Difference between revisions of "Team:SIAT-SCIE/Demonstrate"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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<h4> What should we do for our demonstration?</h4>
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<h5> Standard teams </h5>
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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<h5> Special track teams </h5>
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
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            <h2 style="font-size:x-large;">Overview</h2>
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            <br/>
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            <p style="font-family:'Avenir';font-size:23px">Our project is mainly focusing on:</p>
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            <br />
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            <ul class=" block">
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                <li style="margin-left:100px;font-family:'Avenir';font-size:23px;margin-bottom:10px">The ability of CAHS proteins in increasing the survival rate of desiccated bacteria</li>
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                <li style="margin-left:100px;font-family:'Avenir';font-size:23px;margin-bottom:10px">The ability of Dsup proteins in increasing the survival rate of irradiated bacteria</li>
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                <li style="margin-left:100px;font-family:'Avenir';font-size:23px">The amount of Dsup proteins in affecting the survival rate of irradiated bacteria</li>
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            <br />
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            <br />
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            <h2 style="font-size:x-large;">Experiment & Results</h2>
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            <br />
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            <h4 style="font-family:'Avenir';font-size:23px">Desiccation Tolerance Experiment</h4>
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            <br />
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            <p style="font-family:'Avenir';font-size:23px">After the vector construction and protein expression, we dilute the broth and spread it evenly to the growth culture, to count the Clone Forming Unit per 100μl of diluted broth. And use the same tube of broth, dehydrate it by centrifuge and desiccator. After leaving the dried broth for 48 hours in room temperature, we rehydrate it and spread it evenly to the growth culture again, and we can obtain the Clone Forming Unit per 100μl of diluted desiccated broth. By these two data, we can calculate the survival rate of the bacteria after desiccation.
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            </p><br /><h4 style="font-family:'Avenir';font-size:23px"><a href="https://2017.igem.org/Team:SIAT-SCIE/Results">Results:</a></h4><br />
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            <h5 style="font-family:'Avenir';font-size:23px">Optical Density of Broth</h5>
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            <br/><img src="https://static.igem.org/mediawiki/2017/3/36/SIAT-SCIE_proof1.png" alt="Optical Density" /><br /><br /><br />
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                    <h5 style="font-family:'Avenir';font-size:23px">Clone formed before desiccation</h5>
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                    <img src="https://static.igem.org/mediawiki/2017/e/e4/SIAT-SCIE_proof2.png" alt="Clone formed before desiccation" />
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                    <h5 style="font-family:'Avenir';font-size:23px"> Clone formed after desiccation</h5>
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                    <img src="https://static.igem.org/mediawiki/2017/e/e4/SIAT-SCIE_proof2.png"alt=" Clone formed after desiccation" /><br /><br /><br />
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                <img src="https://static.igem.org/mediawiki/2017/0/00/SIAT-SCIE_proof3.png" alt="whatwhatwhat">
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            <h4 style="font-family:'Avenir';font-size:23px">Radiation Tolerance Experiment</h4><br />
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            <p style="font-family:'Avenir';font-size:23px">The expression is induced by different concentration of IPTG, and use a microplate reader to obtain the fluorescence, which can indicate the amount of protein expressed. Then we dilute the broth, take 100μl of diluted broth, spread them on the growth culture, to obtain the original Clone Forming Unit per 100μl diluted broth. Then take 100μl of diluted broth, spread them on the growth culture. Place the growth culture under UV for different time periods, then grow over night, to obtain the Clone Forming Unit per 100μl of diluted irradiated broth. From these experiments, we can count the survival rates of the bacteria with different amount of Dsup protein expressed and survival rates of bacteria after different amount of radiation irradiated.
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            <br /><h4 style="font-family:'Avenir';font-size:23px"><a href="https://2017.igem.org/Team:SIAT-SCIE/Results" title="Results">Results: </a></h4><br />
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                    <h5 style="font-family:'Avenir';font-size:23px">OD:</h5>
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                    <img src="https://static.igem.org/mediawiki/2017/b/b6/SIAT-SCIE_proof4.jpg" alt="OD" />
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                    <h5 style="font-family:'Avenir';font-size:23px">Fluorescence</h5>
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                    <img src="https://static.igem.org/mediawiki/2017/b/b6/SIAT-SCIE_proof4.jpg" alt="Fluorescence" /><br />
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                    <h5 style="font-family:'Avenir';font-size:23px">Clone formed before irradiation</h5>
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                    <img src="https://static.igem.org/mediawiki/2017/0/00/SIAT-SCIE_proof3.png" alt="Clone formed before irradiation" />
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                    <h5 style="font-family:'Avenir';font-size:23px">Clone formed after irradiation</h5>
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                    <img src="https://static.igem.org/mediawiki/2017/0/00/SIAT-SCIE_proof3.png" alt=" Clone formed after irradiation" /><br />
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            <h2 style="font-size:x-large;">Conclusion:</h2><br/>
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            <p style="font-family:'Avenir';font-size:23px">Now, we are still doing our experiment, so it is hard to say whether our theory works. Let us pray that the paper is true and we did not make any mistake.
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{{SIAT-SCIE footer}}

Revision as of 13:43, 30 October 2017

comic

Overview


Our project is mainly focusing on:


  • The ability of CAHS proteins in increasing the survival rate of desiccated bacteria
  • The ability of Dsup proteins in increasing the survival rate of irradiated bacteria
  • The amount of Dsup proteins in affecting the survival rate of irradiated bacteria


Experiment & Results


Desiccation Tolerance Experiment


After the vector construction and protein expression, we dilute the broth and spread it evenly to the growth culture, to count the Clone Forming Unit per 100μl of diluted broth. And use the same tube of broth, dehydrate it by centrifuge and desiccator. After leaving the dried broth for 48 hours in room temperature, we rehydrate it and spread it evenly to the growth culture again, and we can obtain the Clone Forming Unit per 100μl of diluted desiccated broth. By these two data, we can calculate the survival rate of the bacteria after desiccation.


Results:


Optical Density of Broth

Optical Density


Clone formed before desiccation
Clone formed before desiccation
Clone formed after desiccation
Clone formed after desiccation


whatwhatwhat



Radiation Tolerance Experiment


The expression is induced by different concentration of IPTG, and use a microplate reader to obtain the fluorescence, which can indicate the amount of protein expressed. Then we dilute the broth, take 100μl of diluted broth, spread them on the growth culture, to obtain the original Clone Forming Unit per 100μl diluted broth. Then take 100μl of diluted broth, spread them on the growth culture. Place the growth culture under UV for different time periods, then grow over night, to obtain the Clone Forming Unit per 100μl of diluted irradiated broth. From these experiments, we can count the survival rates of the bacteria with different amount of Dsup protein expressed and survival rates of bacteria after different amount of radiation irradiated.


Results:


OD:
OD
Fluorescence
Fluorescence


Clone formed before irradiation
Clone formed before irradiation
Clone formed after irradiation
Clone formed after irradiation


Conclusion:


Now, we are still doing our experiment, so it is hard to say whether our theory works. Let us pray that the paper is true and we did not make any mistake.

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