biology research, underfunded labs often lack the monetary means of buying expensive laboratory equipment, such as a plate reader to measure fluorescence of cells or a centrifuge to pellet cells. These challenges are often insurmountable for small research facilities or scientists in the field. The approach Lambert iGEM used was multifaceted; design a chamber to optimize and standardize conditions for detecting color, write a software application that can be used by anyone to quantify the color values using HSL, test and improve on a low cost centrifuge, assemble and test these devices with chromoproteins under varying levels of IPTG induction.
Using engineering design principles, the 2017 Lambert iGEM team aimed to develop an imaging device called the Chrome-Q to cost-effectively standardize conditions for quantifying chromoprotein data; additionally, the team improved upon the Paperfuge originally designed by the Prakash Lab to pellet cells at a low cost. Different chromoproteins - Tinsel Purple, Virginia Violet and Scrooge Orange - were induced at varying levels of IPTG, causing the cells to express different intensities; pictures of the cells were taken using a Chrome-Q and were measured with a self-constructed software app that analyzes the pigments using HSV color space. Additionally, the team observed that a common issue encountered by synthetic biologists is the overlap of protein expression in development of genetic circuits which negatively affects the quality of performance in a given cell. The 2017 Lambert iGEM Team worked to develop an inducible “switch” to further characterize ClpXP, a non-lysosomal proteolysis mechanism, with three Keio strains; the data showed a correlation between IPTG concentration and amount of protein degradation. Overall, Lambert iGEM desired to promote scientific research under any financial circumstance and to characterize non-lysosomal inducible protein degradation (CLiP’d).![](https://static.igem.org/mediawiki/2017/b/b3/T--Lambert_GA--overexpression.png)
![](https://static.igem.org/mediawiki/2017/5/5d/T--Lambert_GA--system.png)
![](https://static.igem.org/mediawiki/2017/c/cc/T--Lambert_GA--SSRApathway.png)
![](https://static.igem.org/mediawiki/2017/e/e0/T--Lambert_GA--clpxpanimation.gif)
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