Difference between revisions of "Team:Tel-Hai/Parts"

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<h3>Here are our three favorite parts containing the different versions of the KP6 toxin:</h3>
 
<h3>Here are our three favorite parts containing the different versions of the KP6 toxin:</h3>
 
<p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2408020" target="_blank">BBa_K2408020</a> - ADH1_KP6_proToxin: The toxin KP6 from U.maydis can bind to and damage the outer membrane of the B.bruxellensis yeast and thus cause cell death. KP6 is translated as a protoxin and is then <strong>cleaved</strong> by protease into 2 subunits, alpha and beta. We have designed the part with the MF(ALPHA)1 secretion signal,  ADH1 promoter, terminator and a histidine tag.</p>
 
<p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2408020" target="_blank">BBa_K2408020</a> - ADH1_KP6_proToxin: The toxin KP6 from U.maydis can bind to and damage the outer membrane of the B.bruxellensis yeast and thus cause cell death. KP6 is translated as a protoxin and is then <strong>cleaved</strong> by protease into 2 subunits, alpha and beta. We have designed the part with the MF(ALPHA)1 secretion signal,  ADH1 promoter, terminator and a histidine tag.</p>
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<p class="text-center">
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<img src="https://static.igem.org/mediawiki/2017/9/9d/T--Tel-Hai--01.jpg" alt="01">
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</p>
 
<p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2408021" target="_blank">BBa_K2408021</a> - ADH1_KP6_alphaSubunit: The KP6 toxin’s alpha subunit, which is <strong>cleaved</strong> by protease from the KP6 protoxin (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2408020" target="_blank">BBa_K2408020</a>). This sequence also contains the MF(ALPHA)1 secretion signal,  ADH1 promoter, terminator and a 6 His tag.</p>
 
<p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2408021" target="_blank">BBa_K2408021</a> - ADH1_KP6_alphaSubunit: The KP6 toxin’s alpha subunit, which is <strong>cleaved</strong> by protease from the KP6 protoxin (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2408020" target="_blank">BBa_K2408020</a>). This sequence also contains the MF(ALPHA)1 secretion signal,  ADH1 promoter, terminator and a 6 His tag.</p>
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<p class="text-center">
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<img src="https://static.igem.org/mediawiki/2017/9/94/T--Tel-Hai--02.jpg" alt="02">
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</p>
 
<p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2408022" target="_blank">BBa_K2408022</a> - ADH1_KP6_betaSubunit: The KP6 toxin’s beta subunit, which is <strong>cleaved</strong> by protease from the KP6 protoxin (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2408020" target="_blank">BBa_K2408020</a>). This sequence also contains the MF(ALPHA)1 secretion signal, ADH1 promoter, terminator and a 6 His tag.</p>
 
<p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2408022" target="_blank">BBa_K2408022</a> - ADH1_KP6_betaSubunit: The KP6 toxin’s beta subunit, which is <strong>cleaved</strong> by protease from the KP6 protoxin (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2408020" target="_blank">BBa_K2408020</a>). This sequence also contains the MF(ALPHA)1 secretion signal, ADH1 promoter, terminator and a 6 His tag.</p>
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<p class="text-center">
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<img src="https://static.igem.org/mediawiki/2017/7/7e/T--Tel-Hai--03.jpg" alt="03">
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</p>
 
<p>Here are the parts we have created over our lab work. All our parts have been optimized to suit the yeast we worked with, Sacharomyces cerevisae.</p>
 
<p>Here are the parts we have created over our lab work. All our parts have been optimized to suit the yeast we worked with, Sacharomyces cerevisae.</p>
 
<table id="parts-table">
 
<table id="parts-table">

Revision as of 12:55, 30 October 2017

Parts

Here are our three favorite parts containing the different versions of the KP6 toxin:

BBa_K2408020 - ADH1_KP6_proToxin: The toxin KP6 from U.maydis can bind to and damage the outer membrane of the B.bruxellensis yeast and thus cause cell death. KP6 is translated as a protoxin and is then cleaved by protease into 2 subunits, alpha and beta. We have designed the part with the MF(ALPHA)1 secretion signal, ADH1 promoter, terminator and a histidine tag.

01

BBa_K2408021 - ADH1_KP6_alphaSubunit: The KP6 toxin’s alpha subunit, which is cleaved by protease from the KP6 protoxin (BBa_K2408020). This sequence also contains the MF(ALPHA)1 secretion signal, ADH1 promoter, terminator and a 6 His tag.

02

BBa_K2408022 - ADH1_KP6_betaSubunit: The KP6 toxin’s beta subunit, which is cleaved by protease from the KP6 protoxin (BBa_K2408020). This sequence also contains the MF(ALPHA)1 secretion signal, ADH1 promoter, terminator and a 6 His tag.

03

Here are the parts we have created over our lab work. All our parts have been optimized to suit the yeast we worked with, Sacharomyces cerevisae.

Name Type Description Designer Length
BBa_K2408001 Coding TAL Yaakov Bulka 1590
BBa_K2408002 Coding 4CL Yaakov Bulka 1731
BBa_K2408003 Coding STS_his_S.cerevisiae_Optimized Yaakov Bulka 1197
MF_KP6_his Coding MF_KP6_his Yaakov Bulka 1173
BBa_K2408005 Coding MF_Miraculin_his Yaakov Bulka 1176
BBa_K2408010 Regulatory TDH3pro Yaakov Bulka 681
BBa_K2408011 Regulatory PGK1pro Yaakov Bulka 781
BBa_K2408012 Regulatory HXT7pro Yaakov Bulka 651
BBa_K2408020 Coding ADH1_KP6_proToxin Almog Angel, Michal Glikman 1870
BBa_K2408021 Coding ADH1_KP6_alphaSubunit Almog Angel, Michal Glikman 1189
BBa_K2408022 Coding ADH1_KP6_betaSubunit Almog Angel, Michal Glikman 1195
BBa_K2408023 Coding ADH1_Miraculin Almog Angel, Michal Glikman 1609
BBa_K2408024 Coding HXT7_miraculin Michal Glikman 1233
Tel-Hai