Difference between revisions of "Team:Munich/LabJournal"
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<li> | <li> | ||
| − | Cells had OD of 5.97 -> 4.78*10 | + | Cells had OD of 5.97 -> 4.78*10<sup>9</sup> cells according to http://www.genomics.agilent.com/biocalculators/calcODBacterial.jsp?_requestid=1029158 |
</li> | </li> | ||
<li> | <li> | ||
| − | Cells had OD of 5.97 -> 4.78*10 | + | Cells had OD of 5.97 -> 4.78*10<sup>9</sup> cells according to http://www.genomics.agilent.com/biocalculators/calcODBacterial.jsp?_requestid=1029158 </br> |
| − | -> dilluted 1:100 (to V=10mL) and pelleted 209 miL to get to 10 | + | -> dilluted 1:100 (to V=10mL) and pelleted 209 miL to get to 10<sup>7</sup> cells |
</li> | </li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | + | <table class="mediocre-tableEditable-table u-small-spacing-right" style="top: 0px; width: 968px;"><colgroup><col style="width: 45px;"><col data-id="0" style="width: 250px;"><col data-id="1" style="width: 232px;"><col data-id="2" style="width: 216px;"><col data-id="3" style="width: 225px;"></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">D</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-0-0" data-namespace="table-ns3555"><span>Method</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-0-1" data-namespace="table-ns3555"><span>SDS</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-0-2" data-namespace="table-ns3555"><span>NaOH</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-0-3" data-namespace="table-ns3555"><span>TRIzol</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-1-0" data-namespace="table-ns3555"><span>prepare</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-1-1" data-namespace="table-ns3555"><span>1 mL TES (10mM Tris-HCl, 10mM EDTA, pH 8.0, 2% SDS -> TE-Buffer + SDS...</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-1-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-1-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-2-0" data-namespace="table-ns3555"><span>----Lysis</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-2-1" data-namespace="table-ns3555"><span>1x</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-2-2" data-namespace="table-ns3555"><span>3x</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-2-3" data-namespace="table-ns3555"><span>1x</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-3-0" data-namespace="table-ns3555"><span>separate to tubes Spin 300xG, 5 min ->Ice, TROzol to -20! (100 miL at OD 1 -> 10<sup>7</sup> cells, Max. according to TRIzolPaper</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-3-1" data-namespace="table-ns3555"><span>100 miL bactSusp</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-3-2" data-namespace="table-ns3555"><span>100 miL bact-susp</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-3-3" data-namespace="table-ns3555"><span>100 miL bact-suspension</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-4-0" data-namespace="table-ns3555"><span>No washing step, bad for mRNA (TRIzolP.)</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-4-1" data-namespace="table-ns3555"><span> 300 µl (0.45 ml) TES resuspend pellet</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-4-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-4-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-5-0" data-namespace="table-ns3555"><span>12,5 uL Inhibitor (40k U/ml, 1 U/miL required)</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-5-1" data-namespace="table-ns3555"><span>15 min, 75 °C lysis, vortex</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-5-2" data-namespace="table-ns3555"><span>one probe after another</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-5-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-6-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-6-1" data-namespace="table-ns3555"><span>(Addition of inhibitor); Transform total volume to gel tube</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-6-2" data-namespace="table-ns3555"><span>resuspend thorougly in 66 µl (100 miL) Solution1</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-6-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-7-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-7-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-7-2" data-namespace="table-ns3555"><span>132 µl (200 miL) Sol2, INVERT 4x</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-7-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">9</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-8-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-8-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-8-2" data-namespace="table-ns3555"><span>wait 0, 1 ,3 min</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-8-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">10</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-9-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-9-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-9-2" data-namespace="table-ns3555"><span>add 99 µl (150 miL) Sol3, INVERT 4x</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-9-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">11</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-10-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-10-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-10-2" data-namespace="table-ns3555"><span>Total volume: 297 µl (0.45 ml)</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-10-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">12</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-11-0" data-namespace="table-ns3555"><span>--phenChloExtr</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-11-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-11-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-11-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">13</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-12-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" rowspan="1" colspan="2" data-cid="table-note-part2402-12-1" data-namespace="table-ns3555"><span>measure pH, add acid...</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-12-3" data-namespace="table-ns3555"><span>meassure Trizol PH, add acid if needed (pH <5)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">14</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-13-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" rowspan="1" colspan="2" data-cid="table-note-part2402-13-1" data-namespace="table-ns3555"><span>add 600 µl [same V (0.45 ml)] Phe:chloro.</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-13-3" data-namespace="table-ns3555"><span>add 1 mL TRIzol to 10<sup>7</sup> cells</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">15</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-14-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" rowspan="1" colspan="2" data-cid="table-note-part2402-14-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-14-3" data-namespace="table-ns3555"><span>5 min incubation</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">16</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-15-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" rowspan="1" colspan="2" data-cid="table-note-part2402-15-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-15-3" data-namespace="table-ns3555"><span>add 0.2 mL CHLOROPHORM</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">17</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-16-0" data-namespace="table-ns3555" style="color: rgb(52, 64, 67);"><span>incub 2.5 (2-3) min, RT</span></td><td class="cell is-text is-alignLeft cell-contents" rowspan="1" colspan="2" data-cid="table-note-part2402-16-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-16-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">18</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-17-0" data-namespace="table-ns3555" style="color: rgb(52, 64, 67);"><span>centri 12k g 15 min 4°C</span></td><td class="cell is-text is-alignLeft cell-contents" rowspan="1" colspan="2" data-cid="table-note-part2402-17-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-17-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">19</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-18-0" data-namespace="table-ns3555"><span>angle 45°, extract top phase carefully</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-18-1" data-namespace="table-ns3555"><span>-> discard everything else, prot & DNA</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-18-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-18-3" data-namespace="table-ns3555"><span>-> which Vol for the TRIzol aqueous phase?</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">20</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-19-0" data-namespace="table-ns3555"><span>add 0.4 (0.375) ml Isopropanol, wait 10 min, RT</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-19-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-19-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-19-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">21</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-20-0" data-namespace="table-ns3555"><span>centrifuge 10 min 12k , 4°C -> </span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-20-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-20-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-20-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">22</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-21-0" data-namespace="table-ns3555" style="color: rgb(225, 69, 22);"><span>Aditional step, because RNA pellet was not entierly spinned down: Centrifugation (16000 rcf, 5 min, 4 °C)</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-21-1" data-namespace="table-ns3555"><span>big-pellet assay in fridge, but 1. interphase was punctured/gel(DNA?) stuck to pipette 2. s.u.</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-21-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-21-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">23</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-22-0" data-namespace="table-ns3555" style="color: rgb(225, 69, 22);"><span>Aditional step, because suspected RNA fog was not entirely spinned down: remove upper and lower RNA fog phase</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-22-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-22-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-22-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">24</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-23-0" data-namespace="table-ns3555" style="color: rgb(225, 69, 22);"><span>Additional step: Centrifugation (16000 rcf, 5 min, 4 °C)</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-23-1" data-namespace="table-ns3555" style="color: rgb(225, 69, 22);"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-23-2" data-namespace="table-ns3555" style="color: rgb(225, 69, 22);"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-23-3" data-namespace="table-ns3555" style="color: rgb(225, 69, 22);"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">25</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-24-0" data-namespace="table-ns3555" style="color: rgb(225, 69, 22);"><span>No RNA pellet or RNA fog any more :(</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-24-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-24-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-24-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">26</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-25-0" data-namespace="table-ns3555" style="color: rgb(225, 69, 22);"><span>Addition of 400 µl isopropanol -> No RNA pellet or fog any more ->prob. removed too much</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-25-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-25-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-25-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">27</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-26-0" data-namespace="table-ns3555"><span>discard supernatant -> RNA in gel-like pellet!</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-26-1" data-namespace="table-ns3555"><span>-> gel hardly visible, nearly draged it out of tube (stuck to pipette...)</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-26-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-26-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">28</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-27-0" data-namespace="table-ns3555" style="color: rgb(0, 0, 0);"><span>resuspend in 0.75 ml 70 % (75% ideally) ethanol</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-27-1" data-namespace="table-ns3555"><span>-> storeed RNA at -20°C is stable up to 1 year...</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-27-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-27-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">29</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-28-0" data-namespace="table-ns3555"><span>vortex, centri 5 min 7,5k 4°</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-28-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-28-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-28-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">30</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-29-0" data-namespace="table-ns3555"><span>discard supernatant, air dry for 5-10 min (do NOT let completely dry out)</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-29-1" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-29-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-29-3" data-namespace="table-ns3555"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">31</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-30-0" data-namespace="table-ns3555"><span>resuspend in RNAse free water 30 µl (20-50 miL) </span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-30-1" data-namespace="table-ns3555"><span>->measurement...</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-30-2" data-namespace="table-ns3555"><span></span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2402-30-3" data-namespace="table-ns3555"><span></span></td></tr></tbody></table> | |
<li class="myResults"> | <li class="myResults"> | ||
<h4>Results</h4> | <h4>Results</h4> | ||
| Line 1,747: | Line 1,747: | ||
<ul> | <ul> | ||
<li> | <li> | ||
| − | Cell suspension had OD600 (extrapolated) of 2,220 -> 1.78 x 10 | + | Cell suspension had OD600 (extrapolated) of 2,220 -> 1.78 x 10<sup>9</sup> cells/ml according to http://www.genomics.agilent.com/biocalculators/calcODBacterial.jsp?_requestid=1029158 |
</li> | </li> | ||
<li> | <li> | ||
| − | Experiments were done with 108 and 109 cells per sample, because it failed with 10 | + | Experiments were done with 108 and 109 cells per sample, because it failed with 10<sup>7</sup> cells last time |
</li> | </li> | ||
<li> | <li> | ||
| Line 1,757: | Line 1,757: | ||
<li> | <li> | ||
No pellet after RNA precipitation (-80 °C) and subsequent centrifugation in: | No pellet after RNA precipitation (-80 °C) and subsequent centrifugation in: | ||
| − | <ul> <li>NaOH, 1 min, 10 | + | <ul> <li>NaOH, 1 min, 10<sup>8</sup></li> |
| − | <li>SDS, 10 | + | <li>SDS, 10<sup>9</sup></li> |
| − | <li>Trizol, 10 | + | <li>Trizol, 10<sup>8</sup></li></ul> |
</li> | </li> | ||
<li> Mistakes: | <li> Mistakes: | ||
| Line 1,765: | Line 1,765: | ||
<ul> | <ul> | ||
| − | <li>SDS, 10 | + | <li>SDS, 10<sup>9</sup>: aspirated 100 µl instead of 200 µl supernatant</li> |
| − | <li>NaOH, 10 | + | <li>NaOH, 10<sup>8</sup>, 3 min: contamination with DNA (<10% DNA interphase transfered) </li> |
| − | <li>NaOH, 10 | + | <li>NaOH, 10<sup>9</sup>, 3 min: added + 200 µl of SDS 109 tube</li></ul> |
<li>! Did not calibrate alkaline lysis solution 3 to given pH -> probably more RNA degradation</li> | <li>! Did not calibrate alkaline lysis solution 3 to given pH -> probably more RNA degradation</li> | ||
<li>! Did store RNA at -20 °C instead of -80 -> probably more RNA degradation</li> | <li>! Did store RNA at -20 °C instead of -80 -> probably more RNA degradation</li> | ||
| Line 1,775: | Line 1,775: | ||
<li class="myResults"> | <li class="myResults"> | ||
<h4>Results</h4> | <h4>Results</h4> | ||
| − | + | <table class="mediocre-tableEditable-table u-small-spacing-right" style="top: 0px; width: 988px;"><colgroup><col style="width: 45px;"><col data-id="0" style="width: 143px;"><col data-id="1" style="width: 100px;"><col data-id="2" style="width: 100px;"><col data-id="3" style="width: 100px;"><col data-id="4" style="width: 100px;"><col data-id="5" style="width: 100px;"><col data-id="6" style="width: 100px;"><col data-id="7" style="width: 100px;"><col data-id="8" style="width: 100px;"></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">Sample</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">[] [µg/ml]</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">adjusted C**</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A260/A280</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A260/A230</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A230 [A]</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A260 [A]</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A280 [A]</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A320 [A]</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2455-0-0" data-namespace="table-ns3555"><span>SDS, 10<sup>8</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-0-1" data-namespace="table-ns3555"><span>36.8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-0-2" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(246, 217, 77);"><span>55</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-0-3" data-namespace="table-ns3555"><span>1.704</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-0-4" data-namespace="table-ns3555"><span>2.044</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-0-5" data-namespace="table-ns3555"><span>0.047</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-0-6" data-namespace="table-ns3555"><span>0.094</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-0-7" data-namespace="table-ns3555"><span>0.056</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-0-8" data-namespace="table-ns3555"><span>0.002</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2455-1-0" data-namespace="table-ns3555"><span>SDS, 10<sup>9</sup> cells*</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-1-1" data-namespace="table-ns3555"><span>119</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-1-2" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(246, 217, 77);"><span>357</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-1-3" data-namespace="table-ns3555"><span>1.693</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-1-4" data-namespace="table-ns3555"><span>2.069</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-1-5" data-namespace="table-ns3555"><span>0.146</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-1-6" data-namespace="table-ns3555"><span>0.300</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-1-7" data-namespace="table-ns3555"><span>0.178</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-1-8" data-namespace="table-ns3555"><span>0.002</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2455-2-0" data-namespace="table-ns3555"><span>NaOH, 0 min, 10<sup>8</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-2-1" data-namespace="table-ns3555"><span>35.2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-2-2" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(246, 217, 77);"><span>53</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-2-3" data-namespace="table-ns3555"><span>1.725</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-2-4" data-namespace="table-ns3555"><span>2.146</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-2-5" data-namespace="table-ns3555"><span>0.042</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-2-6" data-namespace="table-ns3555"><span>0.089</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-2-7" data-namespace="table-ns3555"><span>0.052</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-2-8" data-namespace="table-ns3555"><span>0.001</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2455-3-0" data-namespace="table-ns3555"><span>NaOH, 0 min, 10<sup>9</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-3-1" data-namespace="table-ns3555"><span>586</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-3-2" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(246, 217, 77);"><span>879</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-3-3" data-namespace="table-ns3555" style="background-color: rgb(132, 198, 32);"><span>1.923</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-3-4" data-namespace="table-ns3555"><span>1.993</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-3-5" data-namespace="table-ns3555"><span>0.740</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-3-6" data-namespace="table-ns3555"><span>1.470</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-3-7" data-namespace="table-ns3555"><span>0.767</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-3-8" data-namespace="table-ns3555"><span>0.005</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2455-4-0" data-namespace="table-ns3555"><span>NaOH, 1 min, 10^8 cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-4-1" data-namespace="table-ns3555"><span>73.6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-4-2" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(246, 217, 77);"><span>110</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-4-3" data-namespace="table-ns3555"><span>1.235</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-4-4" data-namespace="table-ns3555"><span>0.586</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-4-5" data-namespace="table-ns3555"><span>0.317</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-4-6" data-namespace="table-ns3555"><span>0.187</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-4-7" data-namespace="table-ns3555"><span>0.152</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-4-8" data-namespace="table-ns3555"><span>0.003</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2455-5-0" data-namespace="table-ns3555"><span>NaOH, 1 min, 10<sup>9</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-5-1" data-namespace="table-ns3555"><span>370</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-5-2" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(246, 217, 77);"><span>555</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-5-3" data-namespace="table-ns3555" style="background-color: rgb(132, 198, 32);"><span>1.945</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-5-4" data-namespace="table-ns3555"><span>1.958</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-5-5" data-namespace="table-ns3555"><span>0.475</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-5-6" data-namespace="table-ns3555"><span>0.927</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-5-7" data-namespace="table-ns3555"><span>0.478</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-5-8" data-namespace="table-ns3555"><span>0.003</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2455-6-0" data-namespace="table-ns3555"><span>NaOH, 3 min, 10<sup>8</sup> cells*</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-6-1" data-namespace="table-ns3555"><span>259</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-6-2" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(246, 217, 77);"><span>389</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-6-3" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(166, 212, 97);"><span>1.849</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-6-4" data-namespace="table-ns3555"><span>1.491</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-6-5" data-namespace="table-ns3555"><span>0.439</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-6-6" data-namespace="table-ns3555"><span>0.652</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-6-7" data-namespace="table-ns3555"><span>0.355</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-6-8" data-namespace="table-ns3555"><span>0.005</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2455-7-0" data-namespace="table-ns3555"><span>NaOH, 3 min, 10<sup>9</sup> cells*</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-7-1" data-namespace="table-ns3555"><span>275</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-7-2" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(246, 217, 77);"><span>413</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-7-3" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(166, 212, 97);"><span>1.850</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-7-4" data-namespace="table-ns3555"><span>2.212</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-7-5" data-namespace="table-ns3555"><span>0.316</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-7-6" data-namespace="table-ns3555"><span>0.693</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-7-7" data-namespace="table-ns3555"><span>0.368</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-7-8" data-namespace="table-ns3555"><span>0.005</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">9</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2455-8-0" data-namespace="table-ns3555"><span>TRIzol, 10<sup>8</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-8-1" data-namespace="table-ns3555"><span>194</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-8-2" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(246, 217, 77);"><span>265</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-8-3" data-namespace="table-ns3555"><span>1.362</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-8-4" data-namespace="table-ns3555"><span>0.221</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-8-5" data-namespace="table-ns3555"><span>2.200</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-8-6" data-namespace="table-ns3555"><span>0.487</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-8-7" data-namespace="table-ns3555"><span>0.358</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-8-8" data-namespace="table-ns3555"><span>0.002</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">10</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2455-9-0" data-namespace="table-ns3555"><span>TRIzol, 10<sup>9</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-9-1" data-namespace="table-ns3555"><span>160</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-9-2" data-namespace="table-ns3555" style="color: rgb(0, 0, 0); background-color: rgb(246, 217, 77);"><span>218</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-9-3" data-namespace="table-ns3555"><span>1.429</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-9-4" data-namespace="table-ns3555"><span>0.488</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-9-5" data-namespace="table-ns3555"><span>0.822</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-9-6" data-namespace="table-ns3555"><span>0.403</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-9-7" data-namespace="table-ns3555"><span>0.283</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2455-9-8" data-namespace="table-ns3555"><span>0.003</span></td></tr></tbody></table> | |
</li><li>Measured [RNA] by Implen Nanophotometer (20170616).</li> | </li><li>Measured [RNA] by Implen Nanophotometer (20170616).</li> | ||
<li>**adjusted for different supernatant V aspirated at extraction step</li> | <li>**adjusted for different supernatant V aspirated at extraction step</li> | ||
| Line 2,241: | Line 2,241: | ||
</li> | </li> | ||
<li> | <li> | ||
| − | + | <table class="mediocre-tableEditable-table u-small-spacing-right" style="top: 0px; width: 1031px;"><colgroup><col style="width: 45px;"><col data-id="0" style="width: 143px;"><col data-id="1" style="width: 143px;"><col data-id="2" style="width: 100px;"><col data-id="3" style="width: 100px;"><col data-id="4" style="width: 100px;"><col data-id="5" style="width: 100px;"><col data-id="6" style="width: 100px;"><col data-id="7" style="width: 100px;"><col data-id="8" style="width: 100px;"></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">Sample</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">Number of sample</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">[] [µg/ml]</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A260/A280</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A260/A230</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A230 [A]</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A260 [A]</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A280 [A]</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div><span class="mediocre-tableEditable-axisCell-num">A320 [A]</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-0-0" data-namespace="table-ns3555"><span>SDS, 10<sup>8</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-0-1" data-namespace="table-ns3555"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-0-2" data-namespace="table-ns3555"><span>62.4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-0-3" data-namespace="table-ns3555"><span>2.080</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-0-4" data-namespace="table-ns3555"><span>0.118</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-0-5" data-namespace="table-ns3555"><span>1.343</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-0-6" data-namespace="table-ns3555"><span>0.174</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-0-7" data-namespace="table-ns3555"><span>0.093</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-0-8" data-namespace="table-ns3555"><span>0.018</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-1-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-1-1" data-namespace="table-ns3555"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-1-2" data-namespace="table-ns3555"><span>56.8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-1-3" data-namespace="table-ns3555"><span>2.029</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-1-4" data-namespace="table-ns3555"><span>0.128</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-1-5" data-namespace="table-ns3555"><span>1.123</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-1-6" data-namespace="table-ns3555"><span>0.153</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-1-7" data-namespace="table-ns3555"><span>0.081</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-1-8" data-namespace="table-ns3555"><span>0.011</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-2-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-2-1" data-namespace="table-ns3555"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-2-2" data-namespace="table-ns3555"><span>47.6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-2-3" data-namespace="table-ns3555"><span>1.983</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-2-4" data-namespace="table-ns3555"><span>0.102</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-2-5" data-namespace="table-ns3555"><span>1.178</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-2-6" data-namespace="table-ns3555"><span>0.127</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-2-7" data-namespace="table-ns3555"><span>0.068</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-2-8" data-namespace="table-ns3555"><span>0.008</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-3-0" data-namespace="table-ns3555"><span>SDS, 10<sup>9</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-3-1" data-namespace="table-ns3555"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-3-2" data-namespace="table-ns3555"><span>354</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-3-3" data-namespace="table-ns3555"><span>1.782</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-3-4" data-namespace="table-ns3555"><span>>0.4</span></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-3-5" data-namespace="table-ns3555"><span>>2.5</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-3-6" data-namespace="table-ns3555"><span>0.913</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-3-7" data-namespace="table-ns3555"><span>0.525</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-3-8" data-namespace="table-ns3555"><span>0.029</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-4-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-4-1" data-namespace="table-ns3555"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-4-2" data-namespace="table-ns3555"><span>260</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-4-3" data-namespace="table-ns3555"><span>1.857</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-4-4" data-namespace="table-ns3555"><span>0.295</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-4-5" data-namespace="table-ns3555"><span>2.222</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-4-6" data-namespace="table-ns3555"><span>0.665</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-4-7" data-namespace="table-ns3555"><span>0.365</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-4-8" data-namespace="table-ns3555"><span>0.015</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-5-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-5-1" data-namespace="table-ns3555"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-5-2" data-namespace="table-ns3555"><span>376</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-5-3" data-namespace="table-ns3555"><span>1.801</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-5-4" data-namespace="table-ns3555"><span>0.392</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-5-5" data-namespace="table-ns3555"><span>2.422</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-5-6" data-namespace="table-ns3555"><span>0.962</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-5-7" data-namespace="table-ns3555"><span>0.544</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-5-8" data-namespace="table-ns3555"><span>0.022</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-6-0" data-namespace="table-ns3555"><span>NaOH, 0 min, 10<sup>8</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-6-1" data-namespace="table-ns3555"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-6-2" data-namespace="table-ns3555"><span>39.2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-6-3" data-namespace="table-ns3555" style="color: rgb(0, 0, 0);"><span>1.690</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-6-4" data-namespace="table-ns3555"><span>2.042</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-6-5" data-namespace="table-ns3555"><span>0.056</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-6-6" data-namespace="table-ns3555"><span>0.106</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-6-7" data-namespace="table-ns3555"><span>0.066</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-6-8" data-namespace="table-ns3555"><span>0.008</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-7-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-7-1" data-namespace="table-ns3555"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-7-2" data-namespace="table-ns3555"><span>53.6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-7-3" data-namespace="table-ns3555" style="color: rgb(0, 0, 0);"><span>1.614</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-7-4" data-namespace="table-ns3555"><span>1.887</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-7-5" data-namespace="table-ns3555"><span>0.086</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-7-6" data-namespace="table-ns3555"><span>0.149</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-7-7" data-namespace="table-ns3555"><span>0.098</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-7-8" data-namespace="table-ns3555"><span>0.015</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">9</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-8-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-8-1" data-namespace="table-ns3555"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-8-2" data-namespace="table-ns3555"><span>68.8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-8-3" data-namespace="table-ns3555"><span>1.720</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-8-4" data-namespace="table-ns3555"><span>2.457</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-8-5" data-namespace="table-ns3555"><span>0.070</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-8-6" data-namespace="table-ns3555"><span>0.172</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-8-7" data-namespace="table-ns3555"><span>0.100</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-8-8" data-namespace="table-ns3555"><span>0.000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">10</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-9-0" data-namespace="table-ns3555"><span>NaOH, 0 min, 10<sup>9</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-9-1" data-namespace="table-ns3555"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-9-2" data-namespace="table-ns3555"><span>629</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-9-3" data-namespace="table-ns3555"><span>1.911</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-9-4" data-namespace="table-ns3555"><span>2.466</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-9-5" data-namespace="table-ns3555"><span>0.643</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-9-6" data-namespace="table-ns3555"><span>1.578</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-9-7" data-namespace="table-ns3555"><span>0.828</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-9-8" data-namespace="table-ns3555"><span>0.005</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">11</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-10-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-10-1" data-namespace="table-ns3555"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-10-2" data-namespace="table-ns3555" style="background-color: rgb(249, 220, 220);"><span>17.6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-10-3" data-namespace="table-ns3555"><span>1.692</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-10-4" data-namespace="table-ns3555"><span>2.200</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-10-5" data-namespace="table-ns3555"><span>0.019</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-10-6" data-namespace="table-ns3555"><span>0.043</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-10-7" data-namespace="table-ns3555"><span>0.025</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-10-8" data-namespace="table-ns3555"><span>-0.001</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">12</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-11-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-11-1" data-namespace="table-ns3555"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-11-2" data-namespace="table-ns3555"><span>619</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-11-3" data-namespace="table-ns3555"><span>1.897</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-11-4" data-namespace="table-ns3555"><span>2.453</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-11-5" data-namespace="table-ns3555"><span>0.636</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-11-6" data-namespace="table-ns3555"><span>1.553</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-11-7" data-namespace="table-ns3555"><span>0.821</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-11-8" data-namespace="table-ns3555"><span>0.005</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">13</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-12-0" data-namespace="table-ns3555"><span>NaOH, 1 min, 10<sup>8</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-12-1" data-namespace="table-ns3555"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-12-2" data-namespace="table-ns3555"><span>52</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-12-3" data-namespace="table-ns3555"><span>1.806</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-12-4" data-namespace="table-ns3555"><span>2.203</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-12-5" data-namespace="table-ns3555"><span>0.059</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-12-6" data-namespace="table-ns3555"><span>0.130</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-12-7" data-namespace="table-ns3555"><span>0.072</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-12-8" data-namespace="table-ns3555"><span>0.000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">14</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-13-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-13-1" data-namespace="table-ns3555"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-13-2" data-namespace="table-ns3555"><span>33.2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-13-3" data-namespace="table-ns3555"><span>1.729</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-13-4" data-namespace="table-ns3555"><span>2.371</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-13-5" data-namespace="table-ns3555"><span>0.035</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-13-6" data-namespace="table-ns3555"><span>0.083</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-13-7" data-namespace="table-ns3555"><span>0.048</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-13-8" data-namespace="table-ns3555"><span>0.000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">15</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-14-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-14-1" data-namespace="table-ns3555"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-14-2" data-namespace="table-ns3555"><span>58.4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-14-3" data-namespace="table-ns3555"><span>1.738</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-14-4" data-namespace="table-ns3555"><span>2.393</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-14-5" data-namespace="table-ns3555"><span>0.061</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-14-6" data-namespace="table-ns3555"><span>0.146</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-14-7" data-namespace="table-ns3555"><span>0.084</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-14-8" data-namespace="table-ns3555"><span>0.000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">16</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-15-0" data-namespace="table-ns3555"><span>NaOH, 1 min, 10<sup>9</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-15-1" data-namespace="table-ns3555"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-15-2" data-namespace="table-ns3555"><span>606</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-15-3" data-namespace="table-ns3555"><span>1.893</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-15-4" data-namespace="table-ns3555"><span>2.469</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-15-5" data-namespace="table-ns3555"><span>0.620</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-15-6" data-namespace="table-ns3555"><span>1.522</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-15-7" data-namespace="table-ns3555"><span>0.807</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-15-8" data-namespace="table-ns3555"><span>0.006</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">17</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-16-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-16-1" data-namespace="table-ns3555"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-16-2" data-namespace="table-ns3555"><span>627</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-16-3" data-namespace="table-ns3555"><span>1.898</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-16-4" data-namespace="table-ns3555"><span>2.431</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-16-5" data-namespace="table-ns3555"><span>0.651</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-16-6" data-namespace="table-ns3555"><span>1.574</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-16-7" data-namespace="table-ns3555"><span>0.832</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-16-8" data-namespace="table-ns3555"><span>0.006</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">18</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-17-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-17-1" data-namespace="table-ns3555"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-17-2" data-namespace="table-ns3555" style="background-color: rgb(249, 220, 220);"><span>22.4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-17-3" data-namespace="table-ns3555"><span>1.806</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-17-4" data-namespace="table-ns3555"><span>2.435</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-17-5" data-namespace="table-ns3555"><span>0.023</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-17-6" data-namespace="table-ns3555"><span>0.056</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-17-7" data-namespace="table-ns3555"><span>0.031</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-17-8" data-namespace="table-ns3555"><span>0.000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">19</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-18-0" data-namespace="table-ns3555"><span>NaOH, 3 min, 10<sup>8</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-18-1" data-namespace="table-ns3555"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-18-2" data-namespace="table-ns3555"><span>44</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-18-3" data-namespace="table-ns3555"><span>1.864</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-18-4" data-namespace="table-ns3555"><span>2.444</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-18-5" data-namespace="table-ns3555"><span>0.044</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-18-6" data-namespace="table-ns3555"><span>0.109</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-18-7" data-namespace="table-ns3555"><span>0.058</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-18-8" data-namespace="table-ns3555"><span>-0.001</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">20</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-19-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-19-1" data-namespace="table-ns3555"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-19-2" data-namespace="table-ns3555"><span>61.2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-19-3" data-namespace="table-ns3555"><span>1.779</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-19-4" data-namespace="table-ns3555"><span>2.593</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-19-5" data-namespace="table-ns3555"><span>0.058</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-19-6" data-namespace="table-ns3555"><span>0.152</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-19-7" data-namespace="table-ns3555"><span>0.085</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-19-8" data-namespace="table-ns3555"><span>-0.001</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">21</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-20-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-20-1" data-namespace="table-ns3555"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-20-2" data-namespace="table-ns3555" style="background-color: rgb(249, 220, 220);"><span>5.6</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-20-3" data-namespace="table-ns3555"><span>1.400</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-20-4" data-namespace="table-ns3555"><span>2.800</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-20-5" data-namespace="table-ns3555"><span>0.004</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-20-6" data-namespace="table-ns3555"><span>0.013</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-20-7" data-namespace="table-ns3555"><span>0.009</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-20-8" data-namespace="table-ns3555"><span>-0.001</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">22</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-21-0" data-namespace="table-ns3555"><span>NaOH, 3 min, 10<sup>9</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-21-1" data-namespace="table-ns3555"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-21-2" data-namespace="table-ns3555"><span>619</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-21-3" data-namespace="table-ns3555"><span>1.908</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-21-4" data-namespace="table-ns3555"><span>2.432</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-21-5" data-namespace="table-ns3555"><span>0.643</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-21-6" data-namespace="table-ns3555"><span>1.554</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-21-7" data-namespace="table-ns3555"><span>0.818</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-21-8" data-namespace="table-ns3555"><span>0.007</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">23</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-22-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-22-1" data-namespace="table-ns3555"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-22-2" data-namespace="table-ns3555"><span>570</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-22-3" data-namespace="table-ns3555"><span>1.927</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-22-4" data-namespace="table-ns3555"><span>2.468</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-22-5" data-namespace="table-ns3555"><span>0.583</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-22-6" data-namespace="table-ns3555"><span>1.430</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-22-7" data-namespace="table-ns3555"><span>0.745</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-22-8" data-namespace="table-ns3555"><span>0.006</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">24</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-23-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-23-1" data-namespace="table-ns3555"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-23-2" data-namespace="table-ns3555" style="background-color: rgb(249, 220, 220);"><span>20.4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-23-3" data-namespace="table-ns3555"><span>1.759</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-23-4" data-namespace="table-ns3555"><span>2.429</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-23-5" data-namespace="table-ns3555"><span>0.020</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-23-6" data-namespace="table-ns3555"><span>0.050</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-23-7" data-namespace="table-ns3555"><span>0.028</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-23-8" data-namespace="table-ns3555"><span>-0.001</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">25</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-24-0" data-namespace="table-ns3555"><span>NaOH, 10 min, 10<sup>8</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-24-1" data-namespace="table-ns3555"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-24-2" data-namespace="table-ns3555"><span>10.8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-24-3" data-namespace="table-ns3555"><span>1.588</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-24-4" data-namespace="table-ns3555"><span>2.250</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-24-5" data-namespace="table-ns3555"><span>0.012</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-24-6" data-namespace="table-ns3555"><span>0.027</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-24-7" data-namespace="table-ns3555"><span>0.017</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-24-8" data-namespace="table-ns3555"><span>0.000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">26</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-25-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-25-1" data-namespace="table-ns3555"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-25-2" data-namespace="table-ns3555"><span>8.8</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-25-3" data-namespace="table-ns3555"><span>1.571</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-25-4" data-namespace="table-ns3555"><span>3.143</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-25-5" data-namespace="table-ns3555"><span>0.006</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-25-6" data-namespace="table-ns3555"><span>0.021</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-25-7" data-namespace="table-ns3555"><span>0.013</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-25-8" data-namespace="table-ns3555"><span>-0.001</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">27</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-26-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-26-1" data-namespace="table-ns3555"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-26-2" data-namespace="table-ns3555"><span>4.4</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-26-3" data-namespace="table-ns3555"><span>1.375</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-26-4" data-namespace="table-ns3555"><span>3.667</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-26-5" data-namespace="table-ns3555"><span>0.002</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-26-6" data-namespace="table-ns3555"><span>0.010</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-26-7" data-namespace="table-ns3555"><span>0.007</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-26-8" data-namespace="table-ns3555"><span>-0.001</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">28</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-27-0" data-namespace="table-ns3555"><span>NaOH, 10 min, 10<sup>9</sup> cells</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-27-1" data-namespace="table-ns3555"><span>1</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-27-2" data-namespace="table-ns3555"><span>503</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-27-3" data-namespace="table-ns3555"><span>1.916</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-27-4" data-namespace="table-ns3555"><span>2.499</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-27-5" data-namespace="table-ns3555"><span>0.508</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-27-6" data-namespace="table-ns3555"><span>1.262</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-27-7" data-namespace="table-ns3555"><span>0.661</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-27-8" data-namespace="table-ns3555"><span>0.005</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">29</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-28-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-28-1" data-namespace="table-ns3555"><span>2</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-28-2" data-namespace="table-ns3555"><span>564</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-28-3" data-namespace="table-ns3555"><span>1.911</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-28-4" data-namespace="table-ns3555"><span>2.487</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-28-5" data-namespace="table-ns3555"><span>0.572</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-28-6" data-namespace="table-ns3555"><span>1.415</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-28-7" data-namespace="table-ns3555"><span>0.743</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-28-8" data-namespace="table-ns3555"><span>0.005</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div><span class="mediocre-tableEditable-axisCell-num">30</span></div></td><td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part2640-29-0" data-namespace="table-ns3555"><span></span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-29-1" data-namespace="table-ns3555"><span>3</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-29-2" data-namespace="table-ns3555"><span>611</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-29-3" data-namespace="table-ns3555"><span>1.902</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-29-4" data-namespace="table-ns3555"><span>2.467</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-29-5" data-namespace="table-ns3555"><span>0.625</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-29-6" data-namespace="table-ns3555"><span>1.533</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-29-7" data-namespace="table-ns3555"><span>0.809</span></td><td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part2640-29-8" data-namespace="table-ns3555"><span>0.006</span></td></tr></tbody></table> | |
</li> | </li> | ||
</ul> | </ul> | ||
| Line 2,607: | Line 2,607: | ||
</li> | </li> | ||
<li> | <li> | ||
| − | Specification: Use in range of 10 | + | Specification: Use in range of 10<sup>2</sup> to 10<sup>6</sup> cells. Tested 10<sup>6</sup> and 10<sup>8</sup> cells |
</li> | </li> | ||
</ul> | </ul> | ||
| Line 2,613: | Line 2,613: | ||
<li class="myResults"> | <li class="myResults"> | ||
<h4>Results</h4> | <h4>Results</h4> | ||
| − | No nanodrop-measureable amount of RNA/DNA with 10 | + | No nanodrop-measureable amount of RNA/DNA with 10<sup>6</sup> cell, <10 ug/ml RNA with 10<sup>8</sup> cells |
Terrible yield, repeat again to be sure | Terrible yield, repeat again to be sure | ||
</li> | </li> | ||
| Line 2,652: | Line 2,652: | ||
</li> | </li> | ||
<li> | <li> | ||
| − | Specification: Use in range of 10 | + | Specification: Use in range of 10<sup>2</sup> to 10<sup>6</sup> cells. Tested 10<sup>6</sup> and 10<sup>8</sup> cells |
</li> | </li> | ||
</ul> | </ul> | ||
| Line 2,658: | Line 2,658: | ||
<li class="myResults"> | <li class="myResults"> | ||
<h4>Results</h4> | <h4>Results</h4> | ||
| − | Again no measureable amount of RNA for 10 | + | Again no measureable amount of RNA for 10<sup>6</sup> cells. |
| − | For 10 | + | For 10<sup>8</sup> cells, RNA concentration was increased through column-binding and elution (small elution volume) |
Elution seems effective, second elution has only 20 % of first elution's amount of RNA | Elution seems effective, second elution has only 20 % of first elution's amount of RNA | ||
</li> | </li> | ||
| Line 4,310: | Line 4,310: | ||
<ul> | <ul> | ||
<li> | <li> | ||
| − | Diluted E. coli culture to 10 | + | Diluted E. coli culture to 10<sup>8</sup> cells/ml, used 300 uL as sample |
</li> | </li> | ||
<li> | <li> | ||
| Line 4,320: | Line 4,320: | ||
<h4>Results</h4> | <h4>Results</h4> | ||
Hardly any RNA detectable (concentration < 10 ug/ml). Abysmal 260/280-ratio </br> | Hardly any RNA detectable (concentration < 10 ug/ml). Abysmal 260/280-ratio </br> | ||
| − | -> Heat-only lysis is too inefficient for that low cell counts, use 10 | + | -> Heat-only lysis is too inefficient for that low cell counts, use 10<sup>9</sup> next time. |
</li> | </li> | ||
</ul> | </ul> | ||
| Line 4,367: | Line 4,367: | ||
<ul> | <ul> | ||
<li> | <li> | ||
| − | Lysis at 90°C, used 300 uL of E. coli suspension diluted to 2*10 | + | Lysis at 90°C, used 300 uL of E. coli suspension diluted to 2*10<sup>9</sup> cells/ml |
</li> | </li> | ||
<li> | <li> | ||
| Line 4,535: | Line 4,535: | ||
<ul> | <ul> | ||
<li> | <li> | ||
| − | Diluted E. coli (DH5a-pSB1C3-Tev-008-His) suspension to 10 | + | Diluted E. coli (DH5a-pSB1C3-Tev-008-His) suspension to 10<sup>6</sup> cells / ml and heat-lysed in 50 uL aliquots |
</li> | </li> | ||
<li> | <li> | ||
| Line 5,099: | Line 5,099: | ||
<ul> | <ul> | ||
<li> | <li> | ||
| − | Diluted E. coli (DH5a-pSB1C3-Tev-008-His) suspension in a range of 10 | + | Diluted E. coli (DH5a-pSB1C3-Tev-008-His) suspension in a range of 10<sup>6</sup> to 10<sup>2</sup> cells / ml and heat-lysed in 50 uL aliquots |
</li> | </li> | ||
<li> | <li> | ||
| Line 5,108: | Line 5,108: | ||
<li class="myResults"> | <li class="myResults"> | ||
<h4>Results</h4> | <h4>Results</h4> | ||
| − | Bands visible down to a dillution of 5*10 | + | Bands visible down to a dillution of 5*10<sup>4</sup> or 10<sup>4</sup> cells/ml, which corresponds to an average of 100 or 20 cells' DNA per PCR |
<img style="margin-bottom: 15px" src="https://static.igem.org/mediawiki/2017/e/e0/T--Munich--pic--lysis_pcr_deltacells.png"> | <img style="margin-bottom: 15px" src="https://static.igem.org/mediawiki/2017/e/e0/T--Munich--pic--lysis_pcr_deltacells.png"> | ||
</li> | </li> | ||
Revision as of 11:31, 30 October 2017
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