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Revision as of 13:45, 30 October 2017
Introduction
Several protocols for specific procedures have been followed while working in the wet lab. These include protocols for PCR, purifications, digestions, assemblies as well as working with Saccharomyces cerevisiae and Escherichia coli. See the exact procedures in the lab journal.
Protocols
Protocols for PCR
Thermo Scientific DreamTaq DNA Polymerase
Thermo Scientific Phusion High-Fidelity DNA Polymerase
PrimeSTAR HS DNA Polymerase
Fusion PCR
Protocols for purification
Protocols for digestion and assembly
Protocols for Saccharomyces cerevisiae
Transformation (Chemical)
Transformation (Electroporation)
Transformation (Quick version)
Isolation of genomic DNA
Protocols for Escherichia coli
Protocols for Biobricks
Protocols for Interlab study
