Difference between revisions of "Template:TU Darmstadt/Flowchart"

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             id="tspan1151"><a href="https://2017.igem.org/Team:TU_Darmstadt/project#two">Visit the subpage!</a></tspan></text>
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             id="tspan1151"><a
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              href="https://2017.igem.org/Team:TU_Darmstadt/project#two"
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              id="a88">Visit the subpage!</a></tspan></text>
 
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             id="tspan4665">tetrameric and pentameric chitin.</tspan><tspan
 
             id="tspan4665">tetrameric and pentameric chitin.</tspan><tspan
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              id="tspan1145"><a
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            id="tspan1145">Visit the subpage!</tspan></text>
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            href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase">Visit the subpage!</a></tspan></text>
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             style="font-size:4.23333311px;line-height:0.5em;stroke-width:0.26458332">we use NodB and COD.</tspan><tspan
 
             style="font-size:4.23333311px;line-height:0.5em;stroke-width:0.26458332">we use NodB and COD.</tspan><tspan
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            id="tspan1147">Visit the subpage!</tspan></text>
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            href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_deacetylase">Visit the subpage!</a></tspan></text>
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             style="font-size:4.23333311px;line-height:0.5em;stroke-width:0.26458332"
 
             style="font-size:4.23333311px;line-height:0.5em;stroke-width:0.26458332"
 
             id="tspan6374">bacteria within minutes.</tspan><tspan
 
             id="tspan6374">bacteria within minutes.</tspan><tspan
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              id="tspan1149"><a
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            id="tspan1149">Visit the subpage!</tspan></text>
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            href="https://2017.igem.org/Team:TU_Darmstadt/project/chemistry">Visit the subpage!</a></tspan></text>
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Revision as of 13:21, 30 October 2017

image/svg+xml 1. Chitosan:Chitosan is a derivate of the linear polysaccharide chitin. The chemical and physical properties can vary remarkably. This enables a huge scope of applications. Chitosan shows antimicrobial properties and supports scar free wound healing.Visit the subpage! 2. Wound infection:Simple and cheap wound treatment is a challenging task.The detection of potentially present pathogenic bacteria iscomplicated, as removal of the wound dressing disrupts thehealing process. 3. Chitin Synthase:To realize the synthesis of chitosan, for usage in wounddressing hydrogels, we produce chitin out of N-acetyl-glucosamine-UDP monomers. Chitin is a precursor for chitosan.We implement the chitin synthase NodC, which createstetrameric and pentameric chitin.Visit the subpage! 4. Chitin Deacetylase:To manufacture chitosan out of chitin, the producedchitin oligomers have to be deacetylated. This can beperformed by using certain hydrolyzing enzymes called chitin deacetylases. In our case,we use NodB and COD.Visit the subpage! 5. Chemistry:To deal with the problem of wound infections andtheir detection, our synthesized chitosan is linkedto a flourophor via a peptide linker. This makes itpossible to detect exoproteases from pathogenicbacteria within minutes.Visit the subpage!